Objective To compare the role of enzyme-linkedimmunosorbent assay and toluidine red unheated serum test in thediagnosis of syphilis. Methods 78 suspected syphilis patients in ourhospital were analyzed from January 1st, 2023 to December 31st, 2023,enzyme linked immunosorbent assay and toluidine red unheated serum testwere performed on all patients, and the testing procedures were strictlyfollowed according to the instructions. Compare the diagnosticsensitivity, specificity, and accuracy of enzyme-linked immunosorbentassay (ELISA) and toluidine red unheated serum test based on the resultsof syphilis Treponema pallidum gelatin particle agglutination test.Results The sensitivity, specificity, and accuracy of the enzyme-linkedimmunosorbent assay were 93.24%, 75%, and 92.31%, respectively. Thesensitivity, specificity, and accuracy of the toluene red unheated serumtest were 82.43%, 50%, and 80.77%, respectively. The toluene redunheated serum test was lower than the enzyme-linked immunosorbentassay, with a difference of $P<{0.05}$ . Conclusion Enzyme linked immunosorbent assay(ELISA) has higher sensitivity and accuracy in syphilis testing comparedto the non heated serum test with toluidine red, and is worthy ofattention and promotion.

Objective To add data output and processing functions to ordinary models of UV visible spectrophotometers. Methods Connect the spectrophotometer to an Android system phone using a serial port to USB cable. The phone uses serial software to receive and process the measurement data sent by the spectrophotometer. Results The connection between the spectrophotometer and the mobile phone was successfully achieved. The mobile phone can smoothly receive and store the output data of the spectrophotometer, and perform graphical processing, synchronously drawing the wavelength absorbance scanning spectrum of the sample. The obtained absorption spectrum is basically consistent with the commercial instrument. Conclusion A simple modification scheme for the output and processing of ordinary spectrophotometer data based on Android system smartphones has been proposed. The modification method is low-cost, easy to operate, and ordinary UV visible spectrophotometers with serial interfaces can be upgraded and modified.

University laboratory as an important base of scientific research and teaching, safety management is very important. Effective laboratory management plays an important role in ensuring the smooth operation of practical teaching and scientific research and the safety of teachers and students. This study focuses on the current situation and challenges of the safety management of specialized biological laboratories related to agriculture, focusing on four core elements of laboratory management system, reagent management, equipment management and waste liquid treatment. Through the construction of scientific management system, explore the safety management system of biological laboratories related to agriculture, aiming to improve the safety management ability of scientific research laboratories in local universities under the background of new agricultural science.

As a new supervision mode, intelligent supervision is being explored in the field of food safety in China. By integrating modern information technologies such as big data, cloud computing, the Internet of Things and artificial intelligence, intelligent supervision aims to realize the whole-process monitoring, risk early warning and efficient response to food safety. This study aims to explore the application and prospect of smart supervision in the field of food safety. By integrating modern information technology, smart supervision can effectively improve supervision efficiency, reduce food safety risks, and enhance public confidence in food safety. At the same time, for the challenges faced by smart regulation, this paper discusses how to improve the legal and regulatory framework of smart regulation, strengthen technological innovation, enhance public participation, and how to build a multi-participation regulatory mechanism to ensure the healthy development of smart regulation in the field of food safety in China.

Objective Establish a ultra high performance liquidchromatography (UPLC) method for the simultaneous determination of sevenginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd in ginseng medicinalmaterials. Methods Using CAPCELL PAK ${\mathrm{C}}_{18}\mathrm{{MG}}\left({{2.0}\mathrm{\;{mm}}\times {150}\mathrm{\;{mm}},{2.0\mu}\mathrm{m}}\right)$ Chromatographic column with gradientelution of acetonitrile-0.05% phosphoric acid solution as mobile phase,flow rate of ${0.38}\mathrm{\;{mL}}\cdot {\mathrm{{min}}}^{-1}$ ,detection wavelength of ${203}\mathrm{\;{nm}}$ , column temperatureof ${30}^{\circ }\mathrm{C}$ , andinjection volume of ${1\mu}\mathrm{L}$ . Results All 7 ginsenosides can be completelyseparated and have a good linear relationship $\left({{r}^{2}> {0.999}}\right)$ withina certain linear concentration range, with an average recovery rate of ${97.22}\%\sim {104.56}\%$ ; Therelative standard deviation (RSD) is ${0.4}\%\sim {1.8}\%\left({n = 9}\right)$ ,and the precision RSD is ${0.57}\%\sim {1.42}\%$ . Conclusion Thismethod has strong specificity, excellent precision, good accuracy, andmore efficient detection process, and can be used for the determinationof seven ginsenosides in ginseng medicinal materials.

With the continuous deepening of the national institutional reform process, the social functions of drug regulatory departments have become increasingly prominent, and there is an urgent need to strengthen the implementation of responsibilities, improve the work framework system, and enhance the effectiveness of drug adverse reaction monitoring. Based on this, this article first introduces the importance of strengthening the monitoring of adverse drug reactions and analyzes the composition of the elements of adverse drug reaction monitoring. On the basis of exploring the current situation and difficult issues of adverse drug reaction monitoring by drug regulatory authorities, combined with relevant practical experience, this paper proposes methods and countermeasures for drug adverse reaction monitoring by drug regulatory authorities under institutional reform, including improving the establishment of monitoring institutions and strengthening the management of reporting units.

With the development of China’s economy, people’srequirements for water quality are getting higher and higher. In orderto protect water resources, we need to do a good job of water qualitymonitoring, real-time monitoring of water quality indicators, timelyfind the trend of water quality changes, and analysis and prediction.The accurate determination of the permanganate index $\left({\mathrm{{COD}}}_{\mathrm{{Mn}}}\right)$ ,as a key indicator for evaluating the degree of pollution by organicmatter and reductive inorganic matter in water bodies, is of greatsignificance. The purpose of this paper is to discuss the qualitycontrol methods during the determination of permanganate index in waterin order to improve the accuracy and reliability of the results.

Informatization construction is a necessary means to realize the quality management of ecological environmental monitoring. This paper took the construction practice of the ecological environment monitoring quality management system in Guangxi as an example, combined with the problems existing in inspection and testing institutions, and used information technology to propose a system construction and design based on quality management as the goal. Quality control was carried out from multiple perspectives such as monitoring process, standard methods, data review, system judgment, and process supervision, realizing the full process management and traceability of ecological environment monitoring, accelerating the construction of standardized, standardized, refined, intelligent, and precise quality management, which helped to promote the high-quality development of ecological environment intelligent monitoring.

Quality control of cement concrete raw materials in highway engineering is a key factor in ensuring engineering quality and durability. This paper analyzes the importance, content and existing problems of cement concrete raw material testing and inspection, and proposes a series of quality control measures. These measures include formulating scientific and reasonable testing and inspection processes, strengthening the management of raw material use and inspection, and optimizing the inspection environment. By taking these measures, the quality of cement concrete engineering can be effectively improved, and the smooth construction and long-term use of highway engineering can be guaranteed.

Objective To establish an extraction process and contentdetermination method for quercetin in Gentiana macrophylla, and optimizethe processing technology of Gentiana macrophylla. Methods This studyused ultrasound assisted extraction to extract quercetin from Gentianamacrophylla, and determined the content of quercetin in four processedGentiana macrophylla decoction pieces by HPLC-ELSD. Results The optimalprocess conditions for ultrasonic extraction of quercetin from Gentianamacrophylla are: ethanol volume fraction of 75%, ultrasonic time of 45minutes, solid-liquid ratio of 1 : 10, and ultrasonic temperature of ${30}^{\circ }\mathrm{C}$ . Thehighest yield of quercetin is ${3.40}\mathrm{{mg}}/\mathrm{g}$ . Conclusion The established extraction process and content determinationmethod for quercetin galls have good repeatability, stability, andreliability, which can provide reference for optimizing the processingtechnology of Gentiana macrophylla decoction pieces.