In this paper, an experimental method of the central composite design is used to determine the kernel oil content in the Elaeagnus mollis preparation. Based on the Design Expert software, a quadratic model is established with independent variables of the kernel oil content, such as the extraction time, the extraction temperature and the material-liquid ratio. The response values satisfying all expectations are optimized, and the most excellent extraction conditions of the Elaeagnus mollis kernel oil are 45 min for the extraction time, 88℃, for the extraction temperature and 1:5 for the material-liquid ratio. Under these conditions, the actual extraction rate of the kernel oil is 92.44%. The result of the verification experiment for the formulation is consistent with the prediction. The fatty acids in the Elaeagnus mollis kernel oil are analyzed by GC-MS. The results show that 28 kinds of fatty acids and 9 kinds of unsaturated fatty acids are identified. They are mainly composed of 12.86% methyl oleic, 10% methyl palmitate, pentadecanoic, 9.78% acid methyl ester, and 16.57% linoleic acid methyl. This extraction method is an environmentally benign and advantageous scheme for the production of kernel oil from Elaeagnus mollis, which will be further transformed into the value-added biomaterials and bioethanol.

Elaeagnus mollis  /  kernel oil  /  central composite design  /  response surface methodology