This paper investigates the protective effects of dietary 5'-nucleotides on alcohol-induced kidney injury of mice. A total of 35 specific pathogen free (SPF) male Wistar mice are randomly divided into 5 groups given alcohol, equivalent distilled water or an isocaloric amount of dextrose intragastrically for 8 weeks. The two groups of alcohol-induced rats are fed with AIN-93G rodent diets including 0.04% and 0.16% 5'-nucleotides, respectively. Body weight (bw), renal function indexes and serum concentration of ICAM-1 and PCM-1 are measured, and renal pathologic changes stained by hematoxylin and eosin stain are observed by election microscope. The result shows that dietary 5'-nucleotides can reduce the rise of urinary protein per 24 h and level of blood serum creatinine (SCr) induced by alcohol, as well as the blood urea nitrogen (BUN) in the 0.16% NTs group. And compared to the alcohol group, the endogenous creatinine clearance are decreased significantly in the 0.04% and 0.16% NTs groups. The renal lesions present glomerular mesangium proliferation, inflammatory cell infiltration in tubule-interstitial and vacuolation or swell of renal tubule epithelium in the alcohol group, which are less in both NTs groups. The serum concentrations of ICAM-1 and PCM-1 of NTs groups are also lower than those of the alcohol group. What's more, the expression changes of PCX-1, TGF-β and fibronectin (FN) in kidney induced by alcohol are repaired by NTs supplement. In conclusion, the dietary 5'-nucleotides can protect kidney from injury induced by alcohol in mice.

nucleotides  /  alcoholic renal injury  /  protective effect  /  rats