Water is a necessary substance for all living things on the earth, so it is very important to monitor pollutants in the water environment. At present, many analytical methods have been proposed for the detection of water pollutants. Portable sensor detector is of special importance because of its simple operation, low cost and portability. This paper first introduces the application of portable rapid detection technology, and further reviews the research progress of rapid detection technology for biological and non-biological pollutants in water bodies. Finally, the current research progress is summarized, which provides a reference for the further development of rapid detection technology.

With the continuous development of coal mining, the safe operation of coal mine mechanical and electrical equipment is very important. This paper discusses the application of ultrasonic detection technology in the safety detection of coal mine mechanical and electrical equipment, expounds the principle and characteristics of ultrasonic detection technology, and analyzes its specific application methods in the detection of various faults and defects of coal mine mechanical and electrical equipment, including the detection of mechanical parts and electrical equipment. By discussing the measures of improving the application effect of ultrasonic detection technology, the effectiveness of ultrasonic detection technology in the safety detection of coal mine electromechanical equipment is shown for its reference.

Objective Compare the performance differences of threeprotein content determination kits (Lowry method of Aimei, BCA method ofKangwei, and BCA method of Thermo Fisher) in detecting micro $\left({0 \sim {20\mu}\mathrm{g}/\mathrm{{mL}}}\right)$ protein. Select the mostaccurate and reliable reagent kit for the detection of trace proteinsamples. Methods Use these three test kits to detect micro proteincontent, compare their accuracy, sensitivity, linearity andrepeatability, and evaluate their equivalence. Results The recoveryrates of the three test kits for detecting trace amounts $\left({{1.25}\sim {20\mu}\mathrm{g}/\mathrm{{mL}}}\right)$ of protein were all withinthe range of 75%~120%, demonstrating qualified accuracy; Thermo FisherBCA meets the acceptable precision range (RSD $\leq 6\%$ ) in the range of ${2.5\mu }\mathrm{g}/\mathrm{{mL}}$ to ${20\mu }\mathrm{g}/\mathrm{{mL}}$ ,while Aimei Lowry and Kangwei BCA meet the acceptable precision range(RSD $\leq 6\%$ ) in the range of ${5\mu }\mathrm{g}/\mathrm{{mL}}$ to ${20\mu }\mathrm{g}/\mathrm{{mL}}$ ,demonstrating good repeatability; the correlation coefficient ${\mathrm{R}}^{2}$ of the standard curvesof the three test kits $\left({0\sim {20}\mathrm{{\mu g}}/\mathrm{{mL}}}\right)$ are all higherthan 0.980 . The linearity of Aimei Lowry is slightly poor, with ${\mathrm{R}}^{2}$ of 0.981 . The linearityof Kangwei BCA is qualified, with ${\mathrm{R}}^{2}$ of 0.991 . The linearityof Thermo Fisher BCA is the best, with ${\mathrm{R}}^{2}$ of 0.993; The abovemethodological validation results indicate that the minimumquantification limits for the sensitivity of the three test kits are ${5\mu }\mathrm{g}/\mathrm{{mL}}$ forthe Aimei Lowry method, ${5\mu}\mathrm{g}/\mathrm{{mL}}$ for the Kangwei BCA method, and ${2.5\mu }\mathrm{g}/\mathrm{{mL}}$ for theThermo Fisher BCA method, respectively; Paired curves of values detectedby three reagent kits for the same series of BSA concentrations andtheir correlation coefficients were all higher than 0.990 . ConclusionThrough experiments, we found that each reagent kit exhibited differentperformance in different concentrations of BSA samples, among which theThermo Fisher BCA method performed the best in terms of accuracy andrepeatability, and there was a certain correlation between these threemethods.

Phosphorus is one of the important nutrient elements in the soil, which has an important influence on crop growth. The availability and availability of phosphorus elements vary in their morphology. Accurate and rapid analysis of phosphorus morphology in soil is crucial for rational fertilization and increasing crop yield. This paper discusses the rapid analysis method of phosphorus elements in soil morphology, introduces the determination of phosphorus including instrument working conditions setting, working curve and ICP method. The application of ICP method as a simple and easy detection method in phosphorus analysis was discussed, through the actual detection method to verify the accuracy and reliability of different detection methods. Finally, the comprehensive application of various methods can quickly and accurately analyze the morphology of phosphorus in soil.

Objective To establish a spectrophotometric evaluationmethod for determining sulfur dioxide uncertainty in formaldehydeabsorption fluid. Methods According to JJF 1059-2012, Evaluation andrepresentation of measurement Uncertainty, establish a mathematicalmodel to calculate each uncertainty component and synthetic uncertainty. Results The uncertainty introduced by repeated measurement is 0.27%; thestandard curve preparation is 0.40%; the standard curve drawing andfitting is 0.20%; the instrument measurement is 0.35%; the syntheticrelative standard uncertainty is 0.63%, and the extended uncertainty is ${0.008}\mathrm{{mg}}/\mathrm{L}$ . Conclusion This method is suitable for measuring the uncertaintyassessment of sulfur dioxide in formaldehyde absorption fluid.

Objective To clarify the detection status and antimicrobial sensitivity of clinically isolated Pseudomonas aeruginosa, assist clinical doctors in using antibiotics based on evidence, delay the rate of bacterial resistance, and intervene purposefully in hospital acquired infections. Methods Pseudomonas aeruginosa cultured, isolated, and identified from clinical samples submitted by our company from January 1, 2023 to December 31, 2023 were collected. According to the latest standard CLSI-M100-ED33, the bacteria were identified using a time-of-flight mass spectrometer, and a fully automated identification and drug susceptibility analysis system was used for drug susceptibility testing. Results Pseudomonas aeruginosa was mainly isolated from elderly men over 60 years old. Sputum was the main source of infection specimens of Pseudomonas aeruginosa. The infected patients were mainly distributed in respiratory and internal medicine departments. The isolated Pseudomonas aeruginosa had high resistance to antibiotics such as amtraxam and ciprofloxacin, and high sensitivity to amikacin and tobramycin, and the overall antibiotic resistance showed a downward trend. Conclusion Strict detection of bacterial resistance and drug resistance genes should be carried out in clinical medicine, so that antibiotics can be used more rationally.

In the context of increasingly serious global diseases and health problems, the importance of biosafety concept has become increasingly prominent. As a country with biological diversity, China faces more serious public health risks. Laboratory safety, as an important link to strengthen biosafety protection, especially for the safety of pathogenic microorganism laboratories, is an important link to reduce the exposure and spread of dangerous goods, and ensure public health and life safety. Scientific laboratory safety management is of great importance. In order to improve the management level of pathogenic microbiology laboratory, the application of digital technology and the construction of information laboratory record management platform are of great application significance, which can effectively realize an efficient, integrated and standardized laboratory management mechanism, and provide support for the optimization and construction of public facilities such as pathogenic microbiology laboratory. This study will mainly explore the optimization path of the construction of the pathogenic microbiology laboratory record management platform, combined with the development requirements of the industry, social needs, etc., to build an efficient management system and improve the quality of laboratory safety management.

University laboratory is an important place for scientific research, and the safety management of laboratory is directly related to the life safety of teachers and students, and will affect the smooth progress of scientific research. However, the traditional management mode has a certain lag, especially in the current situation of more and more types of various tests and more complex, it is very important to actively adopt efficient management to strengthen the management of university laboratory. Capability maturity model is an advanced management concept, which has been gradually introduced into the management of many organizational structures in recent years, and gradually introduced into the management of university laboratories. Therefore, this paper makes an in-depth analysis of university laboratory security management under the guidance of capability maturity model.

Objective A 4-mercaptobenzoic acid/polypeptide-modifiedgold nanoparticle modified surface enhanced Raman spectroscopy (SERS)sensor was developed for rapid detection ofAg ${}^{+ }$ in water. Methods $\;\mathrm{A}{\mathrm{{Ag}}}^{+ }$ surfaceenhanced Raman spectroscopy sensor was constructed by combining aspecificAg ${}^{+ }$ peptiderecognition sequence (RFPRGGDD) with 4-mercaptobenzoic acid, and datawas read using a portable Raman spectrometer. Results In theconcentration range of ${10}\sim {850}\mathrm{{nmol}}/\mathrm{L}$ ,the linear relationship was good, the correlation coefficient $\left({r}^{2}\right)$ was 0.98345, thelimit of detection was ${36}\mathrm{{pmol}}/\mathrm{L}$ , thestandard recoveries were ${95.28}\%\sim {102.9}\%$ , and therelative standard deviations were 0.79%~3.73%. Conclusion The method has high response speed, good selectivity and low detection limit, and is suitable for rapiddetection of Ag+ in water.

In order to build a comprehensive and in-depth university laboratory safety management system and systematically eliminate laboratory safety hazards, this article takes 259 core literature on university laboratory safety management systems collected from "China National Knowledge Infrastructure" from 2008 to 2024 as the basic data, and uses Cite Space software to visualize the research authors, research institutions, keywords, and other aspects, further analyzing the research status and development trends. The results indicate that the number of publications in this field has been increasing year by year, but there is relatively little interaction among researchers, and research institutions are relatively independent with unclear collaborative relationships. Research hotspots mainly focus on safety awareness, system construction, risk assessment, safety culture, emergency management, security system, and other aspects. In the future, emphasis should be placed on building laboratory safety management systems from these hotspots.