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Recent advances in R2 retrotransposon−mediated large−fragment gene integration technology
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Yuze WANG1, 2, Bangwei MAO1, 2, Shengqiu LUO1, 2, Yangcan CHEN1, 3, Wei LI1, 2, 3, *
Science & Technology Review | 2026, 44(11) : 24 - 32
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Science & Technology Review | 2026, 44(11): 24-32
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Recent advances in R2 retrotransposon−mediated large−fragment gene integration technology
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Yuze WANG1, 2, Bangwei MAO1, 2, Shengqiu LUO1, 2, Yangcan CHEN1, 3, Wei LI1, 2, 3, *
Affiliations
  • 1State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100049, China
  • 2University of Chinese Academy of Sciences, Beijing 100049, China
  • 3Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China
Published: 2026-06-13 doi: 10.3981/j.issn.1000-7857.2025.06.00017
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Gene editing refers to the process of specifically modifying organism's genome using specific technical approaches to regulate its genetic information and phenotypic characteristics. Among them, large−fragment gene integration technology enables the accurate insertion or replacement of large exogenous DNA fragments in the organismal genome, which provides core technical support for the development of innovative therapeutic strategies against severe diseases. Starting from the historical origin of this technology, this paper systematically sorts out its developmental trajectory and analyzes the current technical bottlenecks of large−fragment gene integration technology, including exogenous DNA dependence, delivery challenges and prominent immune risks. Given that the novel large−fragment integration techniques based on R2 retrotransposons are expected to become a key solution to break through these limitations, the study further introduces the structural characteristics and retrotransposition mechanisms of R2 retrotransposons, and reviews the research advances in its structural analysis and engineering modification, and summarizes the technical breakthroughs and application prospects of R2 retrotransposon−mediated large−fragment integration tools, which provides theoretical insights and practical references for the application of R2 retrotransposon tools in gene therapy.

gene integration  /  genome engineering  /  R2 retrotransposon  /  RNA technology  /  gene therapy
Yuze WANG, Bangwei MAO, Shengqiu LUO, Yangcan CHEN, Wei LI. Recent advances in R2 retrotransposon−mediated large−fragment gene integration technology[J]. Science & Technology Review, 2026 , 44 (11) : 24 -32 . DOI: 10.3981/j.issn.1000-7857.2025.06.00017
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Article Info
doi: 10.3981/j.issn.1000-7857.2025.06.00017
  • Receive Date：2025-06-04
  • Online Date：2026-06-23
  • Published：2026-06-13
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  • Received：2025-06-04
  • Revised：2026-05-18
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Affiliations
    1State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100049, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
    3Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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