Latest ArticlesIn the process of college education,it is necessary to use various facilities and tools,so many colleges and universities build special laboratories to provide environment and conditions for experimental teaching. Especially in the context of the rapid development of science and technology, schools pay more and more attention to experimental education and improve the quality of laboratory water. They have not only invested a large amount of construction funds, but also formulated more perfect laboratory management plans to promote the continuous improvement of laboratory management. But in the actual management, many colleges and universities still have some problems.Because the scale of laboratory assets has expanded,the number of instruments and equipment has increased, and the value has increased, the management difficulty has also increased, and there are higher requirements for management model innovation. This paper takes university teaching laboratory as the research object, expounds several common teaching laboratory management modes, analyzes the management system and operation mode of China's teaching laboratory, and puts forward the practical strategy of university teaching laboratory management mode.
Food re-inspection refers to food production and operation of food safety supervision and sampling inspection conclusions have objections and in accordance with the law to the market supervision and management department to submit an application for re-inspection, market supervision and management department commissioned the re-inspection of the re-inspection of the backup samples for review and inspection of the behaviour.Re-inspection is an important way for food producers and operators to safeguard their legitimate rights and interests, an important link for market supervision departments to ensure the high quality of food safety supervision work, and an important measure to ensure the relatively fair and stable operation of the market. Because the retest conclusion serves as the final conclusion Therefore, the professionalism of the re-inspection institution is particularly important in the re-inspection work. Based on the perspective of the re-inspection institution, this paper analyzes the risk points of the food re-inspection acceptance work (i. e., contract review), so that the re-inspection institution can avoid potential risks and lay a solid foundation for the smooth progress of the whole re-inspection work.
With the continuous development of China's manufacturing industry and the increase in international trade, the requirements for product quality and safety are constantly increasing. Enterprises need to establish a modern and comprehensive laboratory to provide strong technical support for themselves and ensure the quality and international competitiveness of their products. Taking the laboratory construction of auto parts manufacturing enterprises as an example, this paper studied the process and method of industrial product testing laboratory construction, summarized the general process and method of laboratory construction, Including needs assessment and project planning, site selection and space design, equipment procurement and installation, personnel recruitment and training, quality management system establishment, laboratory safety and environmental protection, laboratory information and digitization, continuous improvement and technological innovation, etc., and put forward relevant suggestions to provide theoretical reference for the construction of related laboratories.
Objective Isolate and identify microorganisms that cause rancidity in vacuum packed rice dumplings, thereby helping enterprises to improve product quality and control of food safety. Methods Bromocresol purple dextrose broth and cooked meat medium base were used to cultivate the acescent rice dumplings; the microorganisms were isolated and purified by scribing in Rose bengal agar, Nutrient agar, the isolated strains were identified by colony morphology observation, cell morphology observation, 16S rRNA sequence analysis, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The isolated strains were inoculated into commercial sterilized rice dumplings respectively to verify that two strains caused rancidity of rice dumplings. Results Two isolated strains, namely Lelliottia amnigena and Wickerhamomyces anomalus, caused rancidity of rice dumplings. Conclusion Lelliottia amnigena and Wickerhamomyces anomalus in vacuum-packed rice dumplings can cause rancidity.
Objective To trace the source of a foodborne disease outbreak caused by consuming smoked meat pancakes contaminated with Salmonella enteritidis. Methods Epidemiological investigations were carried out on 8 patients, samples of relevant environment, food and feces were collected, laboratory tests were carried out according to national standards. After bacteria enhancement, suspicious colonies were selected for biochemical identification, and the six strains of Salmonella detected underwent serum agglutination with standard diagnostic sera, drug sensitivity test and pulsed field gel electrophoresis. Result There were 8 cases of this incident, and out of the 6 samples, 6 strains of Salmonella enteritidis were isolated. The detection rates of Salmonella in the food, environmental samples, and cases feces were all 100%. The drug sensitivity test results showed that all strains were resistant to 6 drugs, and the drug resistance spectrum was CT-CTX - TET - CIP - AMP - AMS. They were sensitive to CHL, SXT, ETP, MEM, CAZ, CZA, TIG, AZM, and AMI. The PFGE bands of the 6 strains were consistent, with the homology of ${100}\%$ , and that showed the same clone. Conclusions This incident is a foodborne disease outbreak caused by consuming smoked meat pancakes contaminated with Salmonella enteritidis.
In order to steadily enhance the laboratory certification and accreditation capabilities, to achieve accurate input, scientific disposal and efficient sharing of complex test data information, to meet the requirements of diversified use. On the basis of mastering the positioning of measurement information management system, this paper focuses on the main contents of laboratory certification and accreditation, improves the system application process, improves the system application path, details the main points of system application, and continuously improves the laboratory operation efficiency.
Objective To develop an ultra high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to identification of the feeding status of turtle and turtle shell components in different batches of Gejie Dingchuan Pills. Methods This study used the identification method of characteristic peptides of Animal medicine for reference, Enzymatic hydrolysis of different batches of Ge jie Ding chuan Pill samples using trypsin, and Identification by using the ultra high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The characteristic ion pairs of Testudinis Carapax et Plastrum and Trionycis Carapax were as detection ion pairs. The positive electrospray ionization and multiple reaction monitoring were adopted. Result: 51 batches of Gejie Ding chuan Pills form 8 companies have all detected turtle shell components, but no other turtle source components have been detected. Conclusion This method is raliable for identification of Testudinis Carapax et Plastrum and Trionycis Carapax form Ge jie Ding chuan Pill.
Objective To analyze the microbiological testing results of hotels, bathing centers, and supermarkets. Methods According to the requirements of the Technical Specification for Public Place Hygiene Monitoring and the Public Place Hygiene Standard Monitoring and Inspection Method, a total of 2372 samples from three types of public places (hotels, bathing centers, supermarkets) were subjected to microbiological testing. Results After analyzing the microbiological testing results of air and public goods from 2021 to 2022, a total of 1124 samples were tested in 2021, of which 1110 were qualified, with a qualification rate of 98.75%; In 2022, a total of 1248 samples were tested, of which 1248 were qualified, with a qualification rate of 100.00%. The microbiological testing results of air and public goods in 2022 were higher than those in 2021 (x2 value=13.586, P<0.05). Conclusion The qualified rate of microbial testing in public places in Jiangyin City in 2022 has increased significantly compared to 2021. However, it is necessary to remain vigilant and strengthen hygiene management to ensure environmental hygiene and personnel health in public places.
Objective To investigate the pesticide residue of fruits and vegetables in Qingpu District of Shanghai, Valuate the risk assessment of pesticide residues in primary and middle school students in Qingpu district. Methods From 2021 to 2022, 252 fruits and vegetables from 7 towns in Qingpu District of Shanghai were collected, and 46 pesticide residues in fruits and vegetables were detected by LC/MS. The acute and chronic dietary exposure risks of primary and secondary school students in Qingpu District of Shanghai was assessed using the results of pesticide residue detection, combined with food consumption in the dietary nutrient intake survey of Qingpu students. Results 15 pesticides of all total 46 pesticides were detected, 12 pesticides exceeded the standard, the highest detection rate was tthiamethoxam (29.76%), the highest exceeding rate was clothianidin (27.78%). Among the 6 kinds of fruits and vegetables, the highest detection rate was strawberry (88.10%), and the highest o exceeding rate was solanaceae (76.19%). The dietary exposure results showed that the acute exposure risks (acute hazardquotient) of methamidophos and dichlorvos weir 562% and 452% respectively, the chronic exposure risk (chronic hazardquotient) of dichlorvos was 434%, and the dietary acute chronic exposure risks of other pesticides were less than 100%. Conclusion There are pesticide residues in fruits and vegetables in Qingpu District of Shanghai. The dietary exposure risk of methamphetamine and dichlorvos in fruits and vegetables is high, which should be paid attention to by the regulatory authorities. The dietary exposure risk of other pesticides is low, but the high detection rate and over-standard rate of fruits and vegetables should be paid attention to.
Objective: This study aims to explore a method for determining the concentration of free amino acids in cell culture media based on ultra performance liquid chromatography (UPLC). Methods Researchers used UPLC technology combined with optimized sample processing Methods to efficiently and accurately determine free amino acids in cell culture medium. Results The research results indicate that the UPLC method can quickly and reliably determine the concentration of free amino acids in cell culture medium, providing an effective means for monitoring and optimizing cell culture in the biopharmaceutical industry. Conclusion Accurate determination of amino acid concentration not only helps to better understand the dynamic changes of key nutrients in cell culture media, but also provides important data support for process optimization in the production process.