This paper studies the anti-inflammatory, antitussive, and expectorant effects of traditional Uyghur medicinal herb Hyssopus officinalis L. extract by supercritical CO₂ fluid and its effect on pathological changes in lung tissue of asthmatic rats. The total number of cells and percentage of lymphocytes (Ly), eosinophils (Eos), and neutrophils (Neu) in BALF, the level of TGF-β1 in BALF and lung tissue were measured. The anti-inflammatory effect was studied by the external ear swelling of mice induced by dimethyl benzene. The antitussive effect was studied by the cough model induced by ammonia in mice. The expectorant effect was studied by a mouse model of phenoled test. They were sensitized with OVA, Al(OH)₃ and DPT vaccine and then were challenged with inhalation of aerosolized OVA solution for preparation of the asthma model. The level of lung tissue and BALF TGF-β1 were determined with ELISA, and the total number of cells and percentage of Ly, Eos, and Neu in BALF were counted by light microscopy. The experimental results showed that the extract significantly inhibited xylene-induced ear edema in mice (P<0.05), reduced the number of ammonia spray-induced cough in mice (P<0.05), and promoted the phenol red excretion of the trachea (P<0.05), so that the latency was significantly prolonged in asthmatic guinea pigs (P<0.05) and a dose-dependent trend was shown. As compared with those of the asthma model group, the level of TGF-β1, total number of cells and percentage of Ly, Eos, and Neu in BALF and the level of lung tissue TGF-β1 were significantly lower in the treated groups (P<0.05). The results indicated that Hyssopus officinalis L. extract had outstanding anti-inflammatory, antitussive, and expectorant effects, and it inhibited secretion of TGF-β1, Ly, Eos, and Neu, reducing the airway inflammation of asthma.

Hyssopus officinalis L. extract by supercritical CO₂ fluid  /  anti-inflammatory, antitussive and expectorant effects  /  airway inflammation of asthma