To investigate the effects of magnetic-mediated hyperthermia(MMH) in combination with Aidi injection on human lymphoma Daudi cells in vitro, the biocompatibility and temperature rising behavior of selected magnetic media are studied. Then cell proliferation in culture is studied in human lymphoma Daudi cell lines exposed to MMH and Aidi separately and in combination. The cell apoptosis and cycle of Daudi cells are analyzed by flow cytometry. Theoretical calculations indicate that the biocompatibility of MMH magnetic media is satisffactory. In vivo experiments it also generates heat effectively. The survival rate of Daudi cells exposed to MMH (300 kHz, 40 Gs, 30 min) is (78.48±0.95)%, but significantly decreases to (9.25±2.05)% (P<0.01) when being exposed to the combination of MMH and Aidi. MMH in combination with Aidi can promote Daudi cells apoptosis, but the change in cell cycle is not obvious. The MMH magnetic media is feasible to Daudi cells in vitro. MMH alone could induce apoptosis of Daudi cells and significantly enhance anti-tumor effect with Aidi injection.

magnetic-mediated hyperthermia  /  Aidi injection  /  human lymphoma Daudi cells  /  CCK-8  /  flow cytometer  /  apoptosis  /  cell cycle