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  • Hui-Juan ZHANG
    Laboratory Testing. 2024, 2(8): 82-85.

    Quality control of cement concrete raw materials in highway engineering is a key factor in ensuring engineering quality and durability. This paper analyzes the importance, content and existing problems of cement concrete raw material testing and inspection, and proposes a series of quality control measures. These measures include formulating scientific and reasonable testing and inspection processes, strengthening the management of raw material use and inspection, and optimizing the inspection environment. By taking these measures, the quality of cement concrete engineering can be effectively improved, and the smooth construction and long-term use of highway engineering can be guaranteed.

  • Wei-Wei CAO, Run-Dong QIANG, Chun-Yu ZHANG, Hui-Na BO, Zhi-Ping WANG, Ke-Xin SHI, Ying JIANG
    Laboratory Testing. 2024, 2(8): 25-28.

    Objective To establish a method for determining thecontents of paeoniflorin, cinnamaldehyde and paeonol in Guizhi JiliuPills. Methods The RP-HPLC wavelength switching method was used, theanalysis was achieved on an ES Epic ${\mathrm{C}}_{18}\left({{4.6}\mathrm{\;{mm}}\times {250}\mathrm{\;{mm}},{5\mu}\mathrm{m}}\right)$ column, the mobile phase was acetonitrile-0.1% aqueous solution of phosphoric acid gradient elution, the flowrate was ${1.0}\mathrm{\;{mL}}/\mathrm{{min}}$ , thecolumn temperature was ${25}^{\circ }\mathrm{C}$ and thedetection wavelength were ${230}\mathrm{\;{nm}}$ $\left({0\sim {25}\mathrm{\;{min}}}\right),{290}\mathrm{\;{nm}}\left({{25}\sim {41}\mathrm{\;{min}}}\right)$ and ${274}\mathrm{\;{nm}}\left({{41}\sim {51}\mathrm{\;{min}}}\right)$ . Results The linear ranges of paeoniflorin, cinnamaldehyde and paeonolwere 3.6169~90.4232 μg/mL ( $r={0.9993}$ ),1.8388~45.9695 μg/mL ( $r={0.9999}$ ),2.0240~50.5994 μg/mL ( $r={0.9999}$ ). The average sample recovery rates were95.4%(RSD=1.2%), 93.0%(RSD=0.7%) and 94.2%(RSD=1.2%) respectively. Conclusion This method is accurate, sensitive, and has strong specificity. It can be used for the quality control of Guizhi Jiliu Pills.

  • Ai-Lian HU
    Laboratory Testing. 2024, 2(8): 109-112.

    Objective To discuss the dispersion and correlation of granite in Foshan, analyze the causes of the dispersion and the measures to reduce the dispersion. Methods 10 groups of granite samples from a place in Foshan were selected for laboratory tests of physical and mechanical properties, and the data were analyzed statistically. Results The water content, bulk density, particle density and uniaxial compressive strength of granites in this area have a certain correlation, and there are discretization. Conclusion Analysis of the correlation and discreteness of test results is helpful to improve the reference value of test data and provide theoretical support for engineering applications.

  • Ying-Shuai WU, Sen-Sen LAI, Gong-Hui LI, Long-Hai XIAO, Min-Rong YANG
    Laboratory Testing. 2024, 2(6): 22-27.

    Objective A method was developed for the simultaneous determination of calcium, boron and sodium in Bombyx batryticatus by Inductively coupled plasma mass spectrometry (ICP-MS). Methods Microwave Digestion was used as the pretreatment method. The contents of Ca, B and Na in 25 batches of Bombyx batryticatus were determined by ICP-MS and analyzed by multivariate statistics. Results The three elements had a good linear relationship (r>0.995), the recoveries were in the range of 80%~120%, the reproducibility RSD<5%, the reproducibility RSD<5% and the stability RSD<5% within 1 day. Conclusion the method is simple and accurate, and can be used for the quantitative study of three elements in Bombyx batryticatus. The results can provide reference for the quality control and origin study of Bombyx batryticatus.

  • Yong-Liang PEI, Qin ZHANG
    Laboratory Testing. 2024, 2(6): 69-72.

    The convergence of laboratory testing standardization and automation technologies has had a profound impact on scientific research and industrial applications. Standardization provides a framework for consistency and comparability, while automated systems improve experimental efficiency and reliability of results. This article will explore the importance of standardization of laboratory testing and its integration with automation technology, as well as the importance of compliance with standards for automated systems.

  • Cong MENG
    Laboratory Testing. 2024, 2(6): 54-56.

    With the increasing demand of laboratory teaching and scientific research in colleges and universities, higher requirements are put forward for laboratory safety education, which is also an important content to ensure the safety of teachers and students, great importance must be attached to it. In view of this, colleges and universities should pay attention to laboratory culture, actively establish a safety education system, do a good job of top-level design, establish a sound evaluation mechanism, and gradually optimize educational methods and methods, and then build an integrated safety culture and education system.

  • Lin-Yi CAO, Zhi-Yao HUANG, Ya-Xin HU
    Laboratory Testing. 2024, 2(6): 116-119.

    In order to accurately and efficiently determine the purityof ethyl acetate refined products, the separation was carried outaccording to the programmed heating method based on the analysiscondition of gas chromatograph. The initial temperature was set at ${80}^{\circ }\mathrm{C}$ , and after $4\mathrm{\;{min}}$ , the heating ratestarted at ${20}^{\circ }\mathrm{C}/\mathrm{{min}}$ ,and the termination temperature was ${140}^{\circ }\mathrm{C}$ . The analysisshowed that the separation degree of each peak was greater than 2.0, andthe peak shape was symmetrical, sharp and the baseline was stable.Effective separation is possible. After the quantitative analysis ofarea normalization method, correction factor method and internalstandard curve method, the correction factor method has thecharacteristics of high accuracy, strong stability and economy, and itis more suitable for the determination of ethyl acetate purity than thecorrection factor method.

  • Jin-You DING, Hong YU, Ping-Shuang GONG
    Laboratory Testing. 2024, 2(8): 21-24.

    Objective To establish an extraction process and contentdetermination method for quercetin in Gentiana macrophylla, and optimizethe processing technology of Gentiana macrophylla. Methods This studyused ultrasound assisted extraction to extract quercetin from Gentianamacrophylla, and determined the content of quercetin in four processedGentiana macrophylla decoction pieces by HPLC-ELSD. Results The optimalprocess conditions for ultrasonic extraction of quercetin from Gentianamacrophylla are: ethanol volume fraction of 75%, ultrasonic time of 45minutes, solid-liquid ratio of 1 : 10, and ultrasonic temperature of ${30}^{\circ }\mathrm{C}$ . Thehighest yield of quercetin is ${3.40}\mathrm{{mg}}/\mathrm{g}$ . Conclusion The established extraction process and content determinationmethod for quercetin galls have good repeatability, stability, andreliability, which can provide reference for optimizing the processingtechnology of Gentiana macrophylla decoction pieces.

  • Kui LV
    Laboratory Testing. 2024, 2(8): 91-93.

    Total sulfur in coal is one of the important indicators for evaluating coal quality. In order to effectively and economically utilize coal resources, it is necessary to comprehensively understand the total sulfur content in coal. Based on the principle of coulometric titration and the consideration of man-machine material loop measurement, this paper deeply explores the main factors affecting the measurement accuracy, aiming to significantly improve the accuracy of total sulfur determination through systematic analysis of the influence of various factors on the detection and standardization of the correct use, inspection and maintenance of the instrument.

  • Xiao-Bo SHUAI, Bin CHEN, Wen YU, Ying-Ying HAN, Xiao-Lei JI
    Laboratory Testing. 2024, 2(6): 158-160.

    Objective To explore the pathogenic factors causing outbreaks of bacterial foodborne diseases. Methods Collecting anal swabs, food retention samples, and food processing tool application samples from patients affected by this epidemic, refer to "Microbiological Testing of Food Hygiene" (GB/T 4789.4-2016、GB/T 4789.5-2012、GB/T 4789.6-2016, GB/T 4789.7-2013, GB/T 4789.10-2016) and "National Clinical Testing Operating Procedures" (Fourth Edition). Results Aeromonas sobria was detected in food samples from little yellow croaker and anal swabs from two patients. Aeromonas hydrophila was detected in a kitchen appliance application and one patient's anal swab. Conclusion This was a rare outbreak of foodborne disease caused by mixed Aeromonas, In the diagnosis and treatment of intestinal infectious diarrhea, in addition to considering common intestinal pathogenic bacteria, rare pathogenic bacteria such as Aeromonas should not be ignored to avoid clinical diagnosis and treatment difficulties.