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Objective: In this study, we investigated the therapeutic effects and underlying mechanisms of Laetiporus sulphureus in a mouse model of food allergy (FA). Methods: Mice were randomly divided into the following four groups: control, FA, L. sulphureus prevention and L. sulphureus treatment. The FA model was established via combined transdermal [calcipotriol ointment mixed with ovalbumin (OVA)] and oral OVA challenges. The prevention group received daily oral gavage of a suspension of L. sulphureus (2×109 CFU/day) from the initiation of the experiment, whereas the treatment group was administered the same dosage of the bacterial suspension for 4 weeks following successful model establishment. Systemic allergic responses were assessed by monitoring body temperature and clinical symptoms post-challenge and serum IgE and mMCP-1 levels were quantified using ELISA. In addition, histopathological changes were evaluated using hematoxylin and eosin and toluidine blue staining, and Western blotting was performed to analyze the TLR4/NF-κB/MyD88 signaling pathway in lung tissues to assess its potential role in the response of L. sulphureus to OVA-induced FA. Results: Compared with the control mice, those in the FA group were characterized by hypothermia, elevated levels of IgE and mMCP-1, intestinal mucosal damage, mast cell infiltration, and pulmonary vascular dilation, along with the upregulated expression of TLR4, phosphorylated NF-κB, and MyD88. Intervention with L. sulphureus was found to contribute to a significant mitigation of the aforementioned adverse effects, reducing hypothermia, lowering IgE/mMCP-1 levels, attenuating tissue damage, and downregulating activation of the TLR4/NF-κB/MyD88 pathway. Conclusion: Laetiporus sulphureus can contribute to alleviating OVA-induced FA in mice by modulating the TLR4/NF-κB/MyD88 pathway, thereby indicating the potential utility of this bacterium as a therapeutic agent for the treatment of food allergies.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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