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Objective Establish a ultra high performance liquidchromatography (UPLC) method for the simultaneous determination of sevenginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd in ginseng medicinalmaterials. Methods Using CAPCELL PAK ${\mathrm{C}}_{18}\mathrm{{MG}}\left({{2.0}\mathrm{\;{mm}}\times {150}\mathrm{\;{mm}},{2.0\mu}\mathrm{m}}\right)$ Chromatographic column with gradientelution of acetonitrile-0.05% phosphoric acid solution as mobile phase,flow rate of ${0.38}\mathrm{\;{mL}}\cdot {\mathrm{{min}}}^{-1}$ ,detection wavelength of ${203}\mathrm{\;{nm}}$ , column temperatureof ${30}^{\circ }\mathrm{C}$ , andinjection volume of ${1\mu}\mathrm{L}$ . Results All 7 ginsenosides can be completelyseparated and have a good linear relationship $\left({{r}^{2}> {0.999}}\right)$ withina certain linear concentration range, with an average recovery rate of ${97.22}\%\sim {104.56}\%$ ; Therelative standard deviation (RSD) is ${0.4}\%\sim {1.8}\%\left({n = 9}\right)$ ,and the precision RSD is ${0.57}\%\sim {1.42}\%$ . Conclusion Thismethod has strong specificity, excellent precision, good accuracy, andmore efficient detection process, and can be used for the determinationof seven ginsenosides in ginseng medicinal materials.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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