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OBJECTIVE To prepare bimodal mesoporous silica nanospheres loaded with doxorubicin, and evaluate their adsorption/ desorption properties and proliferation inhibition effects on cancer cells. METHODS bimodal mesoporous silica nanospheres(BMSNs) were prepared using sol-gel method and thermal treatment. The nanospheres were analyzed and characterized using scanning electron microscopy, specific surface area and porosity analyzer, Fourier transform infrared spectroscopy, and UV-VIS spectrometer. The adsorption/desorption characteristics of DOX by BMSNs were investigated by adsorption experiment. The proliferation inhibition effects of BMSNs-DOX on MCF-7 cells and A549 cells were detected using CCK8 method. RESULTS Compared with mesoporous MCM-41, which has typical uniform mesopores, BMSNs could provide more favorable matter diffusion and adsorption capacity towards DOX due to their more expansive pore structure. At pH=7.0, BMSNs exhibited a high adsorption capacity for DOX of 91.7 μmol·g-1 (equivalent to a drug loading capacity of 5.32%), which was 1.6 times higher than that of MCM-41. In pH=5.0 buffer solution, BMSNs-DOX desorbed (released) DOX molecules with an efficiency of about 96%, in just 30 minutes. BMSNs-DOX exhibited a stronger inhibitory effect than MCM-41-DOX and DOX. At a concentration of 4 μg·mL-1, the survival rate of MCF-7 cells and A549 cells was only 23.67% and 15.79% respectively. CONCLUSION Compared with MCM-41, BMSNs is expected to release DOX to cancer cells more efficiently. BMSNs-DOX exhibits a significant inhibitory effect on MCF-7 cells and A549 cells, suggesting its potential as a drug carrier to enhance the cytotoxicity of chemotherapy drugs on cancer cells.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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