Objective This study aims to improve the accuracy and reliability of the determination of active ingredient content in Enteritis Ning Granules by optimizing high-performance liquid chromatography (HPLC) technology, and comprehensively evaluate the uncertainty of the determination results. Methods In this study, the Agilent 1260 HPLC system was used to determine the content of gallic acid active ingredient in Enteritis Ning Granules by optimizing chromatographic conditions such as mobile phase ratio, detection wavelength, column temperature, and solvent. At the same time, establish mathematical models toquantify multiple sources of uncertainty such as instrument errors, operational errors, and sample preparation errors. Results The optimized chromatographic technique significantly improved the sensitivity and accuracy of detecting active ingredients in Enteritis Ning Granules. The content of gallic acid is${27.5}\mathrm{{mg}}$, the chromatographic peak shape is symmetrical, and the separation degree and theoretical plate number have reached the ideallevel. The uncertainty analysis shows that the total uncertainty is small$\left({{U}_{\text{total }}={5.40}\%}\right)$, indicating that the optimized chromatographic technique has high reliability. Conclusion This study successfully optimized the HPLC method for determining the content of Enteritis Ning Granules, and demonstrated the accuracy and reliability of the method through a comprehensive evaluation of uncertainty. This provides a more accurate and reliable analytical method for the quality control of Enteritis Ning Granules, and also offers a new methodology for the modernization analysis of traditional Chinese medicine preparations.

Geotechnical compaction test is an important means to determine the relationship between the optimal dry density and the optimal water content of soil under a certain compaction work, which can be used to understand the compaction performance of soil, and has important significance to ensure the quality of engineering foundation construction. On the basis of comprehensive analysis of influencing factors of geotechnical compaction test, combined with the author's engineering practice experience, this paper puts forward strategies to strengthen the control of the authenticity and effectiveness of geotechnical compaction test results, aiming at improving the quality and efficiency of geotechnical tests and ensuring the reliability and functionality of geotechnical tests.

With the needs of national scientific research and teaching in the new era, the types of chemicals in university laboratories are gradually increasing, and the treatment of laboratory waste liquid has attracted more and more people's attention. Improper treatment methods will bring harm to the surrounding environment and even people's healthy life. Article combined with the existing laboratory waste liquid source management is not standard, waste liquid disposal channel is not open, compare three common laboratory waste liquid disposal way, draw lessons from the advanced experience of foreign university waste liquid disposal, it is pointed out that it is necessary to clarify the main responsibility, strengthen source management, strengthen specification consciousness, through process management, multilateral coordination, dredge waste disposal way to achieve laboratory waste liquid feasible, easy, legal, compliance emissions.

Objective Through the statistical analysis of the actual detection results of Down's disease screening and the pregnancy outcome of follow-up in Zhuhai, the health economics evaluation was carried out. Methods The data of 31967 pregnant women who underwent serum screening (SS) and non-invasive prenatal testing (NIPT) during the same pregnancy in Zhuhai City from 2018 to 2021 were retrospectively analyzed. The cost-benefit analysis was conducted according to 5 different screening strategies. Results Among 31,967 pregnancies, 38 were Down syndrome babies (including 3 pregnant women aged over 35 years), with a Down syndrome incidence of 1/841. Serological screening detected 1,649 cases of high risk of T21, of which 21 were true positives, with a positive predictive value of 1.27% and a detection rate of 55.3%; NIPT screening detected 49 cases of high risk of T21, 38 cases of Down syndrome babies, with a positive predictive value of 77.55% and a detection rate of 100%. The total cost generated by calculation strategy 4 was the lowest, about 27.44 million yuan. Conclusion NIPT can be used as primary screening test instead of serological screening method in prenatal Down's screening for secondary prevention and control of birth defects, which has great social and economic benefits.

Objective To evaluate the performance of HORIBA ABX Yumizen H550 automatic blood cell analyzer. Methods According to the national health industry standard Quality Requirements for Analysis of Routine Items in Clinical Hematology Examination (WS/T 406-2012), the automatic blood cell analyzer was evaluated for background counting, carrying contamination, in-batch precision, linearity, accuracy, comparability of results in different suction modes, and comparability of results in the laboratory (the reference instrument was Sysmex XS800i automatic blood cell analyzer). Results The detection values of background count of all items of the automatic blood cell analyzer were met the requirements of industry standards and manufacturer’s instrument manuals, and the carryovers of white blood cell (WBC) count, red blood cell (RBC) count, hemoglobin (HGB) and blood platelet (PLT) count of that were all passed. The results of within-run precision and inter-day precision were less than the requirements of the specifications of instrument and were far less than the requirements of standard WS/T 406-2012. Within the linearity range, the slopes of the linear regression equation were within the range of$1 \pm {0.05}$. The correlations coefficient(r)of these equations were larger than 0.975 . The pass rate that the results compared with that of Sysmex XS800i were larger than 80%. The compared results of intra clinical laboratory indicated the trueness of instrument, the outcome bias between different suction patterns. Conclusion Yumizen H550 automatic blood cell analyzer has favorable performance, accurate and reliable results. It’s an ideal automatic blood cell analyzer.

Objective To explore the application effect of C-reactive protein combined with blood routine and blood culture in the diagnosis of bacteremia. Methods In this study, 69 patients with suspected bacteremia examined from May 2023 to May 2024 were selected as the observation group. In order to more intuitively understand the application value of the three combined detection methods, 69 healthy patients in the same period were selected as the experimental group. Analysis of blood routine indexes, strain distribution in blood culture and C-reactive protein detection data in both participating groups. Results In the blood cultures. Results A total of 8 strains were detected in the blood culture of 69 suspected bacteremia patients in the observation group. Among the two strains with high detection rate, except gram-negative bacteria and gram-positive bacteria, the two strains with the highest detection rate were staphylococcus 25 strains. Escherichia coli 16 granules, and 16 E. coli with detection rate of${23.19}\%$. WBC$\left({{15.45}\pm {2.18}}\right)\times {10}^{9}/\mathrm{L}$, NEU$\left({{81.24}\pm {2.84}}\right)\%$, LYM$\left({{53.24}\pm {2.68}}\right)\%$, C-reactive protein levels (53.24±2.68) mg/L, WBC (6.45±1.28)×10${}^{9}$/L, NEU (62.41±2.34)%, LYM (26.45±2.18)%, and C-reactive protein levels$\left({{6.28}\pm {1.25}}\right)\mathrm{{mg}}/\mathrm{L}$. WBC, NEU, LYM and C-reactive protein levels in patients with suspected bacteremia in the observation group were significantly higher than those of healthy patients in the experimental group$\left({P <{0.05}}\right)$. Of the 69 suspected bacteremia, 66 were positive and 3 were negative. Among them, 63 cases were positive in blood culture, 58 cases were positive in blood routine test, 56 cases were positive in C-reactive protein test, and 66 cases were positive in C-reactive protein combined with blood routine and blood culture test. Among the four detection methods, C-reactive protein combined with blood routine and blood culture test were no different with the confirmed results. Conclusion In clinical diagnosis and detection of suspected bacteremia patients, C-reactive protein combined with blood routine and blood culture can maximize the accuracy of detection data, and the combined diagnosis of the three is of higher clinical value.

Testing laboratory resources are an important support for teaching and scientific research in universities. How to evaluate the utilization efficiency of testing laboratories through big data analysis and make targeted optimization suggestions is of great significance to improving the testing capabilities and resource utilization efficiency of universities. Based on the big data analysis method, this article starts from the aspects of testing laboratory usage data collection, storage, analysis, visualization, etc., builds a testing laboratory usage efficiency evaluation model, and proposes an optimization strategy. The study found that the evaluation of testing laboratory usage efficiency needs to comprehensively consider multi-dimensional indicators such as frequency of use, duration, equipment utilization, sample processing capacity, testing accuracy, testing cycle, etc. Data collection and processing are key links. In response to the problems discovered in the assessment, optimization measures such as strengthening planning management, improving the reservation system, optimizing layout, and establishing incentive mechanisms were proposed. This study has certain reference value for promoting the informatization of testing laboratory management and improving testing capabilities and efficiency.

In order to achieve real-time monitoring of the status of fire-fighting equipment, improve detection efficiency and real-time data, this paper mainly studies the remote detection of fire-fighting products using advanced network data collection and security technologies. The remote detection system for fire protection products faces security risks in data transmission and storage, including data theft, tampering, and network attacks. It must apply encryption technology, identity authentication, multi factor authentication, firewalls, and intrusion detection systems. In addition, it is necessary to comply with data privacy regulations and perform data anonymization and de identification processing to protect user privacy, which is of great significance for achieving remote detection of fire protection products.

Objective To study the application of SERS technique in the quantitative detection of antibiotic residues in poultry meat and evaluate its potential in food safety monitoring. Methods The SERS principle and enhancement factor calculation were introduced, and the influence of the shape and size of nanoparticles on SERS effect was analyzed experimentally and simulated. Different concentrations of antibiotic residues were detected through SERS, and the detection sensitivity and linear response were evaluated. Results SERS was reliable at${0.8}\mathrm{{nmol}}/\mathrm{L}$antibiotic concentration, the linear response range was${0.1}\sim {1000}\mathrm{{nmol}}/\mathrm{L}$, the${r}^{2}$was 0.999, and the enhancement factor was stable at${10}^{6}$. Conclusion SERS technology has high sensitivity and good linear response, which is suitable for the detection of antibiotic residues in food safety monitoring.

Objective To establish a titration method for evaluating the uncertainty in the determination of thiomersal content in adsorbed diphtheria, tetanus, and acellular pertussis combined vaccine, and to identify the sources of significant uncertainty, improve laboratory detection ability. Methods The titration method was established according to the first method of the General Rules 3115 in the 2020 edition of the Pharmacopoeia of the People’s Republic of China, a mathematical model was established, and the sources of uncertainty in the detection process were analyzed to synthesize the standard uncertainty and extended uncertainty. Results In the determination of thiomersal content, all influencing factors have a certain effect. After type A evaluation and type B evaluation, the extended uncertainty${U}_{\mathrm{{rel}}}= {4.72}\%\left({K = 2}\right)$was obtained. Conclusion After evaluating the uncertainty introduced by each factor, it was found that the factors introducing significant uncertainty are mainly the titration process of the test sample. It is suggested that the titration process in low-temperature and other conditions be considered in subsequent method improvements.