The recombinant inbred lines (RILs) between Ningchun 4 and Ningchun 27 are used as the donors for the construction of the spring wheat genetic linkage map by different SSR markers. It is revealed that 307 pairs of the SSR markers in total are available to show the SSR polymorphism differences between the two parents among 1001 pairs of SSR markers originally selected. The polymorphism frequency is 30.7%. The 307 pairs of the SSR primers with the polymorphisms between Ningchun 4 and Ningchun 27 are applied to further analyze the RIL population, and 266 loci with polymorphism are detected by the primers mentioned above. The χ² test (P<0.05) shows that 147 SSR markers see a significant segregation distortion of 55.3%, mostly distributed on the B and D genomes. Among them, the segregated distortions of 129 SSR markers are resulted from the female parent of Ningchun 4. By using the software of Mapmaker 3.0 and Mapdraw 2.1, 266 SSR markers are assigned on the linkage genetic map of the wheat, which covers about 2187.79 cM of the wheat genome with an average distance of 8.22 cM between markers.

spring wheat  /  recombinant inbred lines  /  SSR markers  /  linkage map