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Simultaneous determination of multiple constituents of QingJinHuaTan Decoction by ultra high performance liquid chromatography
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Jing LIU, Ran LIU, Dan-dan LI, Hua-rong XU, Qing LI, Kai-shun BI*
Acta Pharmaceutica Sinica | 2020, 55(8) : 1872 - 1876
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Acta Pharmaceutica Sinica | 2020, 55(8): 1872-1876
Simultaneous determination of multiple constituents of QingJinHuaTan Decoction by ultra high performance liquid chromatography
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Jing LIU, Ran LIU, Dan-dan LI, Hua-rong XU, Qing LI, Kai-shun BI*
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Published: 2020-08-12 doi: 10.16438/j.0513-4870.2020-0010
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A UHPLC method for the simultaneous determination of multiple constituents in QingJinHuaTan Decoction was established. The separation was performed on a Waters cortecs T3 column (150 mm×2.1 mm, 1.6 μm); the mobile phase was acetonitrile-water (containing 0.04% phosphoric acid) with gradient elution at a flow rate of 0.30 mL·min-1, the column temperature at 25℃ and the wavelengths at 238 nm and 280 nm. The results showed that all peaks were well separated and all components had a good linear relationship in the investigative range, (r > 0.999). The repeatability and stability were good and the recovery was between 92.5%-104.7%. The method is simple, accurate and reliable and provides a basis for quality control of QingJinHuaTan Decoction and for further development of methods for its standardization.

classical prescription  /  QingJinHuaTan Decoction  /  UHPLC  /  content determination  /  quality control
Jing LIU, Ran LIU, Dan-dan LI, Hua-rong XU, Qing LI, Kai-shun BI. Simultaneous determination of multiple constituents of QingJinHuaTan Decoction by ultra high performance liquid chromatography[J]. Acta Pharmaceutica Sinica, 2020 , 55 (8) : 1872 -1876 . DOI: 10.16438/j.0513-4870.2020-0010
Year 2020 volume 55 Issue 8
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doi: 10.16438/j.0513-4870.2020-0010
  • Receive Date:2020-01-02
  • Online Date:2026-01-21
  • Published:2020-08-12
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  • Received:2020-01-02
  • Revised:2020-03-27
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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