This article studies the impact of treatment frequency of the Tiaojing Cuyun acupuncture (acupuncture for regulating menstruation and promoting pregnancy) in the treatment of premature ovarian insufficiency (POI) based on real-world evidence.Using a prospective cohort study design, 100 POI patients were categorized into strong exposure and weak exposure groups based on different levels of acupuncture stimulation, with a primary outcome event of a 10 IU/L decrease in follicle stimulating hormone (FSH).Demographic and clinical characteristics of the cases were analyzed, and serum hormone levels before and after treatment, Kupperman scale scores, peak systolic velocity (PSV), and resistance index (RI） were compared with a logistic regression model to determine the optimal threshold of acupuncture stimulation exposure.The results indicated that the total acupuncture treatment duration of 6 weeks was the most sensitive dividing point, with 6 weeks or longer being considered as the high-exposure group.There were 73 cases in the high-exposure group and 27 cases in the low-exposure group.Before and after treatment, the FSH levels decreased in both groups, while the levels of estradiol (E2) and anti-Müllerian hormone (AMH) significantly increased (P<0.05).The Kupperman scores and RI decreased while PSV increased in both groups.Furthermore, the strong exposure group showed superior efficacy compared to the weak exposure group.Logistic regression analysis indicated that age, age at menarche, and the number of acupuncture treatments were correlated with efficacy (P<0.05).The "Tiaojing Cuyun" acupuncture technique can effectively improve premature ovarian insufficiency, and the efficacy is even better with a total acupuncture treatment duration of 6 weeks or longer.

Tiaojing Cuyun acupuncture  /  premature ovarian insufficiency  /  modified Kupperman scale score  /  serum sex hormone level  /  B ultrasound  /  real word study