A para-nitrophenol (PNP) degrading strain, named PN-1, was isolated from the PNP-contaminated soil. PN-1 utilized PNP as the sole carbon and nitrogen source during the cultivation. Based on the result of 16S rDNA gene sequence, the strain was preliminarily designated as Pseudomona. Experiments were carried out to investigate the degradation of PNP, heavy metal, antibiotic, plant growthpromoting (PGP) properties and GFP-tagging of PN-1. The results showed that the maximum tolerant dose of TM to PNP was 200 mg/L, and the MICs for the following four heavy metals were 700 mg/L for Cd²⁺, 500 mg/L for Pb²⁺, 800 mg/L for Zn²⁺, and 100 mg/L for Cr⁶⁺. What is more, PN-1 could grow in media with chloromycetin and ampicillin. PGP traits of PN-1 included IAA, siderophore production, ACC deaminase activity and phosphate solubilizing activity. GFP-tagged PN-1 could survive and bioremediate PNP-contaminated soil with plants.

paranitrophenol  /  heavy metal  /  antibiotic  /  plant growth promotion properties  /  GFP-tagging