Full
Outline
To establish a method for the rapid detection of azithromycin residues in animal-derived foods by colloidal gold immunochromatographic assay (GICA).
By optimizing the pretreatment and the conditions of the test strip chromatography, the GICA was applied to the rapid detection of azithromycin in chicken, fish, pork, beef, lamb and the respective products. The limit of detection, sensitivity, false positive rate, false negative rate and cross reaction of the method were evaluated, and the accuracy was confirmed by liquid chromatography-tandem mass spectrometry(LC-MS/MS).
In this study of the rapid detection method, the limit of detection of azithromycin in animal-derived foods was 0.5 μg/kg, with the sensitivity 100%, false positive rate less than or equal to 2%, false negative rate 0%. There was no cross-reaction with gamithromycin, tulathromycin, tylvalosin, kitasamycin, erythromycin, spiramycin and tilmicosin. The coincidence rate between the test results and LC-MS/MS of actual samples was 100%.
The method is accurate, convenient, rapid and cheap, which is suitable for the high-throughput on-site rapid detection of azithromycin residues in animal-derived foods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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