Objective To establish an HPLC method for the simultaneousdetermination of 12 common bacteriostatic agents (benzoic acid, benzylalcohol, sorbic acid, methylparaben, thimerosal, ethylparaben,chlorhexidine acetate, propyl hydroxybenzoate, butyl p-hydroxybenzoate,benzalkonium bromide, domiphen bromide, benzalkonium chloride ) inbrimonidine tartrate eye drops. The 48 batches of samples collected from4 domestic manufacturers were tested and evaluated. Methods ShiseidoCAPCELL PAK C18 MGII column ( ${4.6}\mathrm{\;{mm}}\times {250}\mathrm{\;{mm}},{5\mu}\mathrm{m}$ ) was used, ${0.02}\mathrm{\;{mol}}/\mathrm{L}$ ammonium acetate(pH adjusted to 4.5 by glacial acetic acid)(mobile phaseA) and methyl alcohol(mobile phase B) were used for gradient elution,and the detection wavelength was ${262}\mathrm{\;{nm}}$ . Results Theseparation degree of 12 bacteriostatic agents reached the requirements,and the blank excipients did not interfere with the determination ofbacteriostatic agents, the linear relationship of bacteriostatic agentsin their linear range was good, the average recoveries were in the rangeof 92.8% to 105.7% and the RSDs were ${0.3}\%\sim {1.9}\%\left({\mathrm{n}=9}\right)$ . The qualitative analysis showed that the types ofbacterial inhibitors in 48 batches were all in agreement with theformulation. The quantitative determination showed that the contents ofbacterial inhibitors were between ${24}\%\sim {102}\%$ of the formulation. Conclusion The method is sensitive, rapid and specific, and can be usedfor the accurate quantitative determination of multiple bacteriostaticagents in brimonidine tartrate eye drops. This study can providetechnical support for the standardized use of bacteriostatic agents inthis variety.

The re evaluation of qualification recognition capability is a capability confirmation work that inspection and testing institutions need to carry out every six years. The new version of the "Criteria for Qualification Recognition and Review of Inspection and Testing Institutions" has been officially implemented since December 1, 2023. This article mainly elaborates on how to efficiently pass the qualification recognition re evaluation work of inspection and testing institutions under the requirements of the new criteria. Taking a certain inspection and testing institution in Beijing as an example, this article briefly introduces the entire process of re evaluation, including organization preparation, online reporting, on-site audit, later rectification, and evidence collection. It provides a detailed analysis of the entire process of re evaluation and points out the precautions to be taken when preparing application materials for re evaluation. This provides a reference for the qualification recognition re evaluation application and related work of inspection and testing institutions.

Objective To verify the experimental method of erythromycin ointment. Methods Three batch numbers of erythromycin ointment (S1~S3) were selected and divided into three washing methods: test group and positive and negative control group (test article control group and bacterial solution control group). The microbial limit detection method of erythromycin ointment was analyzed by test. Results The recovery ratio of oxygen bacteria, yeast, mold and added bacteria required for different batches of erythromycin ointment in the test is between 0.5 and 2, and the inhibition of microbial strains of erythromycin ointment test can be adopted by membrane filtration method. Therefore, all the methods can effectively eliminate the antibacterial components of erythromycin ointment, and the experimental results can truly reflect the microbial contamination of erythromycin dye ointment. Conclusion The application of centrifugal method in antibacterial removal will not affect the microbial limit test of erythromycin ointment.

Objective To establish a high performance liquidchromatography method for the determination of the content uniformity ofpromethazine hydrochloride, and to provide a reliable experimental basisfor the subsequent study of the content uniformity and content qualityof pediatric drugs after manual breaking tablets. Methods Highperformance liquid chromatography was used, octadecyl silane bondedsilica gel was used as the column filling agent, water (adjusted pH to2.3 with glacial acetic acid)-methanol (55 : 45) was used as the mobilephase, and the detection wavelength was ${254}\mathrm{\;{nm}}$ . ResultsPromethazine hydrochloride showed a good linear relationship in therange of 17.392~26.088 μg/mL, and the linear equation was: $\mathrm{Y}= {55272}\mathrm{X}+{348938}\left({{r}^{2}= {0.9986}}\right)$ . The method recoveryrate was between 100.22% and 101.40%, and the RSD was ${0.49}\%$ . The content uniformity ofpromethazine hydrochloride was in accordance with the provisions of thefour general principles of the Chinese Pharmacopoeia 2020 edition.Finally, this method was used to analyze the accuracy of children’sdrugs by different methods of dosing by the content and contentuniformity of promethazine hydrochloride tablets. Conclusion The method has strong specificity in determining the content uniformity and content of promethazine hydrochloride, and the accuracy and precision are good. The dose accuracy of children’s tablets is reduced by many times of dosing, and it also provides very important data theoretical and practical significance for promoting the dose accuracy of children’s tablets.

University laboratories are characterized by their complex functions, high safety risks, and significant management challenges. Establishing a sound safety education and training model is an important means to promote the safety management of laboratories. This study discusses the current issues in safety education and training for university laboratories, including the structural issue that safety education and training are not incorporated into the laboratory management system, the process issue of having a singular form of safety education and training and a lack of targeted content, and the result issue of the absence of an evaluation mechanism for safety education and training. In response to these issues, a university laboratory safety education and training model based on structure-process-result is constructed around pre-experiment, during-experiment, and post-experiment stages, providing references for improving the safety literacy of laboratory personnel and ensuring the safe operation of laboratories.

As an important energy resource, the quality of coal directly affects combustion efficiency, environmental emissions, and product quality. Traditional detection methods rely on manual sampling and laboratory testing, which have issues such as uneven sampling, cumbersome operations, and long cycles, making it difficult to meet real-time and accurate requirements. This paper proposes optimization solutions including multimodal information fusion, standardization and automation, and intelligent monitoring systems. These technologies significantly improve the accuracy and efficiency of coal quality detection and have been validated in both laboratory and industrial applications, demonstrating their great potential in real-time monitoring and management of coal quality. The optimized methods not only more accurately reflect the actual ash and sulfur content in coal products but also provide strong technical support for the development of the coal industry, achieving intelligent and efficient coal quality monitoring.

Water resources are an important foundation for Chinese national economy and social development, and water quality and environmental monitoring are of great significance for ensuring water resource security and water environment protection. Microbial detection technology, as a fast, accurate, and sensitive water quality monitoring method, has been widely applied in the field of water quality monitoring in China. In order to improve the effectiveness of microbial detection and enhance the monitoring of water quality and environment in China, this article will adopt literature review and practical research methods to analyze the commonly used microbial detection technologies and compare their advantages and disadvantages, aiming to provide scientific basis for water quality and environmental protection in China.

With the rapid development of medical education, medical laboratory plays a crucial role in cultivating medical talents and scientific research innovation. However, due to the complexity and specificity of laboratory management, traditional management methods are no longer able to meet the needs of modern laboratory management. This article aims to explore the application of standardized management measures in the management of medical school laboratories. Through in-depth analysis of the current situation of medical school laboratory management, a series of standardized management measures are proposed to improve the efficiency of laboratory management, ensure the safe and stable operation of laboratories, and provide strong guarantees for medical education and scientific research innovation.

Objective To investigate and analyze the microbial contamination in the production and processing of pre-packaged sauced meat products in Sichuan Province. Methods The total number of bacterial colonies, coliform, Staphylococcus aureus, Listeria monocytogenes, Salmonella and Bacillus cereus were detected in 52 samples from a pre-packaged spiced meat factory in Sichuan province by national standard method. Results The results showed that the meat products were contaminated by Escherichia coli, Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes during the curing process. The detection rate of microorganisms gradually decreased with the process of processing, and high temperature sterilization had a significant effect on reducing the microbial pollution of the final product; By tracing analysis, it was found that the environment and equipment in the raw and cooked area were contaminated by Listeria monocytogenes of the same serotype. Conclusion The factory has a big problem of staff hygiene, raw food area and cooked food area cross-contamination, it is recommended that enterprises strictly control the production environment and personnel hygiene, regular cleaning and disinfection, reduce the pollution of processing environment and equipment, workshop operators and food cross-contamination and raw and cooked food cross-contamination, in order to reduce the risk of product microbial contamination.

Objective to analyze the impact of the application ofLaboratory Information Management System (LIMS) in laboratory testquality management on the existence of test return pass rate. Methods328 specimens were selected from the specimens tested in our laboratoryfrom January 2022 to December 2023, and were divided into routine team(January 2022-December 2022, routine quality management of tests) andsurvey team (January 2023-December 2023, quality management of testscompleted by LIMS system) according to the time, with 164 specimens ineach team. Comparison of specimen test reporting, adverse events andlaboratory staff satisfaction among the two teams. Results Comparing thetest reporting of specimens in the two teams, the critical value returnrate (95.73%), the timely rate of critical value reporting (96.95%), andthe qualified rate of test return (97.56%) were higher in the surveyteam $\left({{\chi }^{2}={6.821},{4.525},{5.878}, P <{0.05}}\right)$ ; and in thecomparison of the incidence rate of adverse events among the teams, thesurvey team (3.05%) was lower $\left({{\chi}^{2}= {4.525}}\right.$ , $P<{0.05}$ ); compared with before the implementation of LIMSsystem, the scores of laboratory staff efficiency $\lbrack \left({{9.12}\pm {0.55}}\right)$ points], management effectiveness [(9.16±0.57) points], and risk control[(9.27±0.54) points] were higher after the implementation $\left({t ={2.369},{2.270},{2.291}, P<{0.05}}\right)$ . Conclusion Laboratory test qualitymanagement under LIMS can improve test return pass rate, critical valuereturn rate and critical value return timely rate, reduce the risk ofadverse events, and improve the satisfaction of laboratory staffmanagement, with significant practical value.