Sodium-bile acid cotransporter (EgSBACT) functions in the sodium-bile acid transportation in Echinococcus granulosus (Eg) Protoscolex and the sequence is unknown. The specific primers for EgSBACT mRNA are designed and the EgSBACT gene is amplified by RT-PCR from Eg protoscolex total RNA. Then the EgSBACT gene fragment is cloned into pET30a vector which is a prokaryotic expression vector for sequencing and analyzing by bioinformatics method. The PCR products of EgSBACT is about 650 bp whose length is 654 bp and encodes a protein containing 217 amino acids and the pI of this protein is predicted as 9.28. Homology analysis shows that there is 43% of similarity between EgSBACT and the trematode SBACT (GenBank: GAA57409.1). Phylogenetic analysis shows that EgSBACT is clustered with Fluke, and has a lower homology to other species. EgSBACT gene is cloned from protoscolex of Echinococcus granulosus in Xinjiang, which lays a foundation for further study of EgSBACT function in the development and differentiation of PSCs into adult worms. This research provides a way to express the EgSBACT protein in vitro and taking advantage of this protein further research can reveal the exact functions of EgSBACT in the sodium-bile acid transportation in Echinococcus granulosus.

Echinococcus granulosus  /  EgSBACT  /  sequence analysis