Objective To study the detection method of 11a-progesterone and 11a-progesterone acetate by gel permeation chromatography and liquid chromatography, and to provide technical support for market supervision and enterprise quality control. Methods Samples are extracted by ethyl acetate; use 1:1 ethyl acetate: hexane as eluent; purify by gel permeation chromatography (GPC) method; collect the eluent for ${12}\sim {20}\mathrm{\;{min}}$ ; obtain the purified solution from eluent by rotary evaporation; wait for the determination of liquid chromatography. Use 45:55 acetonitrile: water as mobile phase; use C18 $\left({{250}\times {4.6}\mathrm{\;{mm}}}\right)$ chromatographic column for separation; use photo diode array detector for the determination of 11a-progesterone and 11a-progesterone acetate at ${245}\mathrm{\;{nm}}$ . Results The linear correlation coefficient of this method is above 0.995, the recovery rate is ${90.0}\%\sim {102.6}\%$ and 85.1 $\sim {102.7}\%$ , the relative standard deviation (RSD) is ${0.7}\%\sim {2.5}\%$ and ${0.1}\%\sim {8.7}\%$ , the limit of detection is ${0.3}\mathrm{{mg}}/\mathrm{{kg}}$ and 0.6 $\mathrm{{mg}}/\mathrm{{kg}}$ , and the limit of quantification is ${1.0}\mathrm{{mg}}/\mathrm{{kg}}$ and ${2.0}\mathrm{{mg}}/\mathrm{{kg}}$. Conclusion The method is simple, reliable and accurate, and can be used for the determination of 11a-progesterone acetate and 11a-progesterone acetate in cosmetics.

Objective A method based on performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the determination of salbutamol in aquatic products and their products. Methods The analyte was enzymatic hydrolyzed in ${\mathrm{{NH}}}_{4}\mathrm{{AC}}$ solution overnight at ${37}^{\circ }\mathrm{C}$ , and extracted by ethyl acetate-isopropanol $\left({1 :1,\mathrm{v}/\mathrm{v}}\right)$ . The extract was cleaned up by QuEChERS method. Quantification was achieved by HPLC-MS/MS with multiple reaction monitoring (MRM) using internal standard method. Results The limit of quantification of salbutamol was ${0.5\mu }\mathrm{g}/\mathrm{{kg}}$ . The average recoveries at three spiked levels of 0.5,1.0 and ${5.0\mu }\mathrm{g}/\mathrm{{kg}}$ ranged from 87.8% to 110.7% with the relative standard deviations of ${2.7}\%\sim {9.6}\%\left({\mathrm{n}=6}\right)$ . Conclusion The method has the advantages of simple pretreatment steps and its sensitivity, precision, and recoveries all meet the requirements of residue detection regulations, accurately solving the problem of lack of relevant technical support for monitoring salbutamol residues in aquatic products and their products.

As an advanced intelligent management tool, intelligent scheduling system plays an increasingly important role in laboratory management, which can help laboratory personnel manage laboratory resources more effectively, improve laboratory efficiency, availability and safety, and enhance the laboratory's marketable competitiveness. This paper mainly introducecd the application of the intelligent scheduling system in the laboratory and the problems encountered, put forward the corresponding solutions, and discussed the future development trend of the intelligent scheduling system, which providing a theoretical basis for the subsequent technical development.

"Method" is one of the five elements of total quality management. In the inspection and testing laboratory, "method" refers to the method used in the whole process of testing. Laboratory information management system can combine scientific laboratory management theory and computer technology organically, and provide advanced technical support for the overall improvement of laboratory management level. This paper combined the "method" of the five elements of quality management with the laboratory information management system, conducted in-depth discussions from three aspects: Standard management, electronic original record management and quality system document management, and verified that the laboratory information management system could assist managers to discover and control the key factors affecting the inspection quality, so as to achieve quality improvement and self-perfection of the quality system. Improve the competitiveness of the laboratory.

Ecological environment monitoring refers to the use of physical, chemical, biochemical, ecological and other technological means to monitor and test various elements in the ecological environment, the interrelationships between organisms and the environment, the structure and function of ecosystems. Ecological environment monitoring plays an important role in monitoring environmental pollution, protecting the ecological environment, ensuring public health, and promoting sustainable development. In order to achieve resource sharing and complementary advantages among ecological environment monitoring regions, and improve the accuracy and standardization of environmental monitoring data, this paper explored the Methods, technologies, and implementation paths of regional integration of information systems in the field of environmental monitoring from the perspective of ecological environment monitoring quality management. Based on practical cases, this paper elaborated on the implementation path and Methods, including how the contracting and construction units participate and cooperate, how to implement in stages, etc, provided a detailed introduction to the design concept, functions, and interrelationships of each module of the integrated system, and summarized the effectiveness and significance of regional integration in the inspection and testing industry which provides theoretical support for the reform and innovation in the field of ecological environment monitoring.

Objective The accuracy of the test results was evaluated by the interlaboratory comparison of the blind samples, and the test quality and test ability were improved. Methods According to the “National Standard for Food microbiology inspection of food safety” and other Methods for the 2012-2023 assessment of blind samples for testing. Results During the 2012-2023 Blind examination in Yizheng’s CDC Microbiology Laboratory, 3 strains of Enterocoliform Yersinia, 5 strains of Bacillus cereus, 4 strains of Klebsiella (E. sakazakii) and 2 strains of Enterobacter cloacae were detected, 3 strains of Enterobacter aerogenes, 3 strains of Streptococcus hemolyticus, 1 strain of Streptococcus $A,2$ strains of Streptococcus $B,3$ strains of salmonella,1 strain of salmonella enteritidis, 2 strains of Salmonella enterica, two strains of Liszt. Escherichia coli, Shigella, Citrobacter freundii, Pseudomonas aeruginosa, Vibrio alginolyticus, Vibrio vulnificus, Vibrio parahaemolyticus, Staphylococcus epidermidis. Conclusion From 2012 to 2023, all the results were correct, and the combination of manual and instrument can improve the detection capability and quality of the laboratory.

Objective To understand the pollution levels of bromate, coliforms, pseudomonas aeruginosa, nitrite (calculated as ${\mathrm{{NO}}}_{2}$ )] of packaged drinking barreled water in Guangxi in 2022. Methods According to the type of water and test items, all pollutions were tested and judged by GB 8538-2016 “National standard for food safety Test method for drinking natural mineral water”, GB 4789.3-2016 “Food safety national standard food Microbiology test Coliform count”, GB 5009.33-2016 “National standard for food safety Determination of nitrite and nitrate,” GB 19298-2014 “Food safety national standard Packaging drinking water”, GB 2762-2017 “Food safety national standard food Pollutant limits”. Results 204 batches of packaged drinking barreled water were collected. Pseudomonas aeruginosa was deteted in 55 batches with a detection rate of 26.96%. Not a single batch exceeded permitted level of bromate, coliform or nitrite. Conclusion Most products are safe, but the pollution of Pseudomonas aeruginosa is still an outstanding problem. Enterprises should strengthen the cleaning and isinfection of pipes and barrels and standardize transportation storage to ensure product safety.

Objective Isolate and identify microorganisms that cause rancidity in vacuum packed rice dumplings, thereby helping enterprises to improve product quality and control of food safety. Methods Bromocresol purple dextrose broth and cooked meat medium base were used to cultivate the acescent rice dumplings; the microorganisms were isolated and purified by scribing in Rose bengal agar, Nutrient agar, the isolated strains were identified by colony morphology observation, cell morphology observation, 16S rRNA sequence analysis, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The isolated strains were inoculated into commercial sterilized rice dumplings respectively to verify that two strains caused rancidity of rice dumplings. Results Two isolated strains, namely Lelliottia amnigena and Wickerhamomyces anomalus, caused rancidity of rice dumplings. Conclusion Lelliottia amnigena and Wickerhamomyces anomalus in vacuum-packed rice dumplings can cause rancidity.

With the trend of globalization and population growth, the physical and chemical testing laboratories of disease control institutions play a crucial role in disease prevention and control. However, these laboratories face a series of safety and quality risks. The purpose of this study is to identify and control safety and quality risks in physical and chemical testing laboratories of disease control institutions. Through literature review and case studies, we analyzed the main risk sources in current laboratory management and proposed a series of measures to address these risks. This study has important guiding significance for improving the safety and quality of physical and chemical testing laboratories in disease control institutions.

Objective To analyze and identify a strain numbered sznj2009. Methods A strain of sznj2009 was isolated from aquatic products, and the colony morphology, biochemical characteristics and biological characteristics of the strain were analyzed and identified. Results The strain showed round, smooth, small colorless colonies on the Vibrio chromogenic plate; Round, flat yellow colonies with opaque centers but transparent edges on CC and mCPC plates; Round, milky white colony on 3% sodium chloride tryptone soybean agar plate. By 16s rDNA sequence analysis found that the strain and vibrio harveyi (ATCC33843) closer, high homology, the evolution of the similarity of 100%. Conclusion The strain sznj2009 isolated from aquatic products was Vibrio harveyi. In the traditional biochemical identification and detection of Vibrio vulnificus, through comparison, it is found that Vibrio harveyi is similar to Vibrio vulnificus in colony characteristics and biochemical characteristics, however, the two strains can be clearly distinguished in the plate and biochemical reaction of Vibrio vulnificus, which can improve the identification accuracy of Vibrio vulnificus and provide reference for the traditional isolation and identification of Vibrio harveyi.