The protective effect of Angelica keiskei chalcones on the damage of HaCaT cells induced by ultraviolet B(UVB) is investigated.. HaCaT cell with oxidative damage induced by ultraviolet B is incubated with Angelica keiskei chalcones at concentrations of 20 μmol/L, 10 μmol/L and 5 μmol/L for 24 h. The cell viability as well as the apoptosis rate are determined by MTT method and flow cytometer, respectively. The MDA content of cells and activities of SOD, CAT and Caspase-3 are determined by kit method. The mRNA expression of BCL-2 and Bax is evaluated by reverse transcription polymerase chain reaction (RT-PCR). Compared with the normal control, the cell viability, SOD, CAT activity and the expression level of BCL-2 mRNA in the irradiation model group are decreased while the MDA content, apoptosis rate, Caspase-3 activity and Bax mRNA are increased. The viability, SOD, CAT activity and BCL-2 mRNA expression of cells incubated with 20 μmol/L and 10 μmol/L are increased while the apoptosis rate, Caspase-3 activity and Bax mRNA expression are decreased, compared with the irradiation model group. All the differences are significant (P<0.05). Angelica keiskei chalcones may protect HaCaT cells from oxidative damage and decrease apoptosis rate by regulating the mRNA expression of BCL-2 and Bax.

Angelica keiskei chalcones  /  HaCaT cell  /  oxidative damage  /  apoptosis