This paper aims to optimize the method for content determination of monoester-type alkaloids in aconite by HPLC in the Chinese Pharmacopoeia (2010 edition). By comparing the methods mentioned in the Chinese Pharmacopoeia and in the literature, we found that there are defects in the extraction method of samples, preparation methods of sample solution, chromatographic conditions, detection methods and determination results in content determination of monoester alkaloids in aconite by HPLC. Therefore, we established a new determination method and compared it with the one stated in the Chinese Pharmacopeia. The established method had better performance than the previous one. This simple method greatly shortened the testing time, reduced the cost, and improved column efficiency, providing a safe, effective, and quality-controllable detection method for aconite.

aconite  /  the Chinese Pharmacopeia  /  HPLC  /  monoester alkaloids