In this paper, an optimum mixture design is used for the preparation of cold-adapted multiple species inoculant. Based on the Design Expert software, a quadratic model is established as a function of the component fractions of species, such as the Saccharomyces cerevisiae, the Trichoderma viride and the flora A25-3, on the enzyme activity of the CMCase, the FPase, the cellobiohydrolase, the proteinase and the α-amylase. The response values satisfying all expectations are optimized,and the most excellent combinations of the Saccharomyces cerevisiae, the Trichoderma viride and the flora A25-3 are 0%, 37.24% and 67.76%, respectively. The result of the verification experiment on the formulation is consistent with the prediction. The straw degradation experiments show that the multiple species inoculant has a high level of degradation efficiency for the straw at a low temperature. It suggests that there is a great application prospect of the multiple species inoculant under cold conditions.

mixture design  /  multiple species inoculant  /  cellulase  /  cold-adapted  /  biomass waste