This paper studies the effect of Uyghur medicine abnormal savda munzip (ASM) on the proliferation, invasion and the expression of Rho/ROCK signal transduction pathway related proteins in human hepatocellular carcinoma (HepG2) cells. After being treated with ASM (10, 20, 25, 50 mg/mL) and Y-27632(10 μmol/L) for 24, 48 and 72 h, the proliferation of HepG2 cells was detected using the four methyl thiazolyl tetrazolium (MTT) method. The alteration of the cells invasive ability treated with ASM and Y-27632 for 24h was observed by scanning electron microscope (SEM) and cell invasion assay. Western Blot was used to determine the effect of these drugs on the expression of RhoA, ROCK1 and ROCK2. The results revealed that ASM suppressed the proliferation of HepG2 significantly with a dose-effect relationship: for groups of ASM with 10 and 20 mg/mL, inhibition increased with the time of ASM action; for groups of ASM with 25 and 50 mg/mL, no significant increase of inhibition was found. ASM suppressed the invasive ability of HepG2. SEM results showed that the formation and growth of hepatoma cellular pseudopodia were inhibited by ASM. ASM with doses 25 and 50 mg/mL decreased the expressions of ROCK1 and ROCK2 significantly, but there was no significant effect on RhoA expression. The study suggests that ASM suppresses the proliferation and invasive ability of human hepatoma HepG2, the mechanisms of which could be associated with less expression of ROCK.

abnormal savda munzip  /  Rho/ROCK  /  human hepatocellular carcinoma (HepG2)