ABSTRACT: The management and disposal of laboratory waste is of great significance to environmental protection. This article classifies and analyzes laboratory waste, pointing out that it is mainly divided into chemical waste, biological waste and radioactive waste, and explores the potential hazards of different types of waste. This paper proposes a series of environmental protection strategies, including waste classification management, collection and temporary storage, treatment technology, waste transportation and final disposal, reduction strategies, environmental monitoring and assessment, and personnel training management. Through the implementation of effective classification and management, the use of advanced treatment technology, and the reduction of waste generation, the harm of laboratory waste to the environment can be minimized, and the sustainable treatment and environmental protection of laboratory waste can be promoted.

In recent years, the number of inspection and testing institutions in China has continued to grow, and the industry's overall revenue has also increased significantly. However, as market competition intensifies, inspection and testing institutions face dual challenges of cost and efficiency. This article took Centre Testing International Group Co., Ltd. as an example to introduce the concept of lean management in the laboratory, including the use of standardization models, electronic laboratory notebooks, and robot automation tools, pointed out that lean management optimizes laboratory workflow and resource allocation, improves efficiency and reduces cost, and discussed the potential advantages and promotion prospects of lean management in the field of inspection and testing, providing reference and exemplar for relevant inspection and testing institutions.

Objective To evaluate the advantage clinical value of cold digestion method and national standard method in the determination of serum iodine. Methods The DAT-500 iodine detector of Changsha Seclude and the cold digested serum iodine detection reagent (patent: CN113340888A reagent kit and detection method for serum quantitative detection) were used to compare with the national standard Determination of Iodine in Serum by Catalytic Spectrophotometry. Results From 0 to${300\mu }\mathrm{g}/\mathrm{L}$iodine content range, the absolute value of correlation coefficient of standard curve of 5 batches exceeded 0.9995, the detection limit was${2.1\mu }\mathrm{g}/\mathrm{L}$; the recovery rate of 9 serum samples with different concentrations was${94.6}\%\sim {108.8}\%;6$batches of 3 serum samples with different concentrations were tested, the RSD was${3.41}\%\sim {4.01}\%$, and the recovery rate was${94.6}\%\sim {108.8}\%$; there was no statistical significance between 20 serum samples and national standard method$\left({t =- {1.660}, P ={0.113}> {0.05}}\right)$. Conclusion Cold digestion method has the advantages of high accuracy and accuracy, simple operation, short time consuming, low demand for temperature control, easy storage and transportation at room temperature.

Objective In order to clarify the pollution situation of heavy metal elements in water basins and improve the detection accuracy of heavy metal elements, a study on the detection of heavy metal elements in water basins based on inductively coupled plasma mass spectrometry (ICP-MS) method is conducted. Methods By selecting appropriate detection chemical reagents, ensure the accuracy and sensitivity of the detection process. The preparation of chromatographic columns suitable for ICP-MS method provides strong support for the determination of heavy metal elements. To ensure the reliability of the test results, the collection and pretreatment process of water basin samples has been strictly standardized. In the process of chromatographic column screening and heavy metal element determination, the optimal chromatographic column is selected for quantitative determination of heavy metal elements. Results The detection method based on ICP-MS has high detection accuracy for heavy metal elements in water basins, with small errors compared to actual situations. Conclusion This provides strong technical support for monitoring heavy metal pollution in water basins.

Objective In order to ensure the quality control of enterprise production products and improve detection efficiency, a high-performance liquid chromatography method for Benzyl Propionate Oxadiazon Granules is established. Methods The HPLC method was used to separate and determine the contents of Benzyl Propionate Oxadiazon Granules which contained the active ingredients mentioned above. In the method, the column ZORBAX Eclipse Plus${\mathrm{C}}_{18}$(${4.6}\mathrm{\;{mm}}\times {50}\mathrm{\;{mm}},{1.8}\mathrm{\;{\mu m}},{600}\mathrm{\;{Bar}}$) was used and the mixture of acetonitrile, isopropanol and 0.05% phosphoric acid solution was used as the mobile phase, and${245}\mathrm{\;{nm}}$was the selected wavelength in the determination. Results It has been found that bensulfuron methyl, pretilachlor, and oxadiazon not only have high chromatographic peak purity under this analytical method, but also have a good linear relationship. The peak purity of bensulfuron methyl in this product is 992, with a recovery rate of 98.82%. The peak purity of pretilachlor is 993.3, with a recovery rate of 99.88%. The peak purity of oxadiazon is 999.3, and the recovery rate is 99.22%. Conclusion This chromatographic method is stable and suitable for quality control analysis of this product.

Unpredictability and great harm are the remarkable characteristics of sudden public health events. If the event occurs, it will certainly have an impact on the physical and mental health of the broad masses of the people, and will also harm the social stability. Public health emergencies mainly involve food and drugs. By enhancing the quality control of physical and chemical testing, the factors that cause the formation of public health emergencies can be found as soon as possible. Therefore, relevant staff need to pay more attention to this aspect. This paper will from the centers for disease control of public health emergencies in the quality control strategy, first analysis the significance of the control of physical and chemical inspection quality, then explore the factors affecting the physical and chemical test results, finally study the effective method to strengthen the inspection quality control, for the public health as a sudden to give important guarantee as soon as possible.

Objective To study the method of laboratory Brucella detection and evaluate its clinical efficacy. Methods The clinical case of Brucella infection in sheep diagnosed by medical staff in our hospital in March 2019 was taken as the research object. The advantages and disadvantages of serum agglutination test, routine blood culture and bacterial identification, and mass spectrometry identification methods in diagnosing Brucella were analyzed and discussed, providing some reference for the detection of Brucella in grassroots hospital laboratories. Results The medical staff's serum brucellosis tiger red plate agglutination antigen test was positive. The blood culture BD PhoenixTM whole bacterial identification/drug sensitivity system instrument in the United States indicates that the aerobic bottle reports positive, but cannot identify the species of Brucella. The mass spectrometer identified it as Brucella lamblia. Conclusion The above methods have their own advantages and disadvantages in application, grassroots hospital laboratories should choose suitable methods for detecting Brucella based on actual conditions.

Since the implementation of the State administration for market supervision and administration's Measures for the Administration of the Qualification of Inspection and Testing Institutions (Order No. 163) amendment and Measures for the Supervision and Administration of Inspection and Testing Institutions (Order No. 39) in 2021, regulatory authorities at all levels have significantly intensified their efforts in conducting random inspections and targeted rectification campaigns. Cases of inspection and testing agencies being stripped of their qualifications, suspended, fined, or publicly reprimanded have frequently appeared in the media, attracting significant attention from the industry. As inspection and testing agencies, it is imperative to enhance their quality compliance management capabilities, thoroughly study and understand various regulatory policies and cases, and strengthen internal quality compliance management to mitigate the risk of administrative penalties, thereby boosting the confidence of all sectors of society in the industry. This article first introduces the necessity and urgency of introducing a quality risk management mechanism in laboratories. It then describes various sources of risk. Finally, it elaborates on the planning, implementation, performance evaluation, and improvement of the quality compliance risk management mechanism in laboratories, aiming to help readers initially grasp and understand the basic approach to implementing quality compliance risk management in laboratories.

Objective To explore the regulatory effect of hypericin targeting Nrf2/HO-1 pathway on pancreatic beta cell function in Type 2 diabetes and its potential molecular mechanism. Methods Induced oxidative damage in rat pancreatic beta cell line RIN-m5F by hydrogen peroxide$\left({{\mathrm{H}}_{2}{\mathrm{O}}_{2}}\right)$, pancreatic beta cells were treated with 0,25,50, and 100$\mu$M hyperoside and ML385 (Nrf2 inhibitor). Real time fluorescence quantitative PCR (RT-qPCR) was used to evaluate Nrf2 and HO-1, protein imprinting was used to detect cleaved-Caspase3 and Caspase3, and glucose stimulated insulin secretion assay, 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyltetrazolium bromide thiazole blue, tetrazolium tetrazolium salt (MTT), flow cytometry, and oxidative stress assay kit were used to detect insulin, cell viability, apoptosis, and oxidative stress in rat pancreatic beta cell line RIN-m5F. Results A certain concentration of hyperoside had no cytotoxic effect on RIN-m5F pancreatic beta cells and increased cell survival rate. Hypericin dose dependently reduced apoptosis rate, reactive oxygen species (ROS), and malondialdehyde (MDA) levels, while increasing catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and insulin levels. With the dose-dependent increase of hyperoside, the level of cleaved-Caspase3 significantly decreased, the ratio of cleaved-Caspase3/Caspase3 significantly decreased, and the expression levels of Nrf2 and HO-1 significantly increased. After the addition of Nrf2 inhibitor ML385, the overall expression level decreased, with the same trend as before. Hypericin can activate the levels of antioxidant response elements (ARE) and heat shock protein (HSP90 α), indicating that it can initiate the transcription of a series of antioxidant genes and regulate antioxidant responses. Conclusion Hyperin plays a protective role in Type 2 diabetes islet beta cells by activating Nrf2/HO-1/ARE pathway, and improves the function of islet beta cells.

Against the backdrop of continuous socio-economic progress in China, the public has set higher standards and requirements for the quality and safety of food. In view of this, food safety issues have gradually become a focus of social attention. Given the diversity and vast quantity of food in the market, consumers often find it difficult to accurately assess its safety when selecting food. Therefore, inspection agencies must increase investment in food safety inspections and use advanced testing techniques to ensure the effective protection of China's food safety level. In this regard, this article first outlines rapid detection technology, analyzes the application significance of food safety rapid detection technology, explores the key points of rapid detection technology in food safety testing, and proposes measures to promote the application of food safety rapid detection technology for reference.