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Outline
Objective: This study aimed to investigete the potential and molecular mechanisms of Oudemansiella raphanipies extract (ORE) in ameliorating lipid metabolism dysregulation. Methods: A lipid accumulation model was established in HepG2 cells using free fatty acids (FFA) to investigate the effects of ORE on obesity-associated hepatic steatosis. Oil red O staining were used to detect lipid deposition within cells. Relevant cellular parameters were measured using biochemical assay kits. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were employed to elucidate the molecular mechanisms underlying ORE's improvement of lipid metabolism. Results: ORE effectively reduced lipid accumulation in HepG2 cells. It significantly downregulated the expression of adipogenesis-related genes and proteins, including C/EBPα, PPARγ, SREBP-1c, and FAS. Additionally, ORE significantly reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels while enhanced the activity of superoxide dismutase (SOD) and catalase (CAT). Conclusion: ORE can improve the lipid metabolism level of lipid-deposited HepG2 cells. The underlying mechanism may be achieved by downregulating C/EBPα and PPAR-γ to further inhibit the SREBP-1c/FAS synthesis pathway. Concurrently, ORE alleviates oxidative stress in HepG2 cells, potentially through reducing ROS, MDA, enhancing SOD and CAT activities, thereby modulating the cellular redox system.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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