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Extraction of Cerasus humilis Polyphenol by the Probe-type Ultrasound Assisted with NADES and Its Enrichment Mechanism and Antioxidant Activity
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Shutong CHEN1, 2, Jin LIU2, Yaru LI2, Ailin ZHANG1, *, Wenhai KUANG2, Yujie XIONG2, Shu HAO2, Si CHEN2, Huahao ZHANG2, Shenhua JIANG1, 2, *
Science and Technology of Food Industry | 2026, 47(4) : 313 - 323
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Science and Technology of Food Industry | 2026, 47(4): 313-323
• Processing Technology •
Extraction of Cerasus humilis Polyphenol by the Probe-type Ultrasound Assisted with NADES and Its Enrichment Mechanism and Antioxidant Activity
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Shutong CHEN1, 2, Jin LIU2, Yaru LI2, Ailin ZHANG1, *, Wenhai KUANG2, Yujie XIONG2, Shu HAO2, Si CHEN2, Huahao ZHANG2, Shenhua JIANG1, 2, *
Affiliations
  • 1School of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China
  • 2Jiujiang Key Laboratory of Food Processing and Safety, Jiujiang Key Laboratory of Conservation and Development of Major Cyprinidae Fish, School of Pharmacy and Life Science, Jiujiang University, Jiujiang 332000, China
Published: 2026-02-15 doi: 10.13386/j.issn1002-0306.2025030264
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In this study, probe-type ultrasound assisted natural deep eutectic solvents (NADES) were used for the extraction of Cerasus humilis polyphenols (CHP). The extraction parameters were optimised by single factor and orthogonal experiments, and the optimal resin was selected based on adsorption/desorption capacity. The adsorption mechanism of the resin on CHP was investigated by resin adsorption kinetics and isothermal equilibrium experiments, and the composition and antioxidant activity of CHP were analysed. The results showed that choline chloride and citric acid were the optimal solvents for extracting CHP with a solid-liquid ratio of 1:50 (g/mL), ultrasonic power of 420 W, NADES moisture content of 35% (V/V) and ultrasonic extraction for 30 min. The CHP extraction content reached 491.25 mg/100 g dry weight. AB-8 had favorable adsorption/desorption capacity and desorption rate (80.20%, 57.81% and 46.36%, respectively) and was selected as the optimal resin. The adsorption process was monolayer adsorption, and low temperature was conducive to adsorption. Seventeen polyphenolic compounds, including chlorogenic acid, catechins and epicatechins, were identified from the purified extract by LC-MS2. In addition, CHP exhibited strong antioxidant activity with SC50 values of 0.059 and 0.032 mg/mL for DPPH and ABTS+ radical scavenging, respectively. This study may provide a theoretical basis for the green extraction and application of functional agents in Cerasus humilis.

Cerasus humilis  /  polyphenol  /  natural deep eutectic solvents (NADES)  /  probe-type ultrasound-assisted extraction  /  macroporous resin adsorption mechanism  /  antioxidant activity
陈姝彤, 刘谨, 李雅茹, 张爱琳, 况文海, 熊钰洁, 郝澍, 陈思, 张化浩, 江慎华. 脉冲超声辅助天然低共熔溶剂提取欧李多酚及其富集机制和抗氧化活性的研究. 食品工业科技, 2026 , 47 (4) : 313 -323 . DOI: 10.13386/j.issn1002-0306.2025030264
Shutong CHEN, Jin LIU, Yaru LI, Ailin ZHANG, Wenhai KUANG, Yujie XIONG, Shu HAO, Si CHEN, Huahao ZHANG, Shenhua JIANG. Extraction of Cerasus humilis Polyphenol by the Probe-type Ultrasound Assisted with NADES and Its Enrichment Mechanism and Antioxidant Activity[J]. Science and Technology of Food Industry, 2026 , 47 (4) : 313 -323 . DOI: 10.13386/j.issn1002-0306.2025030264
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Year 2026 volume 47 Issue 4
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doi: 10.13386/j.issn1002-0306.2025030264
  • Receive Date：2025-03-19
  • Online Date：2026-10-24
  • Published：2026-02-15
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  • Received：2025-03-19
Affiliations
    1School of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China
    2Jiujiang Key Laboratory of Food Processing and Safety, Jiujiang Key Laboratory of Conservation and Development of Major Cyprinidae Fish, School of Pharmacy and Life Science, Jiujiang University, Jiujiang 332000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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