Pseudomonas aeruginosa is a conditional pathogenic bacterium that can colonize the upper respiratory tract of pigs. If infected pigs do not have strong immunity or are in a stress state, it is likely to develop Pseudomonas aeruginosa, leading to diseases such as arthritis and meningitis in pigs, seriously damaging the economic benefits of pig farmers. This is very detrimental to the healthy development of China's pig industry. Considering the importance of early diagnosis for the prevention and control of Pseudomonas aeruginosa, this article analyzes Pseudomonas aeruginosa and focuses on the laboratory detection methods of Pseudomonas aeruginosa, in order to lay a foundation for the comprehensive prevention and control of Pseudomonas aeruginosa.

In this paper, the equipment management, personnel training and quality control of the laboratory are comprehensively assessed by adopting quality control methods such as checklist evaluation and traceability management. Taking a food testing laboratory as an example, it quantitatively analyzes its quality management and gives the evaluation and improvement suggestions on equipment management, personnel training and quality control of the laboratory. Meanwhile, in the process of establishing a traceable management system, the management of quality control should also be strengthened to improve the accuracy and reliability of testing. Through the improvement and refinement of the laboratory, it can not only enhance the overall quality level of the testing laboratory, but also contribute to the maintenance of social public safety and health.

Biopharmaceutical cleanroom air conditioning plays an important role in ensuring the cleanliness and stability of a clean environment. By investigating and analyzing the actual situation of clean air conditioning systems in the biopharmaceutical industry, scientific and effective methods for clean air conditioning testing and debugging are proposed, which can accurately evaluate the cleanliness and performance of clean air conditioning systems. Meanwhile, the practical application effect of this method indicates that it can improve the cleanliness and stability of clean environments.

Objective Based on gas chromatography-mass spectrometry (GC-MS) technology and partial least squares analysis (PLS-DA) metabolomics methods, observe the changes in serum metabolites of mice after administration of traditional Chinese medicine Yuanbaocao, and provide a preliminary explanation for the analgesic mechanism. Methods Establish two analgesic models, the hot plate method and the acetic acid writhing method, and use GC/MS to detect and analyze etabolites in mouse serum. After collecting data, PLS-DA method is used to detect and classify endogenous small molecule metabolites in the samples. Results The hot plate analgesic experiment showed that there were extremely significant differences $\left({P<{0.01}}\right)$ in the licking time between the aspirin group, the high and medium dose groups of Yuanbaocao mice, and the model group at 30 and 60 minutes. As the administration time prolonged, Yuanbaocao showed different inhibitory effects on pain symptoms, which was inferred to be related to the high or low blood drug concentration. Compared with the blank group, the number of body twists in the Yuanbaocao group mice decreased significantly $\left({P <{0.01}}\right)$ , indicating that Yuanbaocao has a certain degree of analgesic effect. There are significant differences in the metabolic characteristics of mouse serum, and further screening of metabolic pathways that have a significant impact on data classification identified that taurine, glutathione, arginine and proline, ascorbic acid 5 metabolic pathways are related to pain metabolism. Conclusion The Yuanbaocao group can reverse abnormal changes to a certain extent and has analgesic effects.

In order to solve the problems of significant increase in workload, cumbersome management work, duplication of materials and low review efficiency caused by the increase of open subject application year-by-year, this article elaborates and analyzes the various needs in the open project management process, based on the requirements of the project-oriented management system, and the laboratory open subject management system was constructed based on the requirements of subject management system. The system is developed based on SpringBoot microservice architecture, B/S(Browser/Server) architecture, and Java language development. It can complete project demand reporting, requirements review, requirements selection, project application collection, project application review, project project initiation, mid-term inspection results management, and final inspection, and realize the information management of the whole life cycle of laboratory open projects.

Objective To investigate the levels of veterinary drug residues in chicken and eggs in chicken production bases in Liangshan Prefecture, and evaluate the dietary exposure risk. Methods There were 353 samples of chicken and eggs collected from 17 cities in Liangshan in 2022, then the residues of quinolones and quinoline were detected and analyzed by liquid chromatography-mass spectrometry, and the exposure risk was assessed by point estimate method. Results A total of 221 pieces of chicken were sampled in 2022, among 8 types of veterinary drugs were tested with the detection rate of 25.79%, and the unqualified rate was 1.81%. Among 132 eggs, only enrofloxacin residue was detected, the detection rate was 1.52%, the unqualified rate was 1.52%. The point estimate method was used to assess the dietary risk of veterinary drug residues, and its hazard quotient was lower than 1, indicating that the veterinary drug residues have a lower risk to human health. Conclusion Veterinary drug residues can be detected in some samples of chicken and eggs in Liangshan in 2022, and the risk residues to human health is low, but the health risks of caused by some undetectable veterinary drugs cannot be ignored.

Objective Isolate and identify microorganisms that cause rancidity in vacuum packed rice dumplings, thereby helping enterprises to improve product quality and control of food safety. Methods Bromocresol purple dextrose broth and cooked meat medium base were used to cultivate the acescent rice dumplings; the microorganisms were isolated and purified by scribing in Rose bengal agar, Nutrient agar, the isolated strains were identified by colony morphology observation, cell morphology observation, 16S rRNA sequence analysis, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The isolated strains were inoculated into commercial sterilized rice dumplings respectively to verify that two strains caused rancidity of rice dumplings. Results Two isolated strains, namely Lelliottia amnigena and Wickerhamomyces anomalus, caused rancidity of rice dumplings. Conclusion Lelliottia amnigena and Wickerhamomyces anomalus in vacuum-packed rice dumplings can cause rancidity.

Objective To understand the pollution levels of bromate, coliforms, pseudomonas aeruginosa, nitrite (calculated as ${\mathrm{{NO}}}_{2}$ )] of packaged drinking barreled water in Guangxi in 2022. Methods According to the type of water and test items, all pollutions were tested and judged by GB 8538-2016 “National standard for food safety Test method for drinking natural mineral water”, GB 4789.3-2016 “Food safety national standard food Microbiology test Coliform count”, GB 5009.33-2016 “National standard for food safety Determination of nitrite and nitrate,” GB 19298-2014 “Food safety national standard Packaging drinking water”, GB 2762-2017 “Food safety national standard food Pollutant limits”. Results 204 batches of packaged drinking barreled water were collected. Pseudomonas aeruginosa was deteted in 55 batches with a detection rate of 26.96%. Not a single batch exceeded permitted level of bromate, coliform or nitrite. Conclusion Most products are safe, but the pollution of Pseudomonas aeruginosa is still an outstanding problem. Enterprises should strengthen the cleaning and isinfection of pipes and barrels and standardize transportation storage to ensure product safety.

Objective A method for simultaneous determination of MBAA, DBAA, DCAA, TCAA and ${\mathrm{{ClO}}}_{2}{}^{- }$ in drinking water was established by ion chromatography. Methods The water samples were determined by ${0.22\mu}\mathrm{m}$ qeuosystem filter membrane with a volume of ${500\mu }\mathrm{L}$ . The samples were rinsed with $\mathrm{{KOH}}$ solution gradient and separated by IonPac AS19 anion column. The column temperature was set at ${30}^{\circ }\mathrm{C}$ , and the current of the inhibitor was ${75}\mathrm{\;{mA}}$ . Results The results showed that the linearity ranges of ${\mathrm{{ClO}}}_{2}{}^{- }$ , MBAA, DBAA, DCAA and TCAA were found in the range of ${10}\sim {1000\mu}\mathrm{g}/\mathrm{L},\mathrm{r}> {0.9990}$ , with detection limits $\left({3\mathrm{\;S}/\mathrm{N}}\right)$ of ${0.32},{0.76},{1.30},{0.69},{1.58\mu}\mathrm{g}/\mathrm{L}$ . The standard recoveries were ${80}\%\sim {110}\%$ and RSD (n=6) of the determined values were all less than 5.0%. Conclusion The method was simple, sensitive, can meet the detection requirements of four kinds of Haloaeetic Acids and Chlorite in drinking water. The method can be used for the determination of kour kinds of haloaeetic acids and chlorite in drinking water.

With the trend of globalization and population growth, the physical and chemical testing laboratories of disease control institutions play a crucial role in disease prevention and control. However, these laboratories face a series of safety and quality risks. The purpose of this study is to identify and control safety and quality risks in physical and chemical testing laboratories of disease control institutions. Through literature review and case studies, we analyzed the main risk sources in current laboratory management and proposed a series of measures to address these risks. This study has important guiding significance for improving the safety and quality of physical and chemical testing laboratories in disease control institutions.