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Objective Based on UHPLC-Orbitrap HRMS, combined withQuECHERS pretreatment technology, a rapid screening detection method wasestablished to simultaneously screen and confirm 14 categories of 140illegally added drugs in herbal tea. Methods The sample was extractedwith methanol, and purified by dispersed solid phase extraction withsodium acetate and anhydrous magnesium sulfate, neutral alumina andoctadecyl bonded silica gel, separated by a chromatographic columnWaters BEH ${\mathrm{C}}_{18}\left({{2.1}\mathrm{\;{mm}}\times {50}\mathrm{\;{mm}},{1.7\mu}\mathrm{m}}\right)$ , and gradient eluted by two independentmobile phase systems: ${0.2}\%$ formicacid water $\left({V/V}\right)$ solution $\sim {0.2}\%$ formic acidacetonitrile $\left({V/V}\right)$ solution and water-acetonitrile, Full $\mathrm{{MS}}/{\mathrm{{dd}- {MS}}}^{2}$ mode was used to collect data, the standard curve was prepared byextracting solution from blank sample and the external standard methodwas used for quantification. Results The linear relationship of 20representative quality control compounds was good $\left({{r}^{2}> {0.995}}\right)$ from5.0 to ${200}\mathrm{{ng}}/\mathrm{{mL}}$ massconcentration, the average recovery rateat the three levels of low,medium and high is between 83.1% and 107.3%, the relative standarddeviation is less than 15.6% and detection low limit is between ${0.2}\mathrm{{mg}}/\mathrm{{kg}}$ and ${1.0}\mathrm{{mg}}/\mathrm{{kg}}$ .165 herbal tea samples on the market were determined and results showedthat twelve samples were positive, the positive components werechlorpheniramine, acetaminophen, diclofenac sodium, ibuprofen,dexamethasone acetate, erythromycin and florfenicol. Conclusion Themethod has the advantages of simple operation, high sensitivity andhigh-throughput screening. Is suitable for the detection of illegallyadded drugs in herbal tea and other health foods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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