The anterior cruciate ligament (ACL) is an important structure of the knee, easily getting hurt. Currently, it is a common practice using the autologous tendon graft to reconstruct the ACL and to treat the ACL injury. However, the tendonbone interface heals slowly after the ACL reconstruction, which affects the early rehabilitation of the patients. The Panax Notoginseng Saponins (PNS), a blood-activating and blood-stasis drug, is often used for the treatment of fractures. Our previous experiments show that the PNS can significantly enhance the osteogenesis effect of the tendon autograft in the reconstruction of the ACL. Thus, this paper focuses on the effect of the PNS on the osteogenic differentiation of the tendon derived stem cells (TDSCs). The TDSCs of passage 3 are divided into two groups, the PNS group and the negative control group. The effect of the PNS on the cell activity is detected by the flow cytometry and the CCK8. After 72 hours of the PNS treatment, the TDSCs are cultured with the osteogenic medium, and the expression of the osteogenic marker ALP is detected by the ALP staining and the Elisa. The TDSCs are subcutaneously injected into nude mice after the PNS treatment, and then the micro-CT is used to detect the ectopic osteogenesis 4 weeks later. Compared with the negative control group, it is shown that the PNS could promote the osteogenic differentiation of the TDSCs and the ectopic osteogenesis in nude mice.

panax notoginseng saponins  /  tendon derived stem cells  /  osteogenic differentiation