Full
Outline
To evaluate the anti-inflammatory and analgesic effects of Naizilai Granules (NZL) and to preliminarily investigate the underlying mechanisms.
The anti-inflammatory and analgesic effects of NZL were evaluated in mice using the acetic acid-induced writhing test as well as the egg white-induced paw edema model. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-12 (IL-12), and interleukin-1 beta (IL-1β) were quantified by enzyme-linked immunosorbent assay (ELISA). In lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, the inhibitory effect of NZL on inflammation was assessed. Additionally, the cytokine levels (IL-12, IL-1β and TNF-α) in cell supernatants were measured, and the protein expression of cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) was detected by Western blot (WB).
The acetic acid-induced writhing test demonstrated a significant reduction in the number of writhes in NZL-treat groups compared to the model group. In the egg white-induced paw edema model, NZL treatment groups showed markedly decreased paw swelling compared to the model group. Furthermore, NZL effectively inhibited the LPS-induced inflammation in RAW264.7 cells and decreased the expression levels of IL-12, IL-1β, and TNF-α in cell supernatants. Additionally, there was a marked reduction in the expression of COX-2 and PGE2.
NZL exhibits analgesic and anti-inflammatory effects.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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