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Study on the effect of moderate enzymatic hydrolysis on the quality improvement of walnut milk
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Da-Xia YANG1, 2, Dong-Dong LIU3, 4, Jia-Xin YIN3, 5, Bing QI1, 2, Ze-Yu ZHAO3, Zhi-Heng YANG1, 6, Dong-Dong WANG1, 6, Jun-Xia XIA1, 2, Jian-Kang DENG3, 4, *
Journal of Food Safety & Quality | 2026, 17(4) : 12 - 20
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Journal of Food Safety & Quality | 2026, 17(4): 12-20
• Special Topic: Food Quality Analysis and Evaluation •
Study on the effect of moderate enzymatic hydrolysis on the quality improvement of walnut milk
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Da-Xia YANG1, 2, Dong-Dong LIU3, 4, Jia-Xin YIN3, 5, Bing QI1, 2, Ze-Yu ZHAO3, Zhi-Heng YANG1, 6, Dong-Dong WANG1, 6, Jun-Xia XIA1, 2, Jian-Kang DENG3, 4, *
Affiliations
  • 1.Hebei Yangyuan Zhihui Beverage Co., Ltd., Hengshui 053000, China
  • 2.Hebei Technology Innovation Center for Walnut Beverage, Hengshui 053000, China
  • 3.College of Life Sciences, Hengshui University, Hengshui 053000, China
  • 4.College of Life Sciences and Food Engineering, Huaiyin Institute of Technology, Huai’an 223000, China
  • 5.College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • 6.Hebei Provincial Key Laboratory of Walnut Nutrition Function and Processing Technology, Hengshui 053000, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20250916006
Outline
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Objective

To investigate the effect of moderate enzymatic hydrolysis process on the quality of walnut milk.

Methods

Walnut milk was treated with flavor protease and alkaline protease (the ratio was 2:1) under different enzymatic hydrolysis times: Blank control (no enzyme), 0 min (enzyme added and immediately inactivated), 30 min, 60 min and 120 min. The nutritional components, physical stability, aroma components and sensory quality of walnut milk were systematically analyzed.

Results

The nutritional components of walnut milk treated under different enzymatic hydrolysis times were different. With the increase of enzymatic hydrolysis time, the content of oligopeptides and free amino acids in the samples increased, reaching the highest levels at 120 min. The stability kinetic index of walnut milk after enzymatic hydrolysis for 30 min was the lowest and the stability was better.Enzymatic hydrolysis improved the aroma characteristics of walnut milk, increasing both the diversity and abundance of volatile compounds. Sensory evaluation showed that enzymatic hydrolysis enhanced the aroma intensity of walnut milk and the mouthfeel became smoother and more delicate, with the 30 min hydrolysate receiving the best overall acceptability.

Conclusion

Enzymatic hydrolysis for 30 min is the optimal time that provides the best overall balance for walnut milk. At this time, the nutritional composition, stability, flavor and sensory quality of walnut milk are improved.

walnut milk  /  enzymatic hydrolysis  /  quality
杨大霞, 刘东东, 尹佳欣, 齐兵, 赵泽宇, 杨志恒, 王东东, 夏君霞, 邓健康. 适度酶解对核桃乳的品质改良作用研究. 食品安全质量检测学报, 2026 , 17 (4) : 12 -20 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250916006
Da-Xia YANG, Dong-Dong LIU, Jia-Xin YIN, Bing QI, Ze-Yu ZHAO, Zhi-Heng YANG, Dong-Dong WANG, Jun-Xia XIA, Jian-Kang DENG. Study on the effect of moderate enzymatic hydrolysis on the quality improvement of walnut milk[J]. Journal of Food Safety & Quality, 2026 , 17 (4) : 12 -20 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250916006
Year 2026 volume 17 Issue 4
6
1
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doi: 10.19812/j.cnki.jfsq11-5956/ts.20250916006
  • Receive Date:2025-09-16
  • Online Date:2026-11-05
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History
  • Received:2025-09-16
Funding
Affiliations
    1.Hebei Yangyuan Zhihui Beverage Co., Ltd., Hengshui 053000, China
    2.Hebei Technology Innovation Center for Walnut Beverage, Hengshui 053000, China
    3.College of Life Sciences, Hengshui University, Hengshui 053000, China
    4.College of Life Sciences and Food Engineering, Huaiyin Institute of Technology, Huai’an 223000, China
    5.College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
    6.Hebei Provincial Key Laboratory of Walnut Nutrition Function and Processing Technology, Hengshui 053000, China
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表12种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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