Amphotericin B(AmB) is one of the most efficient antimycotic agents, thus widely used in clinical practice. In this study, Streptomyces nodosus ZJB15076 is used for AmB fermentation. The optimal carbon source, nitrogen source and fermentation conditions for the production of AmB are tested. The results show that glucose and nitrogen beef extraction is best for the fermentation of AmB. And the optimized fermenting conditions through single factor test are summed up as follows:culture temperature 28℃, initial pH value 7.0, inoculation 2% and liquid volume 40 mL/250 mL. The influence of adding glass bead is significant. After adding glass bead, the production of AmB is increased by 341.1% and reaches 4563.2 mg/L.

Amphotericin B  /  Streptomyces nodosus  /  carbon source  /  nitrogen source  /  optimization of fermentation  /  microparticles