Objective Through the statistical analysis of the actual detection results of Down's disease screening and the pregnancy outcome of follow-up in Zhuhai, the health economics evaluation was carried out. Methods The data of 31967 pregnant women who underwent serum screening (SS) and non-invasive prenatal testing (NIPT) during the same pregnancy in Zhuhai City from 2018 to 2021 were retrospectively analyzed. The cost-benefit analysis was conducted according to 5 different screening strategies. Results Among 31,967 pregnancies, 38 were Down syndrome babies (including 3 pregnant women aged over 35 years), with a Down syndrome incidence of 1/841. Serological screening detected 1,649 cases of high risk of T21, of which 21 were true positives, with a positive predictive value of 1.27% and a detection rate of 55.3%; NIPT screening detected 49 cases of high risk of T21, 38 cases of Down syndrome babies, with a positive predictive value of 77.55% and a detection rate of 100%. The total cost generated by calculation strategy 4 was the lowest, about 27.44 million yuan. Conclusion NIPT can be used as primary screening test instead of serological screening method in prenatal Down's screening for secondary prevention and control of birth defects, which has great social and economic benefits.

Objective This study aims to evaluate the stability and repeatability of digital pressure gauges in laboratory environments, to verify their measurement accuracy in different pressure ranges and performance in long-term use, and to provide scientific basis for the calibration and application of digital pressure gauges. Methods The experiment adopted a multi-point and multiple measurement method, selecting three different pressure points of 0.1,0.3,0.6 MPa for testing. Ten repeated measurements were taken at each pressure point to evaluate the repeatability of the digital pressure gauge. Meanwhile, through long-term measurements every three months for one year, observe the stability performance of the digital pressure gauge during long-term use. The experimental environment conditions are strictly controlled, with a temperature maintained at${\left({20}\pm 2\right)}^{\circ}\mathrm{C}$and a relative humidity between${40}\%$and${60}\%$. Results The standard deviations of the digital pressure gauge at the three pressure points were 0.10088 , 0.30057 and 0.60087, respectively. The standard deviations were small and the repeatability was good. At the same time, during long-term use, the measurement values at each measurement point have a relatively small change amplitude, and the changes in range and average values are within the allowable error range, indicating that it has good long-term stability. Conclusion Digital pressure gauges exhibit high repeatability and stability in laboratory environments, making them suitable for high-precision pressure measurement applications. This study provides a scientific basis for the calibration and practical application of digital pressure gauges, and also provides a reference direction for the research and improvement of related equipment in the future.

With the rapid development of medical science, laboratories in medical departments of higher education institutions have become the core areas for innovation and teaching. The precise research equipment in the laboratory is the cornerstone for conducting cutting-edge research and medical experimental teaching. However, the complexity, high value, and rapid iterative updates of laboratory equipment require a more refined management model to ensure optimal instrument performance and extend its lifespan. In the face of increasingly fierce international competition, efficient management of laboratory instruments is particularly important: it directly affects the progress and quality of laboratory research, as well as the academic status and educational quality of the entire department.

Objective The structure and composition of Shoushan tianhuang were determined, and in order to understand the reason why field-yellow stone is different from other varieties of koreite. Methods It’s structure was observed by microscope and scanning electron microscope, and it’s chemical composition was analyzed by near infrared spectrometer, X-ray powder diffractometer and ICP-MS. Results By scanning electron microscopy, it was found that the crystal structure of fShoushan tianhuang is aphanocrystalline, the crystal is lamellar, the particle size is uneven, the overall orientation is poor, there is idiomorphic or hemidiomorphic pyrite and the particle boundary is clear. Comparing and analyzing the data, it is found that$\mathrm{{Cr}}$element is stable and there are three kinds of mineral composition in Shoushan tianhuang: illite is the main mineral. The main mineral is the dickite and the secondary mineral is the illite. The main minerals are dickite and kaolinite. Conclusion The analysis of structure and chemical composition of Shoushan tianhuang is of great value for identifying varieties of koreite and distinguishing different mining areas, and also provides important data support for understanding the production state and particularity of Shoushan tianhuang.

With the increasingly serious environmental pollution, food may be affected by various new pollutants, such as microplastics and heavy metals. Therefore, it is necessary to continuously study the detection methods of new pollutants to ensure the overall safety of food. This article makes a deep thinking and innovative discussion on food testing work under the background of food safety.

This study aims to develop a rapid detection method based on multiplex real-time quantitative PCR technology for accurate identification and quantification of common pathogenic bacteria on face masks, to assess the hygienic condition of masks and control nosocomial infections. Methods Using TaqMan probe-based real-time fluorescence quantitative PCR as the core technology, specific primers and fluorescently labeled probes were designed and optimized for simultaneous quantification of specific gene sequences from three pathogens through one-tube multiplex detection. The experiment began by screening specific target genes and sequences from the NCBI database and designing primers and probes. Subsequently, quality testing of primers and probes was conducted, and standard samples were prepared through amplification of specific sequences and TA cloning. Finally, standard curves were established, and mask samples were tested. Results After optimizing experimental conditions, an efficient and stable multiplex real-time quantitative PCR detection system was successfully established. The detection efficiency of standard samples for three pathogens was evaluated at different concentration gradients. The detection data showed that the primers and probes had high specificity for target pathogens, and the amplification efficiency and signal intensity met experimental requirements. In the detection of mask samples, the method accurately quantified the genomic DNA copy numbers of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus hemolyticus, thereby assessing the degree of microbial contamination on masks. Conclusion The rapid detection method for pathogenic bacteria on masks based on multiplex real-time quantitative PCR technology demonstrates high sensitivity, specificity, and speed, providing strong technical support for clinical and public health

Objective To study error compensation techniques in high-precision pressure detection systems and improve measurement accuracy. Methods A comprehensive error compensation method based on neural networks was proposed, and a pressure sensor error model was established. An adaptive neural network compensator was designed. Results This method achieves effective compensation for nonlinear errors, and experimental results show that the systematic nonlinear error decreases from 0.25% FS to 0.015% FS and the maximum relative error from 0.38% to 0.022%. After temperature compensation, the temperature coefficient decreased to${0.002}\%\mathrm{{FS}}/{}^{\circ}\mathrm{C}$, which increased by${80}\%$compared to$\pm {0.01}\%$FS/${}^{\circ}\mathrm{C}$before compensation. The dynamic response time is in the${1.7}\sim {2.0}\mathrm{\;{ms}}$range, with a$-3\mathrm{\;{dB}}$bandwidth of${850}\sim {870}\mathrm{\;{Hz}}$. Conclusion The error compensation method proposed in this paper can effectively improve the measurement accuracy of pressure detection systems and provide new ideas for the development of high-precision pressure detection technology.

In order to achieve real-time monitoring of the status of fire-fighting equipment, improve detection efficiency and real-time data, this paper mainly studies the remote detection of fire-fighting products using advanced network data collection and security technologies. The remote detection system for fire protection products faces security risks in data transmission and storage, including data theft, tampering, and network attacks. It must apply encryption technology, identity authentication, multi factor authentication, firewalls, and intrusion detection systems. In addition, it is necessary to comply with data privacy regulations and perform data anonymization and de identification processing to protect user privacy, which is of great significance for achieving remote detection of fire protection products.

Objective To observe the test results and substantial impact of real-time fluorescence quantitative PCR detection technology in detecting hepatitis B virus DNA. Methods 1500 patients with hepatitis B from January 2023 to December 2023 were selected for the study data, and all of them were detected by serological markers and real-time fluorescent quantitative PCR. The control standard was the clinical diagnosis results, and the comparison index was the DNA quantitative detection results. Results Among the 1500 confirmed hepatitis B patients, real-time fluorescence quantitative PCR technology detected hepatitis B virus DNA quantification results, which showed that the viral load of 1380 patients (92.00%) could be clearly quantified, while the serological marker method could only clearly quantify the viral load of 1260 patients (84.00%). In addition to technology, factors that may affect the final test results include laboratory operations, sample and nucleic acid extraction, and storage at multiple stages. Conclusion Real time fluorescence quantitative PCR technology has higher sensitivity and accuracy compared to traditional serum blood marker detection methods, and is more suitable for modern laboratory medicine.

For China's booming robot market, robot testing is a key link to ensure the performance and quality of robots, and quality control methods are also crucial for robot testing laboratories. In view of the problems faced by quality control methods in the application of robot testing laboratories, this paper analyzed and discussed the strategies of robot testing laboratories in the application of quality control methods to control testing quality, and effectively improved the accuracy and reliability of testing data and ensured the quality of robot products through the scientific and planned implementation of quality control.