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Objective To establish an extraction process and contentdetermination method for quercetin in Gentiana macrophylla, and optimizethe processing technology of Gentiana macrophylla. Methods This studyused ultrasound assisted extraction to extract quercetin from Gentianamacrophylla, and determined the content of quercetin in four processedGentiana macrophylla decoction pieces by HPLC-ELSD. Results The optimalprocess conditions for ultrasonic extraction of quercetin from Gentianamacrophylla are: ethanol volume fraction of 75%, ultrasonic time of 45minutes, solid-liquid ratio of 1 : 10, and ultrasonic temperature of ${30}^{\circ }\mathrm{C}$ . Thehighest yield of quercetin is ${3.40}\mathrm{{mg}}/\mathrm{g}$ . Conclusion The established extraction process and content determinationmethod for quercetin galls have good repeatability, stability, andreliability, which can provide reference for optimizing the processingtechnology of Gentiana macrophylla decoction pieces.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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