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To establish the substances benchmarks fingerprint of Daqinglong decoction and determine the content and transfer rate of 6 index components，and study the transfer rule of Daqinglong decoction substances benchmarks value，which laid the foundation for the study of Daqinglong decoction compound preparation and quality standard.
Fifteen batches of substances benchmarks of Daqinglong decoction were prepared，and the paste rate was determined. To determine the contents of ephedrine，pseudoephedrine，amygdalin，and cinnamic acid，HPLC method was performed on a Dikma Platisil C18 column (250 mm×4.6 mm，5 μm). And 0.15% phosphoric acid water (A) and acetonitrile (B) were used as mobile phases with gradient elution at a flow rate of 1 mL·min-1. The column temperature was 25 ℃，the detection wavelength was 207 nm，and the injection volume was 10 μL. To determine the contents of liquiritin and glycyrrhizic acid，0.05% phosphoric acid water (A) and acetonitrile (B) were used as mobile phases with gradient elution. The detection wavelength was 237 nm. The transfer rate was calculated，and the transfer analysis from the decoction pieces to the substances benchmarks was carried out.
The fingerprint similarities of 15 batches of Daqinglong decoction substances were ≥0.921. Fifteen common peaks were identified，including 8 from Ephedrae Herba，3 from Cinnamomi Ramulus，2 from Glycyrrhizae Radix et Rhizoma(fried)，and 2 from Armeniacae Semen Amarum(here). The contents of ephedrine，pseudoephedrine，amygdalin，liquiritin，cinnamic acid and glycyrrhizic acid were 0.94%-1.30%，0.56%-1.02%，0.70%-1.25%，0.18%-0.32%，0.05%-0.10% and 0.46%-1.23%，respectively. The transfer rates were 41.67%-59.47%，42.66%-59.74%，17.59%-36.34%，21.48%-44.75%，31.06%-48.89% and 12.95%-25.15%，respectively. The paste yields of substances benchmarks were 11.98%-13.38%.
The fingerprint combined with the determination of paste rates and index component contents are used to study the quantitative value transfer of Daqinglong decoction substance benchmarks，and initially establish a scientific and stable substance benchmarks quality evaluation method，which can provide reference for the future research on compound preparation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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