The effect of nitrogen fertilizer application rate on chlorophyll fluorescence characteristics and dry matter accumulation of switchgrass (Panicum virgatum) is of great significance for the improvement of light use efficiency and biomass yield of switchgrass in saline-alkali land. In this research, the patterns of variation in chlorophyll fluorescence parameters and dry matter accumulation in switchgrass were studied in field conditions with a range of nitrogen application treatments: no nitrogen (0 kg·ha^-1, N₀), low nitrogen application (60 kg·ha^-1, N₆₀), medium nitrogen application (120 kg·ha^-1, N₁₂₀) and high nitrogen application (240 kg·ha^-1, N₂₄₀), and the comprehensive effect of nitrogen application on chlorophyll fluorescence and dry matter accumulation in switchgrass was evaluated using a multivariate Grey relational analysis procedure. It was found that: under N₆₀, N₁₂₀ and N₂₄₀ treatments, the PSⅡ maximum photochemical efficiency (F_v/F_m), PSⅡ actual photochemical quantum efficiency (Φ_PSⅡ), potential activity (F_v/F_o), photochemical quenching (qP), non photochemical quenching (NPQ) and biomass yield were significantly higher, and the heat dissipation of quantum ratio (F_o/F_m) was significantly lower than for the N₀ treatment during the flowering and seed maturation stages of switchgrass development. During the flowering period of switchgrass, the PSⅡ potential activity (F_v/F_o) was initially increased and subsequently decreased across the range of nitrogen application rates, with a maximum observed value of 3.13 at N₁₂₀ (increased 16.26% compared with N₀). The effect of nitrogen application rate on dry matter accumulation differed between the crop growth stages. Dry matter accumulation was high during jointing and booting periods, reached its maximum at the seed filling stage, and decreased slightly thereafter. The maximum observed dry matter accumulation (378.13 g·hole^-1) occurred under the N₂₄₀ treatment during the post-flowering stage and was, respectively, 24.33%, 20.09% and 7.24% higher than that of the N₀, N₆₀ and N₁₂₀ treatments. Grey relational analysis showed that the association between the weighted correlation index and the ideal fertilization level was the highest under the N₂₄₀ treatment. In summary, the N₂₄₀ treatment in this study in the Yinbei saline-alkali area of Ningxia optimized the photochemical activity of PSⅡ and dry matter accumulation of switchgrass.

To explore the effects of drought and salt stress on germination characteristics of halophytes, this study simulated drought stress with different concentrations of PEG-6000 and four sodium salts (NaCl, Na₂SO₄, NaHCO₃, Na₂CO₃) to evaluate the effects of those treatments on the seed germination characteristics of Halogeton glomeratus. It was found the germination percentage and germination energy decreased with increasing PEG-6000 concentration, while the seedling fresh weight, dry weight, plant height and root activity initially increased and then decreased at higher PEG-6000 concentration. Cluster analysis and principal component analysis showed that the different PEG-6000 concentrations could be divided into two groups with 6% PEG-6000 as the point of separation. Fresh weight appeared to be an important parameter. In addition, germination percentage germination energy, seedling fresh weight, dry weight, plant height and root activity declined with increase in concentration of the four sodium salts. This indicated that salt stress inhibited seed germination and seedling growth, with the effects of the four tested sodium salts ranked: Na₂CO₃>NaHCO₃>NaCl>Na₂SO₄. Cluster analysis and principal component analysis showed that the sodium salt concentrations were divided into two groups with the separation points falling at 50.00 mmol·L^-1 NaHCO₃, 62.50 mmol·L^-1 Na₂SO₄, 25.00 mmol·L^-1 Na₂CO₃ and 100.00 mmol·L^-1 NaCl. Among the plant traits evaluated as indicators of H. glomeratus stress tolerance in the germination stage, the germination index was the key indicator of NaHCO₃ and Na₂SO₄ stress, root activity emerged as the primary indicator of Na₂CO₃ stress and dry weight was the major indicator of NaCl stress in H. glomeratus.

The purpose of this study was to evaluate the rumen degradation characteristics of conventional roughage (corncob, soybean pod shell) and unconventional roughage (corn silage, alfalfa hay) for dairy cows. Three Holstein cows with permanent ruminal cannulas were used to evaluate the ruminal degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) using the nylon-bag technique. It was found that: 1) The CP content in alfalfa hay (17.55%) was significantly higher than that in soybean pod shell (11.10%; P<0.05). The CP content in corncob (2.97%) was significantly lower than that in corn silage (8.41%; P<0.05). The NDF and ADF content in corncob (79.98% and 43.35%, respectively) were significantly higher than in the other three feedstuffs (P<0.05). 2) The DM effective degradability of alfalfa hay was the highest (P<0.05) among the tested feedstuffs, which ranked: alfalfa hay>soybean pod shell>corn silage>corncob. The CP effective degradability of soybean pod shell was significantly higher than the other three feedstuffs (P<0.05), which ranked: soybean pod shell>alfalfa hay>corn silage>corncob. The NDF effective degradability of alfalfa hay was significantly higher than soybean pod shell (P<0.05); there was no significant difference in NDF digestibility between corn silage and soybean pod shell (P<0.05). The ADF effective degradability of corncob was the highest (P<0.05) among the tested feedstuffs; there was no significant difference in ADF effective degradability between alfalfa hay, corn silage and soybean pod shell. In conclusion, corncob and soybean pod shell can be used as unconventional forage resources.

Petrobia harti is a very serious worldwide pest mite harming Oxalis corymbosa, and pyridaben as a broad-spectrum, contact-killing acaricide that is highly effective against P. harti. To clarify the sublethal effects of pyridaben on P. harti and provide data for developing comprehensive control strategies for this mite with minimal usage of pyridaben, the toxicity of pyridaben against P. harti was determined by a leaf impregnation method. The sublethal doses used were determined by a regression equation of P. harti death rate on pyridaben concentration, in order to identify concentrations predicted to be lethal to 10% or 20% of a test population of mites (LC₁₀ and LC₂₀, respectively). The effects of the LC₁₀ and LC₂₀ sublethal pyridaben doses on the development, fertility and life-history parameters of P. harti were evaluated by establishing a life table. It was found that the fecundity of P. harti was significantly decreased after treatment with the sublethal doses of pyridaben (P<0.05) with the number of eggs laid per female reduced, respectively, by 53.37% and 55.46% in the F₀ generation and 41.34% and 45.24% in the F₁ generation in the LC₁₀ and LC₂₀ treatments, compared to the controls. Similarly, the oviposition duration was significantly shortened, respectively, by 3.11 and 4.75 days in F₀ generation and 3.47 and 2.81 days in the F₁ generation. The average adult longevity was also significantly shortened, respectively, by 1.45 and 2.04 days in the F₀ generation and 3.24 and 4.00 days in the F₁ generation. Compared to the control, after treatment with sublethal pyridaben doses, the net reproductive rate (R₀), intrinsic rate of increase (r_m) and finite rate of increase (λ) of P. harti were all significantly decreased in the LC₁₀ and LC₂₀ treatments (the R₀ from 28.54 to 16.91 and 15.48; the r_m from 0.1650 to 0.1276 and 0.1249; the λ from 1.17941 to 1.1435 and 1.1330; P<0.05), while the mean generation time (T) and the population doubling time (Dt) were both significantly prolonged (the T from 20.31 to 22.17 and 21.94 days; the Dt from 4.20 to 5.43 and 5.55 days, respectively; P<0.05). The results indicated that the sublethal doses of pyridaben at LC₁₀ and LC₂₀ decreased the development rate and fecundity of laboratory populations of P. harti.

Understanding of the transformations and recycling processes of carbon, nitrogen and phosphorus in the soil rhizosphere is fundamental to solving the problems currently limiting the sustainable utilization of grassland agricultural ecosystems and improving grassland productivity. Rhizosphere processes in grassland can reflect the soil carbon, nitrogen and phosphorus nutrient turnover rates, affect the competition for and capture of nutrients by plants and soil microorganisms, and also determine the nutrient balance of each component in the rhizosphere microenvironment. Compared with crop systems, the coupling between carbon, nitrogen and phosphorus in grassland is stronger. Therefore, the structure and function of rhizosphere micro-ecosystems play an important role in maintaining nutrient transformation and circulation. Many literature reports indicate that the critical factor that regulates the rhizosphere microenvironment is the key substance of the plant and soil for material exchange and information transmission. The root exudates and rhizosphere exudates are also the key substances that mediate rhizosphere microorganisms and enzyme activities. When the grassland environment changes, the rhizosphere exudates, rhizosphere enzyme activities, and the composition and diversity of rhizosphere soil microorganisms will also change fundamentally, which will cause a change of supply and availability of soil nutrients, and in turn will affect grassland productivity and utilization efficiency of nutrients. So, further research on the regulation mechanism of nutrient cycling in grassland rhizosphere process is very important for revealing the distribution and utilization of nutrients and nutrient exchanges between grassland plants and microorganisms. This article reviews the mechanisms and interactions involving rhizosphere exudates, rhizosphere enzymes, and rhizosphere microorganisms involved in nutrient cycling, summarizes the mechanisms of grassland rhizosphere processes contributing to nutrient cycling and provides a perspective for consideration when formulating future research on rhizosphere microdomains in grassland ecosystems.

This research explored the physiological leaf response mechanisms of sugar beet to re-watering after drought with a focus on photosynthetic physiological traits and photo-responsive parameters. Two cultivars with different drought resistance, ‘XJT9907’ (a drought sensitive type) and ‘XJT9916’ (a drought tolerant type) were studied. A controlled water deficit was imposed on plants in the leafy growth stage, whereby soil moisture content was allowed to fall to 45%-50% of field water holding capacity for 7 days, then irrigation water was added to raise soil moisture to 70%-75% of the field water holding capacity for 48 hours. At this point, the photosynthetic physiological parameters of leaves were determined, and nonlinear regression used to fit sigmoid curves for the relationship between net photosynthetic rate of leaves and light intensity. It was found that drought stress during the leafy growth stage of sugar beet development significantly reduced the SPAD value, net photosynthetic rate (P_n), stomatal conductance (G_s), intercellular CO₂ concentration (C_i), and transpiration rate (T_r) of both sugar beet varieties. After re-watering, the SPAD value, net photosynthetic rate (P_n), stomatal conductance (G_s), intercellular CO₂ concentration (C_i) and transpiration rate (T_r) of sugar beet leaves under drought stress treatments all improved. The values of physiological parameters for drought tolerant XJT9916 were significantly higher than those of drought sensitive XJT9907, although none of the values exceeded those of plants with a normal level of water supply, indicating a degree of compensation effect. After the re-watering treatment, the maximum net photosynthetic rates (P_nmax) of XJT9916 and XJT9907 varieties were, respectively, 6.2% and 17.1% lower than the control, and the apparent quantum efficiency (AQY) was, respectively, 6.5% and 12.2% lower than the control. Similarly, comparing drought tolerant and drought sensitive varieties to control plants. respiration rates (Rd) were, respectively, 19.1% and 14.9% lower than the control, light saturation points (LSP) were 19.6% and 14.1% lower than the control, while the light compensation points (LCP) were, respectively, 15.4% and 17.6% (P<0.05) higher than the control. For the drought sensitive variety, the light energy utilization interval was comparatively narrow, and the light energy utilization efficiency was reduced. Taken together, the results show that when the soil moisture content is 45%-50% of the field water holding capacity during the sugar beet leafy growth period, the photosynthetic potential of sugar beet leaves is not realized, the photosynthetic capacity of the leaves is weakened and cannot be restored to normal levels after re-watering. The drought-tolerant sugar beet variety XJT9916 has strong photosynthetic recovery ability in the context of re-watering after drought.

Grassland is an important natural resource and plays a key role in maintaining the stability of ecosystems on a regional scale. Confirmation of grassland contracts and property rights provides an institutional guarantee for grassland ecological protection and development, and is a specific measure to implement the relevant central government requirements. Previous research showed that the speed of determining grassland ownership varies from region to region due to unique local factors in the various grassland areas in China, and in some regions the progress towards determining the ownership of grasslands is relatively slow. Based on analysis of the historical background and current situations relating to contract and property confirmation for grasslands, we here summarize the basic work in grassland contract and property confirmation, including data collection, contract content, contract optimization, issuing of certificates of ownership, landholder training, information management and improvement of grassland dispute resolution mechanisms, et al. The thinking of the planning sector in grassland management including the main building blocks, basic function, workflow, key technologies, is put forward. Finally, consideration of and suggestions for the next stage of the work involving ‘three property rights separation’ are put forward. This research is of great significance to furthering the development of the contract and information management of grasslands in China.

This study investigated the relationship between soil moisture and vegetation cover in alpine steppe meadow in the Qilian Mountains with the aim of improving the precision of remote sensing estimates of soil moisture and vegetation cover. Six sample areas with different stocking rates of Cervus elaphus kansuensis (1.00, 1.45, 2.45, 3.45, 4.85, 6.90 AUM·ha^-1) in were set up, AUM means animal unit. Through comparing data from monthly field measurement of vegetation cover and soil moisture and SPOT-TM images combining Landsat5 multispectral images and SPOT2 panchromatic images, we analyzed the relationship between soil moisture, vegetation cover and normalized difference vegetation index (NDVI) and quantitatively explored the dynamics of seasonal change in vegetation cover and soil moisture in the sample areas in the Qilian Mountain alpine steppe. Except for Achnatherum inebrians, Convolvulus ammannii and Stragalus leucocephalus, the response of population cover to surface soil moisture was relatively sensitive, with the population cover of species inversely proportional to soil moisture in the range between c. 5% and c. 25%. The response of plant community cover to surface soil moisture was also relatively sensitive. In the soil moisture range from 10.0% to 1.1%, the community cover increased in the inverse proportional function. The results are highly relevant to remote sensing monitoring of the drought resistance and water use efficiency of plant species, populations and communities in arid and semi-arid steppe grassland, and provide scientific data for vegetation and soil moisture management in steppe grasslands.

This research investigated the effect of lactic acid bacteria (LAB) preparations on microbial population counts and silage quality of whole maize silage during fermentation and on aerobic exposure after fermentation. The aim was to test if inoculation with LAB preparations can improve silage quality and aerobic stability. Three different LAB preparations [Homofermentative (Homo), Heterofermentative (Hetero), combined Homo+Hetero] were added to whole maize during ensiling. A blank control treatment was also included. Data on microbial population count, fermentation quality and nutrient contents of silage were evaluated during fermentation at 3, 7, 15, and 30 days after ensiling, and on completion of fermentation, after 1, 3, 7, 15 and 30 days of aerobic exposure. It was found that both during maize silage fermentation and during subsequent aerobic exposure, the LAB population counts, the lactic and acetic acid concentrations were significantly increased compared with the blank control treatment, while the numbers of aerobic bacteria, yeasts and molds and crude protein loss were significantly reduced and the concentrations of NH₃-N and the pH were obviously reduced by addition of the three LAB preparations. These results indicate that LAB preparations improve maize silage nutritional quality and inhibit secondary fermentation after aerobic exposure. The combination treatment Homo+Hetero performed best, and Homo performed better than Hetero.

This research investigated seed germination and seedling growth of four species of bioenergy grass (Pennisetum americanum×Pennisetum purpureum, Pennisetum purpureum, Pennisetum hydridum and Sorghum sudanense), grown on electrolytic manganese residue (control) or electrolytic manganese residue after adding one of three amendments (5% diatomite, 5% charcoal or 2.5% charcoal+2.5% diatomite). Germination percentage for the four bioenergy grasses ranked: P. purpureum>P. americanum×P. purpureum>S. sudanense>P. hydridum. The three amendments all significantly improved the germination percentage, germination energy and germination index of P. hydridum, and for the other three grasses the germination energy and germination index in the mixed amendment treatment of diatomite and charcoal were also significantly higher than those of the control group (P<0.05). Compared with the control group, in the amendment treatments, the fresh weights of P. americanum×P. purpureum, P. purpureum, P. hydridum and S. sudanense were increased by 36.54%, 7.69%, 35.71% and 23.88%, respectively. Amendment application also enhanced chlorophyll content and the activities of antioxidant enzymes (superoxide dismutase, peroxidase, catalase), while the malondialdehyde content of leaves was decreased. Based on the germination, growth and physiological parameters, for the tested bioenergy grasses grown on the electrolytic manganese residue, P. purpureum with 2.5% charcoal and 2.5% diatomite amendment performed best, followed by P. americanum×P. purpureum with 5% diatomite amendment.