Objective This study aims to conduct a compatibility testbetween human fibrinogen and inner packaging materials throughaccelerated testing, explore the stability of drugs, determine whetherinner packaging materials have an impact on product quality, and providenecessary information for formulation design, packaging, transportation,and storage. Methods Conduct accelerated testing using 3 batches of testsamples, and place them upright and inverted at a temperature of $\left({{25}\pm 2}\right){}^{\circ }\mathrm{C}$ for6months. During the experimental period, samples were taken at theend of the third and sixth months respectively, and tested according tothe accelerated stability key inspection items (appearance, vacuumdegree, reconstitution time, visible foreign matter, osmotic pressuremolar concentration, stability test, moisture, $\mathrm{{pH}}$ value, purity, total amountof human fibrinogen, coagulation activity, arginine hydrochloride,citrate ions, sterility test), and compared with the results of Month 0. Results After being stored at ${\left({25}\pm 2\right)}^{\circ }\mathrm{C}$ for6months, the relevant migration products met the requirements, andthere were no significant changes in the activity indicators. Otherbiochemical detection indicators met the requirements of the ChinesePharmacopoeia (2020 edition, Part III), and there were no significantdifferences in the changes of the detection results of each indicator. Conclusion The selected packaging system has no adverse effects on thequality of human fibrinogen, and the drug quality in the selectedpackaging container is stable and controllable, which can maintain itssafety and effectiveness in use. The inner packaging material has nosignificant impact on the quality of the formulation and has goodcompatibility.

Laboratories in colleges and universities are important places for scientific practice, facing a large number of teachers and students, and involving the safety and stability of the campus environment. Aiming at the current situation of gaseous pollutant emission and management in teaching laboratories of colleges and universities at home and abroad, a comprehensive research has been carried out by means of web browsing, literature reading, field examination and questionnaire survey, etc. The research includes: types of gaseous pollutants in laboratories and methods of their disposal, the current situation and problems of gaseous pollutant emission in laboratories of domestic colleges and universities, the advantages of gaseous pollutant management in laboratories of foreign colleges and universities and the results of the analysis based on research Based on the results of the research, the study and the corresponding countermeasures are given, in order to provide reference for the emission and control of gaseous pollutants in teaching laboratories of universities in China.

Objective Based on UHPLC-Orbitrap HRMS, combined withQuECHERS pretreatment technology, a rapid screening detection method wasestablished to simultaneously screen and confirm 14 categories of 140illegally added drugs in herbal tea. Methods The sample was extractedwith methanol, and purified by dispersed solid phase extraction withsodium acetate and anhydrous magnesium sulfate, neutral alumina andoctadecyl bonded silica gel, separated by a chromatographic columnWaters BEH ${\mathrm{C}}_{18}\left({{2.1}\mathrm{\;{mm}}\times {50}\mathrm{\;{mm}},{1.7\mu}\mathrm{m}}\right)$ , and gradient eluted by two independentmobile phase systems: ${0.2}\%$ formicacid water $\left({V/V}\right)$ solution $\sim {0.2}\%$ formic acidacetonitrile $\left({V/V}\right)$ solution and water-acetonitrile, Full $\mathrm{{MS}}/{\mathrm{{dd}- {MS}}}^{2}$ mode was used to collect data, the standard curve was prepared byextracting solution from blank sample and the external standard methodwas used for quantification. Results The linear relationship of 20representative quality control compounds was good $\left({{r}^{2}> {0.995}}\right)$ from5.0 to ${200}\mathrm{{ng}}/\mathrm{{mL}}$ massconcentration, the average recovery rateat the three levels of low,medium and high is between 83.1% and 107.3%, the relative standarddeviation is less than 15.6% and detection low limit is between ${0.2}\mathrm{{mg}}/\mathrm{{kg}}$ and ${1.0}\mathrm{{mg}}/\mathrm{{kg}}$ .165 herbal tea samples on the market were determined and results showedthat twelve samples were positive, the positive components werechlorpheniramine, acetaminophen, diclofenac sodium, ibuprofen,dexamethasone acetate, erythromycin and florfenicol. Conclusion Themethod has the advantages of simple operation, high sensitivity andhigh-throughput screening. Is suitable for the detection of illegallyadded drugs in herbal tea and other health foods.

Objective To improve and establish a molecular fluorescencespectrophotometry method for determining the content of vitamin ${\mathrm{B}}_{2}$ in animal derived foods.Methods Using pork belly, chicken, pig liver, and cow kidney as researchobjects, and referring to GB 5009.85-2016 National Standard of FoodSafety-Determination of Vitamin ${B}_{2}$ in Foods, further simplified theexperimental process, optimize experimental conditions, analyzed variousfactors that affect the results during the detection process, such as $\mathrm{{pH}}$ , light intensity, andthe effect of sodium hydrosulfite on fluorescence quenching, improvedthe accuracy of the method for determining vitamin ${\mathrm{B}}_{2}$ in animal derived foods.Results The maximum fluorescence intensity was determined at pH 6.5. Asthe light intensity increased and the light duration increased, thefluorescence intensity gradually decreased. After 5 hours, vitamin ${\mathrm{B}}_{2}$ basically decomposedcompletely. Vitamin ${\mathrm{B}}_{2}$ exhibited good linearitywithin the range of $0 \sim {10\mu}\mathrm{g}$ , with ${r}^{2}={0.9993}$ ; the limit of detection was ${0.004}\mathrm{{mg}}/{100}\mathrm{\;g}$ ,and the limit of quantification was ${0.02}\mathrm{{mg}}/{100}\mathrm{\;g}$ .The recovery rates for spiking ranged from ${90}\%\sim {100}\%$ . The relativestandard deviation of parallel sample determination results was lessthan 6%. Conclusion This method is accurate, simple in equipment, andcost-effective, and can meet the needs of grassroots laboratories forthe determination of vitamin ${\mathrm{B}}_{2}$ in animal derivedfoods.

Objective To understand the pathogenic spectrum compositionof bacterial diarrhea in Jiangxia District of Wuhan, to explore thevariation and epidemic changes of the main pathogenic bacteria inJiangxia District of Wuhan in recent years, and to provide scientificbasis for the diagnosis and prevention and control of bacterialdiarrhea. Methods 341 specimens of patients with bacterial diarrheasyndrome in Jiangxia District of Wuhan from 2021 to 2023 were collected,and a variety of intestinal pathogenic bacteria were detected, and thedrug resistance of the detected pathogenic bacteria was analyzed. Results The highest detection rate in 2021-2023 was Salmonella,accounting for 42.82%(146/341) of cases detected; next is Vibrio, with adetection rate of ${16.42}\%\left({{56}/{341}}\right)$ ; thethird place is Escherichia coli, with a detection rate of 6.74%(23/341).In the typing results, the most commonly detected strains of Salmonella,Vibrio, and Escherichia coli were Salmonella enterica, Vibrioparahaemolyticus, and pathogenic Escherichia coli, with detection ratesof 14.37%, 13.20%, and 3.52%, respectively. Among the 341 detectedbacteria, the age group of 20~40 had the highest number of detectedbacteria, with 223, accounting for 65.40% of the total; 118 strains weredetected in the age group of 40~60, accounting for 34.60% of the total.There is a statistically significant difference between the two agegroups $\left({{\chi }^{2}= {57.484}, P<{0.001}}\right)$ . Conclusion Salmonella is the main pathogenic bacteria of bacterial diarrhea in Jiangxia District of Wuhan from 2021 to2023.

Production safety is related to people's well-being and the overall situation of economic and social development. However, laboratory hazardous chemical safety-related incidents have occurred in recent years, which has sounded the alarm for laboratory safety management in colleges and universities. This paper focuses on the outstanding problems and weak links in the safety management of university laboratories and hazardous chemicals, and focuses on the work carried out by the university in the implementation of responsibilities, system construction, publicity and education, hazard source control, hidden danger investigation and rectification, emergency drill and information platform construction, so as to provide reference for the fine management of hazardous chemicals in university laboratories.

Objective To compare the application effects of different influenza virus detection methods. Methods 100 influenza patients admitted to a certain hospital from January 2021 to June 2023 were randomly divided into an observation group (fluorescence RT-PCR test) and a control group (colloidal gold immunochromatographic test), with 50 patients in each group. Results Compared with the control group, the observation group had a higher positive rate of testing (P&lt;0.05). Conclusion Using fluorescence RT-PCR to detect influenza virus can achieve higher detection results and improve the positivity rate of the test.

Objective By analyzing the original detection results of urine organic acids in Changchun, Jilin Province, this study aims to explore the incidence of genetic metabolic diseases related to urine organic acid detection projects in Changchun, Jilin Province, and analyze the detection data. Methods Gas chromatography-mass spectrometry (GC-MS) was used to analyze the results of the urine organic acid detection project submitted by Jilin Jincheng Medical Co., Ltd. in Changchun, Jilin Province from January 2019 to May 2023. Results there were 83 cases of positive diseases in the initial screening, with a positive rate of 3.93%. Among them, there were 43 cases of methylmalonic acidemia with a positive rate of 51.81%, 10 cases of hyperphenylalaninemia with a positive rate of 12.05%, 6 cases of glutaric acidemia type 1 with a positive rate of 7.23%, 5 cases of Citrin protein deficiency with a positive rate of 6.02%, 5 cases of propionaemia with a positive rate of 6.02%, 4 cases of maple diabetes with a positive rate of 4.82%, and 10 cases of other diseases with a positive rate of 12.05%. Conclusion Organic acid metabolism disease, also known as organic aciduria, is the most common genetic metabolic disease. Urine organic acid screening is an effective method for preventing and treating certain genetic metabolic diseases after birth, among which methylmalonic acidemia is the most common genetic metabolic disease with abnormal organic acid metabolism. In order to improve the survival rate and quality of life of children, people must raise awareness, detect, diagnose and treat them early.

Objective To evaluate the early diagnosis and prognosis ofmultiple myeloma (MM) by gene sequencing technology. Methods 68 patientswith MM admitted from May 2020 to May 2024 were randomly divided intoobservation group (34 cases) and control group (34 cases). Patients inthe observation group received early diagnosis and prognosis assessmentrelated to gene sequencing, while those in the control group receivedtraditional diagnosis methods. Results In the early diagnosis accuracy,the number of correct and wrong diagnoses in the observation group was31 cases and 3 cases, respectively, and the diagnosis accuracy was91.2%. The number of correct and wrong diagnoses in control group was 23cases and 11 cases, respectively, the diagnostic accuracy was ${67.62}\%$ , and the observation group washigher $\left({P <{0.05}}\right)$ .In the observation group, the number of ISS stage I, ISS Stage II, ISSStage III and high-risk mutation detection were 10 cases, 15 cases, 9cases and 12 cases, respectively, and the accuracy of prognosisassessment was 85.3%. In the control group, there were 8 cases of ISSstage I, 13 cases of ISS stage II, 13 cases of ISS stage III and 8 casesof high-risk mutation detection, respectively. The accuracy of prognosisassessment was ${67.6}\%$ , and theaccuracy of prognosis assessment in the observation group wassignificantly higher than that in the control group. The overallsurvival rate and progression-free survival rate were 94.1% and 70.6% inthe observation group, and 82.4% and 52.9% in the control group,respectively, which were higher in the observation group $\left({P <{0.05}}\right)$ . Conclusion Gene sequencing technology is helpful to the early detection of MM andthe formulation of personalized treatment plan, improve the survivalrate and quality of life of patients, and has the value of applicationand promotion in clinical practice.

Listeria monocytogenes (LM) is a foodborne pathogen that causes diseases with high rates of lethality, and its contamination is particularly problematic in ready-to-eat meat products. With the accelerated pace of modern life and the rapid development of society, the demand for ready-to-eat food has been rising, and the risk of contamination of ready-to-eat food by LM has increased. This not only poses a potential threat to the health and safety of consumers, but also has the potential to trigger significant economic losses. This paper comprehensively analyzed the outbreak cases of LM in ready-to-eat meat products, the relevant laws and regulations of ready-to-eat foods in specific countries, and the current biological control measures. Through the in-depth discussion of relevant elements, the aim was to comprehensively describe the control strategy of LM in ready-to-eat foods, and identified potential risks and challenges, so as to provide scientific guidance for the control of LM in the food industry.