In recent years, under the intensifying global warming, extreme weather has occurred more frequently in Guangxi, presenting higher temperature and less rainfall in summer and autumn. The worsened drought has seriously affected sugarcane production. Scientifically selecting an appropriate planting time is an important way to cope with the summer and autumn drought. A sugarcane field test was conducted at the Shatang National Agricultural Meteorological Experimental Station in Liuzhou, Guangxi in 2021 and 2022. By utilizing typical hot and dry seasons in summer and autumn, the field experiments were conducted with three sugarcane varieties and the seedcanes were planted at seven different times from February to May to study the impact of summer and autumn hot and dry conditions on sugarcane yield traits at different planting times. The experimental results indicated that: (1) Under the conditions of high temperature and drought in summer and autumn, delayed planting tended to decrease the stalk length, stalk diameter, single stalk weight, and theoretical cane yield at harvest. Planting time A₁ in 2021 and 2022, the stalk length of each variety was 178–227 cm, stalk diameter was 27.6–30.3 mm, and single stalk weight was 1.05–1.42 kg, while planting time A₇, the stalk length was only 76–145 cm, stalk diameter was 21.5–30.0 mm, and single stalk weight was 0.26–0.85 kg. (2) Planting sugarcane in March resulted in the cane yield between 76.65 t/hm² and 104.10 t/hm². Sugarcane could utilize the favorable meteorological conditions at the early stages to alleviate the impact of summer and autumn heat and drought on sugarcane. The sugarcane crops planting from April to May was significantly affected by drought, Planting sugarcane on May 30th with a theoretical yield of only 18.90 t/hm² and 63.75 t/hm², resulting in a significant decrease in cane yield.(3) Sugarcane varieties ROC22 and GL05136 had advantages over GT42 in stalk length, single stalk weight, and cane yield, but GT42 had advantage in stalk thickness, meaning ROC22 and GL05136 having stronger drought resistance. Therefore, under the conditions of high temperature and drought in summer and autumn, suitable drought resistant sugarcane varieties should be selected, and sugarcane should be planted as early as possible to accelerate the early growth and development progress of sugarcane. Before the onset of summer and autumn drought, robust plants should be obtained to improve their drought resistance, in order to minimize the impact of meteorological disasters, and achieve high and stable yields.

The study investigated the effects of different water and fertilizer combinations on yield, quality, and economic benefits of pomelo under the condition of water and fertilizer integration to provide a basis for exploring and practicing the integrated fertilization model of water and fertilizer for pomelo through a 3-year fixed position experiment. Four treatment methods were set up for Pinghe honey pomelo yield orchard: conventional fertilization (TF), 30% water and fertilizer package (30%WF), 50% water and fertilizer package (50%WF), and 70% water and fertilizer package (70%WF). The results showed that the effect of different degrees of reduced fertilization with a water-soluble fertilizer package on the yield of pomelo was significant. The average yield was as follows: 70%W>50%WF>TF>30%WF. The average 3-year yield increase rate of 50%WF, and 70%WF treatments in high-yield orchards was 12.43%, and 24.67%, respectively, compared to TF treatment. The average annual yield increase rate of 50%WF, and 70%WF treatments in low yield orchards compared to TF treatments was 15.05%, and 26.80%, respectively. The average 3-year yield of honey pomelo in high-yield and low yield orchards was the highest in the 70%WF treatment (high-yield orchards 67 845.5 kg/hm², low yield orchards 53 495.5 kg/hm²), and the yield increase in low yield orchards was greater than that in high yield orchards. 70%WF had the best 3-year average secondary fruit rate in both high and low yield orchards (5.71% in high yield orchards and 4.67% in low yield orchards). The application of different water-soluble fertilizer combinations in high-yield and low yield orchards had no significant effect on the granulation rate of pomelo flesh, but had a more significant impact on single fruit weight and flesh weight. Moreover, there was a significant interannual variation in the cracking rate of honey pomelo treated with different water-soluble fertilizer combinations. The 70% water-soluble fertilizer package significantly improved the basic agronomic traits of pomelo, increased single fruit weight, and reduced fruit cracking rate. The increase and decrease in soluble solids, soluble sugars, and titratable acidity of the four treatments in high-yield and low yield orchards after 2 years were not significant, and there was no significant difference among the treatments. The 70% water-soluble fertilizer package had the best quality indicators for honey pomelo. The 3-year average final net income of high-yield orchards treated with 50%WF, and 70%WF increased by 30.58%, and 47.79% compared to the TF treatment, respectively. The 3-year average production to investment ratio was the highest in the 70%WF treatment (4.29∶1), which is 1.31 times higher than that of the TF treatment. The 3-year average final net income of low yield parks treated with 50%WF, and 70%WF increased by 40.41%, and 59.67% compared to the TF treatment, respectively. The 3-year average production to investment ratio was the highest in the 70%WF treatment (3.63∶1), which is 1.17 times higher than that of the TF treatment. The 70% water-soluble fertilizer package treatment had the highest economic benefits in both high and low yield orchards, with the economic benefits of low yield orchards increasing more than those of high yield orchards. Under the integrated mode of water and fertilizer, different water and fertilizer combinations with reduced fertilization had the effect of increasing the yield and economic benefits, improving the quality of honey pomelo, and the application of 70%WF treatment in high and low yield orchards had the best effect on improving the yield and economic benefits, and improving the quality of honey pomelo. This is the best fertilization treatment and is recommended for application.

The study was aimed to investigate the effects of fruit bagging on disease and pest incidence, pesticide usage rate and quality of fruits in the cultivation of jackfruit (Artocarpus heterophyllus) and to provide support for the high-quality and high-efficiency cultivation technique of jackfruit. With ten cultivars of jackfruit used as the experimental materials, the incidence of disease and pest and the number of new wormholes were calculated for bagging and non-bagging fruits. At the same time, the appearance quality such as the color of fruit pericarp and pulp were evaluated, and the content of soluble solid was determined. Moreover, the frequency and amount of pesticide application in the whole growing season were counted. The results showed that the rhizopus rot incidence of non-bagging fruits was 10.48%-25.00%, the rhizopus rot incidence in bagging fruits was only 0-2.62%, and the rhizopus rot incidence was reduced by 77.55%-100.00% in bagging fruits of ten cultivars. Similarly, the anthracnose incidence of all cultivars of non-bagging and bagging fruits was 10.00%-30.24% and 0-4.56% respectively, and the anthracnose incidence of bagging fruits decreased by 69.99%-100.00%. In addition, the incidence of jackfruit borer in all cultivars ranged from 23.33%-37.50% in non-bagging fruits and 4.00%-8.33% in bagging fruits, with the incidence of bagging fruits decreased by 74.62%-88.35%, in which the number of new wormholes and insects in bagging fruits of each cultivar was significantly less than those in non-bagging fruits. Compared to the non-bagging fruit, the bagging fruit was fuller in shape, the fruit surface was smoother, and the pericarp color was uniform. The non-bagging fruits had disadvantages of deformed fruit shape and uneven pericarp color. Bagging had no significant effect on the soluble solids content. By bagging the fruit, the frequency of pesticide application was reduced by 5 times and the amount of pesticide application was reduced by 85.87%. In summary, fruit bagging can be used as an eco-friendly management method of jackfruit, which can reduce the application of chemical pesticides while reducing the disease and pest of jackfruit, and maintaining the fruit quality.

Luffa, a major melon vegetables in China, is a crop of gourd family and the silk gourd genus, which is rich in nutrients and favored by consumers. With the increasing number and type of luffa germplasm resources, it is increasingly difficult to screen and preserve the excellent materials of luffa. In order to construct a core germplasm bank of luffa, eliminate germplasm materials with serious homogenization, and improve the efficiency of lucerne breeding, this study investigated the genetic diversity of 244 luffa germplasm resources by using 18 phenotypic traits that are highly representative of luffa as indicators, and compared and analyzed eight different sampling ratios (5%-40%), and three different sampling methods (random cluster sampling, group cluster sampling and preferential cluster sampling) were compared to analyze the representativeness of the core germplasm of luffa candidates. The results showed that the phenotypic genetic variation of lucerne germplasm resources was relatively rich, of which the largest Shannon index was leaf width, 3.0, and the smallest was seed melon length, 1.8. The most suitable sampling ratio was 20% using the comprehensive analysis of the seven evaluation indexes, and the most suitable sampling method was group cluster sampling. Using this method for core germplasm library construction, the primary core germplasm obtained containing 51 lucerne germplasm resources, accounting for 20.90% of all the original germplasm resources, and compared with the original germplasm, 13 of the 18 agronomic traits were in full agreement with the original germplasm in terms of the maximum and minimum values, and the characteristic values of most traits were the same as those of the original germplasm or the differences were relatively small. The T-test showed that the mean values of all phenotypic traits had no significant differences with the original germplasm, indicating that the core germplasm library constructed by the method has good representativeness and rich genetic diversity. This study could provide basic support for the collection, preservation and innovative utilization of lucerne germplasm resources in the later stage, and also lays a foundation for improving the efficiency of lucerne variety improvement.

Guangxi spans the southern subtropics and central subtropics, with the Tropic of Cancer crossing the central part of the province, and belongs to the subtropical monsoon climate zone, which breeds rich wild sugarcane germplasm resources. From 2016 to 2023, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences organized a team of researchers to carry out systematic surveys and collection of Narenga porphyrocoma germplasm resources in Guangxi. The team collected a total of 58 copies of N. porphyrocoma germplasm resources in 43 townships of 18 cities and districts in Guangxi. The results showed that the N. porphyrocoma germplasm resources in Guangxi were mainly distributed in the area of 60.0-602.5 m above sea level, longitude E: 107°08′01″-111°32′02″, latitude N: 23°08′02″-26°01′43″. The genetic diversity indices of the 20 descriptive quality traits of N. porphyrocoma germplasm resources in Guangxi ranged from 0 to 1.2044, with a mean value of 0.5557, in which the diversity of the color of the internodes after exposure was the highest, that of the cork was lower, and the diversity of the three traits of stem shape, aerial roots and water splitting was not reflected; the genetic diversity indices of N. porphyrocoma germplasm resources in Guangxi of the different collection areas ranged from 0.1040 to 0.4911, with Hezhou being the highest and Baise being the lowest. The phenotypic traits of N. porphyrocoma germplasm resources in Guangxi were rich in variation, and the coefficients of variation for six quantitative traits ranged from 17.3% to 53.6%, with an average of 34.8%; the coefficients of variation of N. porphyrocoma germplasm resources in Guangxi from different collection areas ranged from 29.4% to 36.9%, with Hechi being the largest and Guilin being the smallest. The results of cluster analysis showed that the 58 N. porphyrocoma germplasm resources in Guangxi were classified into six major groups, but were not closely related to the geographical origin, in which the materials in the first major group had better overall traits and excellent biomass and quality traits. The collection expanded the number of resources in Guangxi Sugarcane Germplasm Repository and would provide excellent genetic resources for sugarcane breeding and genetic improvement.

Auricularia villosula Malysheva is a similar species of Auricularia heimuer F. Wu, B.K. Cui & Y. C. Dai, mainly distributed in high altitude areas of tropical and subtropical regions. It can adapt to the high temperature and high humidity climate in Guangxi. There are few reports on the domestication of A. villosula. In this paper, the fruiting bodies of auriculae collected in the field was used, and the molecular marker was established by sequence analysis. The effects of different temperature, carbon and nitrogen sources on the mycelia growth of A. villosula were explored through single factor tests, and the carbon and nitrogen sources of the medium were further optimized by an orthogonal test. The fruiting body of fungus was preliminarily identified as A. villosula (TY081). The results showed that the developed molecular labeling method could quickly and effectively identify A. villosula. The optimal temperature for mycelia growth of A. villosula TY081 was 30 ℃, the optimal carbon source was glucose, and the optimal nitrogen source was soybean powder. The optimized medium carbon and nitrogen combination was fructose, yeast powder, mycelium growth rate reached 0.92 mm/d. A. villosula TY081 was incubated at 28 ℃ in a culture bag mainly composed of sawdust, and the bag was full for 60 days. After puncturing, it was cultured in a greenhouse for 10 days to form the primordia. The fruity body growth temperature was 24 ℃, the humidity was 85%, and the yield of each bag was 20.943 g (dry weight).

Crossbreeding research for Camellia species groups and intragroups was carried out to breed new varieties of Camellia with high ornamental value. A total of 14 species and varieties from Sect. Chrysantha Chang, Sect. Camellia (L.) Dyer, Sect. Archecamellia Sealy and Sect. oleifera Chang, Sect. Corallina Sealy were used to design 42 hybridization combinations, including 8 hybridization of species groups, 24 intragroup hybridization and 10 reciprocal hybridization combinations. 274 setting fruits from 2016 flowers pollinated in 42 combinations were observed, and the percentage of fruit setting rate was 13.6%. 72 hybrid fruits were harvested, and the setting rate was 3.57%. 104 hybrid seedlings were obtained from the 199 seeds harvested, and the seedling emergence percentage was 52.26%. The combination of C. polyodonta♀×C. semiserrata♂ had the highest number of hybrid seedlings, reaching 45, followed by C. semiserrata♀×C. polyodonta♂ with 14 hybrid seedlings, C. polyodonta♀×C. amplexicaulis♂ with 13 hybrid seedlings. The size, shape and luster of the leaves of most hybrid seedlings were in the middle of the parents, and the hybrid seedlings obtained from distant hybridization often showed some characteristics that could be used as early identification of the authenticity of hybrid seedlings. The compatibility of C. polyodonta♀×C. semiserrata♂, C. polyodonta♀×C. amplexicaulis♂, and C. polyodonta♀×Camellia chrysantha♂ hybrid combinations was good, with pollination numbers of 32, 30, and 53, respectively, and seed setting rates of 12.5%, 10.0%, and 22.6%, respectively. More pollination numbers led higher seed setting rate and stronger representativeness. C. polyodonta and C. semiserrata could be used as paternal or maternal to cross breeding, which showing a high reproduction in the inside group of hybridization and outside group of distant hybridization. C. polyodonta and C. semiserrata were good hybrid parents. whether C. amplexicaulis as paternal or maternal parent, there was good cross compatibility with Sect. C. chrysantha and C. osmantha were suitable as paternal parents.

Spinetoram is a common pesticide used to control thrips on mango. The addition of pesticide spray adjuvants can improve the utilization efficacy of the pesticide. Reasonable selection and use of additive products are crucial to improve the utilization efficiency of pesticides and reduce the use of pesticides. In this study, the wetting and spreading behavior of different types of pesticide adjuvants on the surface of mango leaves was investigated by measuring the static and dynamic surface tension, contact angle, spreading area, and evaporation time of spinetoram solution after adding five different types and different concentrations of pesticide adjuvant products. The results showed that adding different adjuvants could effectively reduce the static and dynamic surface tension of the spinetoram solution, reduce the contact angle and increase the spreading area of the solution on the leaf surface. Except polymer adjuvants (Noposion Tiandun) showing evaporation resistance, other adjuvant products shortened the evaporation time. The wettability of different types of adjuvants on mango leaves was not consistent, organosilicone (Jirun) had the best wettability, followed by metal silicate composite salt (Maidao), modified vegetable oil (Maifei), orange peel essential oil (Danluofeng), and polymer (Noposion Tiandun). By comparing the wetting and spreading behavior of different treatments on the front and back of mango leaves, it was found that the back of mango leaves was more difficult to be wetted by pesticide solution than the front. Therefore, the physicochemical properties of the solution and the surface structure of the leaves were important factors affecting the wetting behavior of the spray droplets on the leaves. The mango thrips often feed on the back of the leaves, so in the prevention and control of mango thrips, the appropriate concentration should be selected according to the type of adjuvants and the harm characteristics of thrips to achieve a better control effect. The results revealed the wetting and spreading behavior of different additives on mango leaves and provided data support for the rational use of pesticide adjuvants.

Xinluo district of Longyan city serves as the political, economic, and cultural center of the western region of Fujian. With the acceleration of urbanization and the close exchange of people and goods with surrounding cities, invasive plants have invaded the local area, posing a serious threat to biological safety. This study aims to assess the prevalence of invasive alien plants in urban green spaces and evaluate the region’s risk awareness and response capacity, thereby informing the development of effective management and control strategies. Field surveys, literature reviews, and the application of the quadrat method were utilized to collect information on alien invasive plants in Xinluo district and construct an invasion risk assessment system. The DPSIR model combined with the analytic hierarchy process (AHP) was employed to determine the weights of evaluation indicators, and a comprehensive assessment was conducted on the driving forces of invasion, adaptability to the local environment, distribution within the locality, impact on the local area, and response measures of the alien plants. Based on the grading standards and risk values, alien invasive plants were categorized into three invasion levels (from I to III). A total of 65 alien invasive plant species were identified in the urban green spaces of Xinluo district, belonging to 22 families and 52 genera. The Asteraceae family had the highest number of species, accounting for 29.23%, followed by Amaranthaceae (12.31%) and Euphorbiaceae (9.23%). There were five life forms, with annual herbaceous plants being the most prevalent, totaling 32 species and representing 49.23% of the total. Geographically, the Americas were the primary source of these plants, with 48 species accounting for 73.85% of the total. The analysis of invasion pathways revealed that unintentionally introduced plants were the most numerous, with 35 species, constituting 53.85% of the total species count. According to the invasion status assessment results, there were 16 high-risk invasive plants (level III), 18 medium-risk (level II), and 31 low-risk (level I). While there are a considerable number of alien invasive plant species in the urban green spaces of Xinluo district, the overall invasion risk level is relatively low, with most being in the medium to low risk categories (level II and III). It is recommended to focus on the control and prevention of the most harmful species, such as Bidens alba and Erigeron canadensis, based on the risk level.

Based on the fact that ethephon (an ethylene releaser) stimulation can significantly increase latex yield per tapping, it is traditionally believed that rubber biosynthesis is positively regulated by ethylene. Based on the critical role of jasmonic acid signaling in promoting the biosynthesis of secondary metabolites in plants, jasmonic acid signaling is also thought to play a role in the positive feedback regulation of natural rubber biosynthesis. Recent studies suggest that tapping-enhanced rubber biosynthesis is closely related to the activation of jasmonate signaling in laticifer cells of rubber tree. However, it remains unclear whether there is a crosstalk between the jasmonic acid signaling pathway and the ethylene signaling pathway in the regulation of rubber biosynthesis. For this purpose, in the present study, in vitro rubber biosynthesis efficiency of whole latex and small rubber particles (SRPs) from short rested trees treated by either methy jasmonates or ethylene were detected by using substrate ¹³C-MVA. qPCR was used to analyze the expression of four jasmonic acid signaling genes, HbCOI1, HbJAZ1, HbMYC1, HbMYC2, four ethylene-responsive element binding factors (ERFs) genes HbERFⅢa, HbERFⅦa, HbERFⅨc, HbERFⅩa, four rubber biosynthesis genes, HbHMGR1,HbSRPP1, HbREF1, HbHRT2 in laticifer cells after epicormic shoots were treated with either methy jasmonates or ethylene. The results showed that exogenous methyl jasmonate treatment significantly up-regulated the expression levels of jasmonic acid signaling genes HbCOI1, HbJAZ1, HbMYC1, HbMYC2, and rubber biosynthetic protein genes HbHMGR1,HbSRPP1, HbREF1, HbHRT2 in laticifer cells of rubber tree, and promoted rubber biosynthesis, but had little effect on the expression of ethylene-responsive element binding factor genes HbERFⅢa, HbERFⅦa, HbERFⅨc, HbERFⅩa, or even inhibited the expression. On the contrary, exogenous ethylene treatment significantly up-regulated the expression levels of ethylene-responsive element binding factor genes HbERFⅢa, HbERFⅦa, HbERFⅨc, HbERFⅩa in laticifer cells of rubber tree, but had little effect on the expression of jasmonic acid signaling genes HbCOI1, HbJAZ1, HbMYC1,HbMYC2 and rubber biosynthetic protein genes HbHMGR1, HbSRPP1, HbREF1, HbHRT2 or even inhibited the expression and rubber biosynthesis. The results suggest that there exists an antagonistic effects of jasmonic acid and ethylene on rubber biosynthesis in H. brasiliensis, which would provide a theoretical basis for elucidating the crosstalk of jasmonic acid signaling and ethylene signaling on regulation of natural rubber production.