Full
Outline
To establish a method for concurrent qualitative screening of 6 kinds of new phenylethylamine psychoactive substances in apple juice by liquid chromatography-quadrupole-time of flight mass spectrometry.
The samples were extracted with 10 mL acetonitrile, purified with C18 powder, and separated on a C18 column (100 mm×2.1 mm, 2.7 μm). The 10 mmol/L ammonium acetate aqueous solution (0.1% formic acid) and acetonitrile was as mobile phase, and the elution method was gradient elution. Mass spectrometry methods included electrospray ionization, positive ion scanning and information-dependent acquisition (IDA) mode detection. Screening was conducted by considering multiple factors, including the mass deviation, retention time,isotope distribution characteristics and similarity to the secondary spectrum library of the target signal.
The 6 kinds of targets showed good linearity (0.1–50.0 μg/L, r>0.9990) with matrix effects was all less than 20%, which could be classified as weak matrix effects. The limit of detection was 5.0 μg/L. The comprehensive scores of the qualitative screening were all above 90 points, meeting the requirements for qualitative confirmation.
The method is simple, rapid and sensitive, and can be used for the qualitative screening and detection of 6 kinds of new phenylethylamine psychoactive substances in apple juice.
7
1
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
