To optimize the extraction process for total flavonoids from pomelo flowers and investigate their antioxidant activity.
The extraction process of total flavonoids, the bioactive constituents from pomelo flower extracts, was optimized by single-factor experiments and orthogonal tests, while their antioxidant activities were evaluated.
The optimal extraction process conditions of total flavonoids from pomelo flowers were as follows:solid-liquid ratio 1:30 (g:mL), ethanol volume fraction 70% and ultrasonic time 50 min. Under these conditions, the extraction rate of total flavonoids was 12.44%. When the total flavonoid content was 49.86 mg/g, the scavenging rates of 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) disodium salt kation (ABTS+) and 1,1-diphenyl-2-picrylhydrazyl(DPPH) radicals reached 93.91% and 92.59%, respectively. The scavenging rate of hydroxyl radicals was as high as 99.51%, when the total flavonoid content was 24.93 mg/g.
Total flavonoids extracted from pomelo flowers exhibit excellent antioxidant capacity and possess superior application value in the food industry.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |