Colleges and universities incorporate the evaluation system into the laboratory safety management, which is very important for carrying out the laboratory safety management works effectively. It is not only meets the objective requirements of the state, but also is contribute to guide teachers and students to establish correct safety awareness and create great laboratory safety culture atmosphere. Based on the college safety requirements of the Ministry of Education, we could create an open, fair and equitable evaluation contents and selection process on the laboratory safety, and set up awards and rewards foe excellent laboratories. In this way to arouse the enthusiasm of teachers and students for the laboratory safety works, and gradually form laboratory safety culture.

Objective to analyze the impact of the application ofLaboratory Information Management System (LIMS) in laboratory testquality management on the existence of test return pass rate. Methods328 specimens were selected from the specimens tested in our laboratoryfrom January 2022 to December 2023, and were divided into routine team(January 2022-December 2022, routine quality management of tests) andsurvey team (January 2023-December 2023, quality management of testscompleted by LIMS system) according to the time, with 164 specimens ineach team. Comparison of specimen test reporting, adverse events andlaboratory staff satisfaction among the two teams. Results Comparing thetest reporting of specimens in the two teams, the critical value returnrate (95.73%), the timely rate of critical value reporting (96.95%), andthe qualified rate of test return (97.56%) were higher in the surveyteam $\left({{\chi }^{2}={6.821},{4.525},{5.878}, P <{0.05}}\right)$ ; and in thecomparison of the incidence rate of adverse events among the teams, thesurvey team (3.05%) was lower $\left({{\chi}^{2}= {4.525}}\right.$ , $P<{0.05}$ ); compared with before the implementation of LIMSsystem, the scores of laboratory staff efficiency $\lbrack \left({{9.12}\pm {0.55}}\right)$ points], management effectiveness [(9.16±0.57) points], and risk control[(9.27±0.54) points] were higher after the implementation $\left({t ={2.369},{2.270},{2.291}, P<{0.05}}\right)$ . Conclusion Laboratory test qualitymanagement under LIMS can improve test return pass rate, critical valuereturn rate and critical value return timely rate, reduce the risk ofadverse events, and improve the satisfaction of laboratory staffmanagement, with significant practical value.

This study aims to respond to the call of the Dali Bai Autonomous Prefecture Ecological Environment Bureau to intensify monitoring of the Erhai Lake area and its main rivers, and conduct long-term and continuous monitoring of the chemical oxygen demand (COD) and dissolved oxygen (DO) in the river channel entering Erhai Lake in Dali Manjiang. By deeply analyzing nearly 170 sets of monitoring data and drawing a relationship diagram between COD and DO, we have a deep understanding of the relationship between these two water quality indicators in the river channel entering Erhai Lake in Dali Manjiang. This study not only provides strong data support for further monitoring and control of the river channel entering Erhai Lake, but also provides a scientific basis for promoting the protection of Erhai Lake. By revealing the relationship between COD and DO, it is possible to more accurately assess the water quality of the river channel entering Erhai Lake, so as to more effectively formulate and implement environmental protection measures and contribute to the sustainable and healthy development of the ecological environment of Erhai Lake.

As an inevitable product in coal mine production, dust has a wide range of sources. These dust not only have complex physical and chemical properties, but their concentration and distribution characteristics are also affected by various factors. This article aims to comprehensively and systematically study the characteristics and hazards of coal mine underground dust through fixed-point sampling and detection analysis methods. By selecting and arranging sampling points, preparing and calibrating sampling instruments, and performing specific operations during the sampling process, the accuracy of data is ensured. At the same time, based on the detection results, the concentration, particle size distribution, chemical composition, and explosiveness of dust are revealed, in order to provide effective basis for the development of targeted dust prevention measures.

Objective To evaluate and analyze the value of halophilicbacteria culture in the detection of Vibrio parahaemolyticus in seafoodin Dalian area. Methods 46 samples of Marine products were collectedfrom Dalian from July 2023 to October 2023, and halophilic bacteria werecultured and isolated. The salt-tolerance test was carried out toidentify and analyze the virulence of Vibrio parahaemolyticus, and thecharacteristics and detection of Vibrio parahaemolyticus in Marineproducts were analyzed. Results A total of 45 halophilic and salttolerant strains were screened, with a yield of 44.44% at 20% salinity,55.56% at 15% salinity, and 11.11% at 50°C cultivation environment. Theisolated strains continued to grow at a concentration of ${25}\%\mathrm{{NaCl}}$ , and the bestgrowth was observed at a concentration of ${10}\%\mathrm{{NaCl}}$ . Seventeenmoderately halophilic bacteria were detected, with a total of 28 salttolerant strains. Conclusion The detection rate of Vibrioparahaemolyticus in Marine products in Dalian area can be improved byusing halophilic bacteria culture detection. The results of virulenceidentification show that Vibrio parahaemolyticus has strong virulenceand potential risk of food poisoning.

Objective To analyze the quality control results of detecting influenza virus nucleic acid by fluorescence real-time quantitative PCR. Methods From May 2021 to November 2022, 120 samples of influenza virus infection were studied. The RNA of influenza virus in these samples was extracted by nucleic acid and detected by PCR real-time fluorescence quantitative technique to evaluate its effect. Results 44 cases of influenza B virus, 40 cases of influenza A 1 virus and 36 cases of influenza A 3 virus were detected by this technique. All the test results meet the quality control standards of molecular biology operation, and the qualified rate reaches 100.0%. Conclusion Real-time nucleic acid PCR technology is widely used in clinical influenza virus detection. PCR real-time fluorescence quantitative detection method is accurate and rapid in the detection of influenza virus nucleic acid, which is suitable as the first choice to deal with public health emergencies.

Objective Exploring the influence of crystal form of ceftriaxone sodium on the quality of ceftriaxone sodium for injection products. Methods Analyze the relationship and reasons between crystal form and product quality by comparing the changes in polymer content of different crystal form products after long-term and accelerated testing. Results There are differences in the quality stability of different crystal forms of ceftriaxone sodium products. Conclusion Production enterprises should pay attention to the production process and choose the optimal crystal form to improve product quality

Objective To evaluate the analytical performance of serumamyloid A protein and C-reactive protein detection reagents based onmicrofluidic technology, so as to determine whether they can meetclinical requirements. Methods The precision, accuracy, linear range,interference test and other technical indicators of the detectionreagent were determined according to the CLSI EP scheme. Results Theintra-batch coefficients of variation of high and low concentrationsamples were 0.54% and 1.79%, and the inter-batch coefficients ofvariation were 0% and 1.20%, respectively, for the serum amyloid Adetection reagent; the intra-batch coefficients of variation of high andlow concentration samples were 0.30% and 2.28%, and the inter-batchcoefficients of variation were 0.17% and 0%, respectively, for theC-reactive protein detection reagent; the experimental results of themethodological comparison of the two methods were $\mathrm{Y}=$ -0.0609+0.9871X, ${\mathrm{R}}^{2}= {0.9975}$ and $\mathrm{Y}= {0.3354}+{0.9857}\mathrm{X},{\mathrm{R}}^{2}= {0.9990}$ , respectively,and the allowable errors were greater than the upper limit of theexpected bias confidence interval, and the biases were acceptable; thelinear range of the serum amyloid A detection reagent was $3 \sim {100.0}\mathrm{{mg}}/\mathrm{L}$ ,and the linear range of the C-reactive protein detection reagent was $3\sim {150.0}\mathrm{{mg}}/\mathrm{L}$ . Conclusion The analytical performance of serum amyloid A and C-reactive protein detection reagents based on microfluidic technology can meet the needs of clinical laboratories.

The purpose is to study the vertical linear uncertainty of ultrasonic flaw detectors. Firstly, introduce the specific instrument models and technical parameters, analyze the basis and methods for evaluating the vertical linear uncertainty of ultrasonic flaw detectors, and conduct uncertainty evaluation through modeling. Finally, issue a vertical linear uncertainty report and propose the key points for calibrating the ultrasonic flaw detector calibration device. Intended to provide reference for ultrasonic detection personnel, master the exploration skills of vertical linear uncertainty of ultrasonic flaw detectors, truly optimize the quality and performance of ultrasonic flaw detectors, and truly play its functional role.

With the improvement of people's living standards, food safety issues have become the focus of attention. Food microorganisms as an important indicator of food hygiene, which has an important role in ensuring food safety. To a large extent, determine the accuracy of the test results. This article conducts research and discussion of process control from the entire link of the medium procurement, acceptance, storage, preparation, use, and abandonment of the medium, which explains the measures to improve the quality of the medium. It is expected to provide some references for the effective development of food microorganisms.