To help identify marine fish and provide a reference standard for identification of Melanogrammus aeglefinus, a standard sample for Melanogrammus aeglefinus is established in this study. With collaboration of eight labs, concentration and quality of DNA are tested. The concentration value is (2.02±0.07) μg (k=2) and the purity value of DNA is 1.82±0.08 (k=2) which indicates good quality of samples. PCR and electrophoresis are used to test the uniformity, stability and value analysis of the samples. The results show that these samples in this study meet the technical requirements of national standards and that the method can be used for calibration and quality control of Melanogrammus aeglefinus.

Melanogrammus aeglefinus  /  molecular identification  /  standard sample  /  homogeneity  /  stability  /  uncertainty