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Optimization of the culture medium and screening of freeze-drying protectants for Pediococcus acidilactici TC-4
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Zi-Yao ZHANG1, 2, Yu HAO1, 2, Ling CHEN2, 3, Ying LI2, 3, Xin-Qiang XIE2, Ting-Ting LIANG2, Chao-Xin MAN1, *, Qing-Ping WU1, 2, *
Journal of Food Safety & Quality | 2026, 17(10) : 231 - 243
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Journal of Food Safety & Quality | 2026, 17(10): 231-243
• Food Analysis and Detection •
Optimization of the culture medium and screening of freeze-drying protectants for Pediococcus acidilactici TC-4
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Zi-Yao ZHANG1, 2, Yu HAO1, 2, Ling CHEN2, 3, Ying LI2, 3, Xin-Qiang XIE2, Ting-Ting LIANG2, Chao-Xin MAN1, *, Qing-Ping WU1, 2, *
Affiliations
  • 1.College of Food Science, Northeast Agricultural University, Harbin 150030, China
  • 2.State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food, Key Laboratory of Big Data Technologies for Food Microbiological Safety, State Administration for Market Regulation, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
  • 3.Guangdong Microbial Analysis and Detection Center,Guangzhou 510070, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260319004
Outline
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Objective

To optimize the composition of the culture medium for Pediococcus acidilactici TC-4 and the formula of freeze-drying protectants.

Methods

The composition and mass concentration of the culture medium for Pediococcus acidilactici TC-4 and the formula of the freeze-drying protectant were screened by combining single-factor experiments, Plackett-Burman experiments and response surface methodology.

Results

The optimal composition of the culture medium was: Glucose 26 g/L, yeast extract 30 g/L, sodium acetate 8 g/L, diammonium hydrogen citrate 2 g/L, dipotassium phosphate 2 g/L, magnesium sulfate 0.4 g/L, manganese sulfate 0.075 g/L and Tween 80 1 g/L. The viable count increased from 3.50×109 CFU/mL to 1.09×1010 CFU/mL, which was 3.11 times that before optimization. The optimal formula of the freeze-drying protectant was: Sodium L-glutamate 8%, maltitol 14%,skim milk powder 18%, and the survival rate reached 90.77%.

Conclusion

The optimization of the culture medium for Pediococcus acidilactici TC-4 is completed, and a high-viable probiotic preparation is successfully prepared, lays a solid theoretical foundation for the industrialization of this strain.

Pediococcus acidilactici  /  hyperuricemia  /  culture medium  /  freeze-drying protectant
张仔瑶, 郝宇, 陈玲, 李滢, 谢新强, 梁婷婷, 满朝新, 吴清平. 乳酸片球菌TC-4的培养基优化及冻干保护剂筛选. 食品安全质量检测学报, 2026 , 17 (10) : 231 -243 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260319004
Zi-Yao ZHANG, Yu HAO, Ling CHEN, Ying LI, Xin-Qiang XIE, Ting-Ting LIANG, Chao-Xin MAN, Qing-Ping WU. Optimization of the culture medium and screening of freeze-drying protectants for Pediococcus acidilactici TC-4[J]. Journal of Food Safety & Quality, 2026 , 17 (10) : 231 -243 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260319004
Appendix
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Year 2026 volume 17 Issue 10
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260319004
  • Receive Date：2026-03-19
  • Online Date：2026-11-05
Article Data
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  • Received：2026-03-19
Funding
Affiliations
    1.College of Food Science, Northeast Agricultural University, Harbin 150030, China
    2.State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food, Key Laboratory of Big Data Technologies for Food Microbiological Safety, State Administration for Market Regulation, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
    3.Guangdong Microbial Analysis and Detection Center,Guangzhou 510070, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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