Full
Outline
To investigate the extraction process of Ganoderma lucidum total phenols (GLTP), response surface methodology was employed to optimize ultrasound-assisted extraction parameters. The phenolic composition of GLTP was analyzed using ultra performance liquid chromatograph mass spectrometer (UPLC-QTOF-MS/MS), and its in vitro antioxidant activity along with protective effects against oxidative damage in neural cells were evaluated. The results revealed that the optimal extraction process, comprising an ultrasonic power of 280 W, ethanol concentration of 78%, liquid-solid ratio of 60:1 (mL/g), and ultrasonic time for 62 min, resulted in a GLTP content of 4.52±0.09 mg/g. Eight phenolic substances were detected in the GLTP, seven of which were identified in G. lucidum extracts for the first time. The half-maximal inhibitory concentrations of GLTP for scavenging 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt anion radicals (ABTS+), and hydroxyl radicals (·OH) were 0.6536, 1.087, and 0.3734 mg/mL, respectively, and the ferric reducing antioxidant power (FRAP) assay value was 9.04±0.25 mmol/L. Cellular experiments revealed that, compared with an model group, when treated with 100 μg/mL GLTP, cell survival increased from 50.73% to 81.11%, lactate dehydrogenase (LDH) release decreased from 224.48% to 158.10%, the contents of reactive oxygen species (ROS) decreased from 158.79% to 103.63%, and the concentration of Ca2+ was reduced from 157.94% to 118.62%. Furthermore, this study detected a reduction in malondialdehyde (MDA) content from 10.28 to 6.91 nmol/mg protein, whereas the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) increased from 2.67, 1.68, and 536.12 U/mg protein to 6.59, 3.20, and 757.03 U/mg protein, respectively. These results indicated that GLTP significantly mitigated H2O2-induced oxidative damage in neuronal cells. In conclusion, under these optimized conditions, G. lucidum total phenol extract demonstrates excellent antioxidant and neuroprotective effects, providing a theoretical foundation for the development of G. lucidum resources and their potential applications in functional foods.
101
45
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
