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Outline
To analyze the characteristic components and antibacterial effects of Eucalyptus honey.
This study employed high performance liquid chromatography-tandem mass spectrometry for both qualitative and quantitative analysis of the characteristic components in Eucalyptus honey. Additionally, the antibacterial activity of Eucalyptus honey against Escherichia coli and Staphylococcus aureus were explored.
The 7 compounds were identified in Eucalyptus honey, including dehydrovomifoliol, 3-oxo-α-lonol, 3’-methoxyquercetin,3’,4’,5’,5,7-pentahydroxyflavone, 3’,5’,2,5,7-pentahydroxyflavone, luteolin and N1-p-coumaroylglucoside-N10-p-methoxycinnamoyl spermidine, among them, N1-p-coumaroylglucoside-N10-p-methoxycinnamoyl spermidine was reported for the first time in honey. The quantitative analysis results indicated that dehydrovomifoliol and 3-oxo-α-lonol were the main components. Through the antibacterial experiment and minimum inhibitory concentration determination,Eucalyptus honey exhibited antibacterial activity against Staphylococcus aureus at a concentration of 5.0 mg/mL and inhibitory effects against Escherichia coli at a concentration of 7.5 mg/mL.
This study conductes a systematic qualitative and quantitative analysis of the phytochemical compounds in Eucalyptus honey and demonstrates its favorable antimicrobial efficacy. Eucalyptus honey exhibites notable inhibitory activity against Staphylococcus aureus and Escherichia coli. The research provides a theoretical foundation for the authenticity assessment, quality control and functional product development of Eucalyptus honey.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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