Full
Outline
Intravital two-photon imaging technology has become an essential tool in life science research due to its ability to intuitively monitor the in vivo dynamic processes of living organisms. However, sample preparation has always been a major challenge in the application of this technology, and it directly determines the stability and quality of imaging. There is currently no systematic introduction to sample preparation for intravital two-photon imaging. Based on the rich practical experience accumulated in the laboratory over the years in the preparation of samples for intravital imaging, we mainly introduce the sample preparation procedures and key points of operation for tissues such as the lung, intestine, brain, liver, and skin. The aim is to provide a reference for sample preparation and operation before intravital two-photon imaging, assist researchers in mastering the operation of intravital two-photon imaging, and promote the broader application of this technology.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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