PDF
Full
Outline
OBJECTIVE To prepare bimodal mesoporous silica nanospheres loaded with doxorubicin, and evaluate their adsorption/ desorption properties and proliferation inhibition effects on cancer cells. METHODS bimodal mesoporous silica nanospheres(BMSNs) were prepared using sol-gel method and thermal treatment. The nanospheres were analyzed and characterized using scanning electron microscopy, specific surface area and porosity analyzer, Fourier transform infrared spectroscopy, and UV-VIS spectrometer. The adsorption/desorption characteristics of DOX by BMSNs were investigated by adsorption experiment. The proliferation inhibition effects of BMSNs-DOX on MCF-7 cells and A549 cells were detected using CCK8 method. RESULTS Compared with mesoporous MCM-41, which has typical uniform mesopores, BMSNs could provide more favorable matter diffusion and adsorption capacity towards DOX due to their more expansive pore structure. At pH=7.0, BMSNs exhibited a high adsorption capacity for DOX of 91.7 μmol·g-1 (equivalent to a drug loading capacity of 5.32%), which was 1.6 times higher than that of MCM-41. In pH=5.0 buffer solution, BMSNs-DOX desorbed (released) DOX molecules with an efficiency of about 96%, in just 30 minutes. BMSNs-DOX exhibited a stronger inhibitory effect than MCM-41-DOX and DOX. At a concentration of 4 μg·mL-1, the survival rate of MCF-7 cells and A549 cells was only 23.67% and 15.79% respectively. CONCLUSION Compared with MCM-41, BMSNs is expected to release DOX to cancer cells more efficiently. BMSNs-DOX exhibits a significant inhibitory effect on MCF-7 cells and A549 cells, suggesting its potential as a drug carrier to enhance the cytotoxicity of chemotherapy drugs on cancer cells.
PDF
131
61
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
