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Objective To explore the determination of trace glutathioneby resonance scattering spectral quenching method and improve thesensitivity of the method. Methods In the pH 3.0 phosphoric acid-boricacid-acetic acid buffer solution (BR buffer), $\mathrm{{Fe}}\left(\mathrm{{III}}\right)$ reacts with ${\mathrm{K}}_{4}\left\lbrack {\mathrm{{Fe}}{\left(\mathrm{{CN}}\right)}_{6}}\right\rbrack$ to form Prussian blue ion associates, and the system exhibits a strongresonance scattering effect at ${337}\mathrm{\;{nm}}$ . When glutathione isadded, the sulfhydryl group in glutathione molecules can reduce Fe(III)to Fe(II), causing quenching of the system and weakening the resonancescattering quenching effect. Results The linear regression equation forglutathione within the concentration range of 0.010 to ${2.0\mu }\mathrm{g}/\mathrm{{mL}}$ is ${\Delta l}= {299.77\rho }+{774.52}$ , with an ${r}^{2}$ of 0.9929 and a detection limit of ${0.0024\mu }\mathrm{g}/\mathrm{{mL}}$ . Conclusion This method is simple and highly sensitive, and satisfactoryresults are obtained when used for the determination of glutathione indrugs.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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