Objective Goal by verifying the performance indicators of16 items such as alanine aminotransferase (ALT), the performance of thedetection system of BS-2800M, a fully automatic biochemical analyzerproduced by Mindray manufacturer, which was newly put into use in thislaboratory was evaluated, so as to improve the work quality of thelaboratory and ensure the accuracy and reliability of the test results. Methods According to the accuracy, precision, linear range and referenceinterval of the test items, the performance of 16 test items such asalanine aminotransferase (ALT) was evaluated. No unacceptableperformance was found in the evaluation process, indicating that theanalytical performance of the test method was accepted and met therequirements of clinical specimen detection. The entire validationprocess is based on the Requirements of the Guidelines for theAccreditation of Quality and Competence of Medical Laboratories in theField of Clinical Chemistry Examination, referring to the requirementsof CLSI EP15-A3, EP6, C28-A2 and EP15-A23 documents. Results Therelative bias of 16 items was $<1/2$ total allowable error (TEa). The coefficient of variation(CV) in precision batch was $<1/4$ tea, the total CV was $<1/3$ tea, the b values of 16 items were in the range of ${1.00}\pm {0.03}$ , and ${r}^{2}$ was $\geq {0.95}$ . The maximum dilution andclinical reportable range were verified and met the requirements. Conclusion The accuracy, precision, linearity and clinical reportablerange of the 16 tested items meet the requirements of quality objectivesand can be used for clinical detection.

Production safety is related to people's well-being and the overall situation of economic and social development. However, laboratory hazardous chemical safety-related incidents have occurred in recent years, which has sounded the alarm for laboratory safety management in colleges and universities. This paper focuses on the outstanding problems and weak links in the safety management of university laboratories and hazardous chemicals, and focuses on the work carried out by the university in the implementation of responsibilities, system construction, publicity and education, hazard source control, hidden danger investigation and rectification, emergency drill and information platform construction, so as to provide reference for the fine management of hazardous chemicals in university laboratories.

Objective To analyze the effectiveness of enzyme-linkedimmunosorbent assay (ELISA) and colloidal gold test in the detection ofhuman immunodeficiency virus (HIV) antibodies. Methods From January 2022to January 2024,50 cases of sentinel surveillance and contact populationof confirmed AIDS patients in our unit were selected as the observationtarget, and they were detected by ELISA and colloidal gold testrespectively, and the difference of patients’ detection results wasanalyzed. Results Among the 50 key populations, 36 were ultimatelydiagnosed and 14 were negative, which is the gold standard for thisstudy. The diagnostic effect of enzyme-linked immunosorbent assay issignificantly better than that of colloidal gold assay, and thedifference is statistically significant $\left({P <{0.05}}\right)$ . Conclusion The enzyme-linked immunosorbent assay (ELISA) is significantly moreeffective in HIV antibody detection than colloidal gold assay, and isworthy of being the preferred detection method.

Taking Jiangnan University as the practice subject, the paper studies the interdependence of the preparation, procurement, use and disposal stages in the full life cycle management system of large scientifiic research instruments and equipment.Taking this as the cornerstone, in view of the main problems existing in the equipment management. The improving measures are proposed including promoting information construction, making good budget demonstration, improving the performance appraisal, widening the channel of disposal, strengthening the team construction. The present study aimed at by means of actively exploring the fine full life cycle management of large scientifiic research instruments and equipment, improving management level of instruments and equipment in colleges and universities in the new era to better service for teaching and scientific research.

The development of intelligent sensor technology has brought revolutionary changes to all walks of life, especially in the field of industrial production and textile testing to show great potential. Smart sensors are not only capable of sensing physical or chemical quantities in the environment, but also have data processing and intelligent analysis capabilities, making them a significant advantage in textile defect detection. This paper analyzes the basic principle, characteristics and application status of intelligent sensor in industrial production and textile testing. The applications and advantages of different types of intelligent sensors, such as vision, infrared and ultrasonic systems, in textile production are analyzed.

Objective To analyze the application results offluorescence quantitative reverse transcription-polymerase chainreaction (RT-PCR) in the detection of pathogenic microorganisms. MethodsA retrospective analysis was made of 7502 microbiological test dataexamined in the laboratory of our hospital from January 14th, 2023 toDecember 14th, 2023. Conventional RT-PCR method and fluorescentquantitative RT-PCR method were performed respectively, and the goldstandard was the results of special staining microscopy. The diagnosticefficiency and other indicators of the two test results were analyzed.Results ① The ratio of negative to positive by gold standard was ${490}: {7012}$ , the ratio of negative topositive by conventional RT-PCR was ${479}:{6358}$ , and the ratio of negative to positive by fluorescencequantitative RT-PCR was 488 : 7010. ② The sensitivity, specificity andaccuracy of fluorescence quantitative RT-PCR method were 99.97%, 99.59%and 99.95%, which were higher than that of conventional RT-PCR method,with statisticalsignificance $\left({P<{0.05}}\right)$ . ③ The detection rates of variousmicroorganisms by fluorescence quantitative RT-PCR were 99.91%, 100.00%,100.00%, 99.91%, 100.00%, 100.00%, 99.97%, higher than that byconventional RT-PCR, with statistical significance ( $P <{0.05}$ ). Conclusion Fluorescencequantitative RT-PCR method has high diagnostic efficiency and detectionrate in pathogenic microorganisms, which can provide scientific basisfor doctors’ diagnosis and treatment and can be implemented.

Objective To improve and establish a molecular fluorescencespectrophotometry method for determining the content of vitamin ${\mathrm{B}}_{2}$ in animal derived foods.Methods Using pork belly, chicken, pig liver, and cow kidney as researchobjects, and referring to GB 5009.85-2016 National Standard of FoodSafety-Determination of Vitamin ${B}_{2}$ in Foods, further simplified theexperimental process, optimize experimental conditions, analyzed variousfactors that affect the results during the detection process, such as $\mathrm{{pH}}$ , light intensity, andthe effect of sodium hydrosulfite on fluorescence quenching, improvedthe accuracy of the method for determining vitamin ${\mathrm{B}}_{2}$ in animal derived foods.Results The maximum fluorescence intensity was determined at pH 6.5. Asthe light intensity increased and the light duration increased, thefluorescence intensity gradually decreased. After 5 hours, vitamin ${\mathrm{B}}_{2}$ basically decomposedcompletely. Vitamin ${\mathrm{B}}_{2}$ exhibited good linearitywithin the range of $0 \sim {10\mu}\mathrm{g}$ , with ${r}^{2}={0.9993}$ ; the limit of detection was ${0.004}\mathrm{{mg}}/{100}\mathrm{\;g}$ ,and the limit of quantification was ${0.02}\mathrm{{mg}}/{100}\mathrm{\;g}$ .The recovery rates for spiking ranged from ${90}\%\sim {100}\%$ . The relativestandard deviation of parallel sample determination results was lessthan 6%. Conclusion This method is accurate, simple in equipment, andcost-effective, and can meet the needs of grassroots laboratories forthe determination of vitamin ${\mathrm{B}}_{2}$ in animal derivedfoods.

Objective In order to prevent the volatilization loss of volatile oil components such as bornyl acetate in Amomum villosum powder during storage, a reasonable and effective storage conditions and storage validity period of Amomum villosum powder were developed, so as to improve the quality stability and food efficiency of terminal products. Methods The samples of Amomum villosum powder in different storage environments [high temperature (35°C), cool (10~15°C) and cold storage (0~5°C)] were sampled every week, and the contents of bornyl acetate and volatile oil were determined respectively. Finally, the trend analysis was carried out according to the content results. Results According to the experimental results, the contents of bornyl acetate and volatile oil in Amomum villosum powder under different storage conditions were quite different. The contents of bornyl acetate and volatile oil in Amomum villosum powder under cold storage conditions were slightly higher than those under shade conditions, but they were significantly higher than those under high temperature storage conditions. Conclusion The research results provide an in-depth analysis of the changes in the main components of Amomum villosum powder under different storage conditions, providing important reference for enterprises to choose the storage method of Amomum villosum powder.

Objective This study aims to establish an efficient andaccurate method for the determination of zinc content in mineral samplesbased on the flame absorption method of atomic absorptionspectrophotometer, in order to meet the growing demand for metal elementdetection in various fields. Methods The zinc content was determined byatomic absorption spectrophotometer, combined with pretreatment samples,experimental procedure, optimization of instrument measurementconditions and establishment of standard curve. Results The establishedmethod has high precision and accuracy, the recovery rate is99.1%~99.9%, and the correlation coefficient is 0.9998 . The detectionlimit of the method was ${0.1}\mathrm{{\mug}}/\mathrm{{mL}}$ , indicating that the method had goodquantitative analysis ability. Conclusion The method has not only highsensitivity and selectivity, but also simple operation and reliableresults, which is of great significance for environmental protection andoptimal utilization of resources.

In order to build a comprehensive and in-depth university laboratory safety management system and systematically eliminate laboratory safety hazards, this article takes 259 core literature on university laboratory safety management systems collected from "China National Knowledge Infrastructure" from 2008 to 2024 as the basic data, and uses Cite Space software to visualize the research authors, research institutions, keywords, and other aspects, further analyzing the research status and development trends. The results indicate that the number of publications in this field has been increasing year by year, but there is relatively little interaction among researchers, and research institutions are relatively independent with unclear collaborative relationships. Research hotspots mainly focus on safety awareness, system construction, risk assessment, safety culture, emergency management, security system, and other aspects. In the future, emphasis should be placed on building laboratory safety management systems from these hotspots.